Synthesis of l-aspartic acid-based bimetallic hybrid nanoflowers to immobilize snailase for the production of rare ginsenoside compound K

Abstract

The conversion of the common ginsenoside Rb1 to the rare ginsenoside compound K (CK) using snailase (Sna) is an efficient method for industrial production. In order to improve the...

A two-enzyme system in an amorphous metal–organic framework for the synthesis of d-phenyllactic acid

“…The second stage is the major quality loss stage. In the range of 200-800 1C, the loss of mass is mainly caused by the decomposition of the crystal structure 17,32,33 (ZIF-90 and LDH/GDH-ZIF-90 mass losses of 59.3% and 62.6%, respectively). However, the thermogravimetric analysis curves of LDH/GDH-ZIF-90 and ZIF-90 showed mainly two significant mass loss behaviors, which were differed from the above results (Fig.…”

Catechol-tetraethylenepentamine co-deposition modified cellulose filter paper for α-glucosidase immobilization and inhibitor screening from traditional Chinese medicine

“…This strategy not only stabilizes the enzyme structure fundamentally, but also enables continuous operation and rapid termination of enzymatic reactions. With the rapid development of materials science and technology, enzyme immobilization could be realized on various carrier materials, such as meta-organic frameworks (MOFs), 11 covalent organic frameworks (COFs), 12 carbon nanotubes (CNTs), 13 nanoflowers, 14 and other inorganic particles. However, for nanoscopic carriers, tedious operations such as centrifugation or filtration are required to separate the immobilized enzyme from the enzymatic reaction system due to their small size and low density, which are adverse to the termination of enzymatic reactions and the recycling use of the immobilized enzyme.…”

Residue Effect-Guided Design: Engineering of S. Solfataricus β-Glycosidase to Enhance Its Thermostability and Bioproduction of Ginsenoside Compound K

“…For the efficient production of CK, a one-pot multienzyme-catalyzed strategy based on BG23 (β-glucosidase from Aspergillus tubingensis ) and BGA35 (β-galactosidase from Aspergillus oryzae) was established . The conversion of ginsenoside Rb1 to CK by snailase (Sna) has also been demonstrated as an effective industrial production method based on a new immobilization approach to improve the catalyst morphology and enzyme activity …”

“…9 The conversion of ginsenoside Rb1 to CK by snailase (Sna) has also been demonstrated as an effective industrial production method based on a new immobilization approach to improve the catalyst morphology and enzyme activity. 10 SS-BGL is implicated as a highly suitable enzyme for the CK production process. In accordance with previous literature, 11 as many as 19 enzymes are known that prepare CK with ginsenoside Rb1 as the substrate, among which six βglycosidases have been extracted and purified.…”