Synthesis of Urea Sensors using Potentiometric Methods with Modification of Electrode Membranes Indicators of ISE from PVA-Enzymes Coating PVC-KT<sub>p</sub>ClPB

Hakim, Syamsul; Tarigan, Kerista; Situmorang, Manihar; Sembiring, Timbangen

doi:10.1088/1742-6596/1120/1/012024

Cited by 6 publications

(5 citation statements)

References 16 publications

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“…According to the specificities of target molecules, there are several types of potentiometric biosensors. Indeed, such electrochemical measurements have provided the evaluation of several vital biomolecules, such as organic and inorganic species, like sugars [ 35 ], urea [ 36 ], antibiotics [ 37 ], neurotransmitters [ 38 ], environment pollutants [ 39 ], carbon dioxide [ 26 ] and many ionic species, like salts and minerals [ 17 , 26 ]. Therefore, it is regularly used to determine the analytical concentration of biologically relevant electrolytes in physiological fluids, such as Na + , K + , Ca 2+ , and Cl − , which are responsible amongst other functions, for conduct electrical impulses in the body [ 17 , 40 ].…”

Section: Electrochemical Methods Employed On Disease Diagnosismentioning

confidence: 99%

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Biomolecules and Electrochemical Tools in Chronic Non-Communicable Disease Surveillance: A Systematic Review

et al. 2020

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Over recent three decades, the electrochemical techniques have become widely used in biological identification and detection, because it presents optimum features for efficient and sensitive molecular detection of organic compounds, being able to trace quantities with a minimum of reagents and sample manipulation. Given these special features, electrochemical techniques are regularly exploited in disease diagnosis and monitoring. Specifically, amperometric electrochemical analysis has proven to be quite suitable for the detection of physiological biomarkers in monitoring health conditions, as well as toward the control of reactive oxygen species released in the course of oxidative burst during inflammatory events. Besides, electrochemical detection techniques involve a simple and swift assessment that provides a low detection-limit for most of the molecules enclosed biological fluids and related to non-transmittable morbidities.

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Section: Electrochemical Methods Employed On Disease Diagnosismentioning

confidence: 99%

“…The most relevant parameters obtained by impedance spectroscopy [ 36 ]: The ohmic resistance of the analysed solution, Capacitance and charge distribution, of the interface electrode/solution, The electrochemical reaction rate-constant. …”

Section: Electrochemical Methods Employed On Disease Diagnosismentioning

confidence: 99%

Biomolecules and Electrochemical Tools in Chronic Non-Communicable Disease Surveillance: A Systematic Review

et al. 2020

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“…This electrode analysis has been characterized using SEM-EDX [2]. Synthesis of buffer solution KH 2 PO 4 +KCl [3]. The sensitivity, detection range and detection limit are problems in this study.…”

Section: Introductionmentioning

confidence: 99%

Characterization of manufacturing indicator electrodes coated PVA-enzyme coated 1X GA and 3X GA 2.9 % coated PVC-KTpClPB coated using potentiometry method

Mihardi

Rais

et al. 2022

J. Phys.: Conf. Ser.

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Research has been carried out on the manufacture of tungsten indicator electrodes with a diameter of 1 mm coated with PVA-Enzyme coated with PVC-KTpClPB using the biosensor potentiometer method. It has a sensitivity of 19,096 mV/decade, a detection range of 1.10-5 - 5.10-4 M, a detection limit of 1.10-5 M and a correlation coefficient of R2 = 0.9431. This detection range is very small, only a difference of one order to the negative power. On the basis of this small range, the PVA-Enzyme and PVA-Enzyme-GA solutions have been characterized by UV-vis showing a very clear difference in the width of the absorbance peak at wavelength. The indicator electrodes B1-4 and B3-4 have been analyzed using UV-vis, FTIR, SEM-eds and XRD. The method used is the biosensor poteniometric method, urease enzyme immobilization technique, which analytes urea in determining sensitivity, detection range and detection limit, as well as variable signal analysis of 60 signals/second. The results obtained from the indicator electrode B1-4 have a sensitivity of 46.67 mV/decade, a detection range of 10-4 - 10-2 M and a detection limit of 10-4 M, R2 = 99.62 %. The indicator electrode B3-4 has a sensitivity of 38.16 mV/decade, a detection range of 10-6 - 10-4 M and a detection limit of 10-6 M, R2 = 95.42 %. The best results on electrodes B1-4.

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“…Research has been carried out on indicator electrodes coated with PVA-enzyme coated with PVC-KTpClPB with a detection range of 10-5-10-4M (Hakim, 2019). This electrode analysis has been characterized using SEM-EDS (Hakim, 2018). According to (Rahman, 2008) the range is 5.10-7-10-2M.…”

Section: Introductionmentioning

confidence: 99%

Characterization of PVA-Enzyme Coated Indicator Electrodes GA coated again with PVC-KTpClPB-o-NPOE SEM-EDS, FTIR and XRD analysis

2022

jppipa, pendidikan ipa, fisika, biologi, kimia

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This study aims to characterize the tungsten-urea analyte indicator electrode. The method used is biosensor potentiometry with urease enzyme immobilization technique. This indicator electrode was coated with PVA-enzyme coated with glutaraldehyde (GA) 2.9% coated with PVC-KTpClPB- o-NPOE with o-NPOE variation of 61% and 66%. Characterization of coated indicator electrodes using SEM-EDS, FTIR and XRD analysis. A1-4 61% indicator electrode sample coated PVA-enzyme 1x coated with glutaraldehyde (GA) 2.9% 1x coated PVC-KTpClPB- o-NPOE 1x, with o-NPOE 61%. A3-4 61% indicator electrode sample coated PVA-enzyme 3x coated with glutaraldehyde (GA) 2.9% 1x coated PVC-KTpClPB- o-NPOE 1x, with o-NPOE 61%. Likewise, the reasoning of samples A1-4 66% and A3-4 66%. There are four indicator electrodes made with the notation A1-4 61%, A1-4 66%, A3-4 61% and A3-4 66%. The best results were obtained at the indicator electrode sample A1-4 61%, contributing to the urea sensor of the potentiometer cell

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Synthesis of Urea Sensors using Potentiometric Methods with Modification of Electrode Membranes Indicators of ISE from PVA-Enzymes Coating PVC-KT_pClPB

Cited by 6 publications

References 16 publications

Biomolecules and Electrochemical Tools in Chronic Non-Communicable Disease Surveillance: A Systematic Review

Biomolecules and Electrochemical Tools in Chronic Non-Communicable Disease Surveillance: A Systematic Review

Characterization of manufacturing indicator electrodes coated PVA-enzyme coated 1X GA and 3X GA 2.9 % coated PVC-KTpClPB coated using potentiometry method

Characterization of PVA-Enzyme Coated Indicator Electrodes GA coated again with PVC-KTpClPB-o-NPOE SEM-EDS, FTIR and XRD analysis

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Synthesis of Urea Sensors using Potentiometric Methods with Modification of Electrode Membranes Indicators of ISE from PVA-Enzymes Coating PVC-KTpClPB

Cited by 6 publications

References 16 publications

Biomolecules and Electrochemical Tools in Chronic Non-Communicable Disease Surveillance: A Systematic Review

Biomolecules and Electrochemical Tools in Chronic Non-Communicable Disease Surveillance: A Systematic Review

Characterization of manufacturing indicator electrodes coated PVA-enzyme coated 1X GA and 3X GA 2.9 % coated PVC-KTpClPB coated using potentiometry method

Characterization of PVA-Enzyme Coated Indicator Electrodes GA coated again with PVC-KTpClPB-o-NPOE SEM-EDS, FTIR and XRD analysis

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Synthesis of Urea Sensors using Potentiometric Methods with Modification of Electrode Membranes Indicators of ISE from PVA-Enzymes Coating PVC-KT_pClPB