Catechol-tetraethylenepentamine co-deposition modified cellulose filter paper for α-glucosidase immobilization and inhibitor screening from traditional Chinese medicine
Guang-Zhen Wan,
Chun-Lin Zhang,
Juan Chen
Abstract:Cellulose filter paper (CFP) is expected to be an ideal carrier for enzyme immobilization due to its sustainability and biocompatibility.
“…Additionally, compared to other immobilized α-glucosidase materials, the ZIF-8@PDA@PVDF system demonstrates significantly superior reusability. For instance, after being reused 10 times, enzyme activity of only 72.4% and 68.4% was retained for magnetic porous organic polymer 30 and cellulose filter paper 31 immobilized α-glucosidase, respectively.…”
In this study, a novel screening approach is presented, merging capillary electrophoresis analysis with an enzymatic assay employing immobilized α-glucosidase.
“…Additionally, compared to other immobilized α-glucosidase materials, the ZIF-8@PDA@PVDF system demonstrates significantly superior reusability. For instance, after being reused 10 times, enzyme activity of only 72.4% and 68.4% was retained for magnetic porous organic polymer 30 and cellulose filter paper 31 immobilized α-glucosidase, respectively.…”
In this study, a novel screening approach is presented, merging capillary electrophoresis analysis with an enzymatic assay employing immobilized α-glucosidase.
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