Synthesis of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) with high 4HB composition and PHA content using 1,4-butanediol and 1,6-hexanediol for medical application

Norhafini, Hambali; Thinagaran, L.; Shantini, K.; Huong, Kai‐Hee; Syafiq, Ishak Muhammad; Bhubalan, Kesaven; Amirul, Al-Ashraf Abdullah

doi:10.1007/s10965-017-1345-x

Cited by 24 publications

(18 citation statements)

References 28 publications

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“…The microorganisms Cupriavidus malaysiensis USMAA1020 transformant were grown in nutrient-rich (NR) broth (10 g of peptone, 10 g of lab-lemco powder and 2 g of yeast extract in 1 L distilled water) for 12 hours and then transferred 0.1 g/L of the bacteria culture into nitrogen-free mineral salts medium (MSM) [1]. The mixture of both 1,4-butanediol and 1,6-hexanediol were used as the 4HB carbon precursors for the biosynthesis [2]. After 84 hours cultivation, the cultures were harvested by centrifugation at 10000 rpm for 15 minutes and then freeze-dried.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Data on the effect of electrospinning parameters on the morphology of the nanofibrous poly(3-hydroxybutyrate-co-4-hydroxybutyrate) scaffolds

2020

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Electrospinning is a promising approach to fabricate desirable electropsun nanofibrous scaffold that could be applied in the medical fields. In this study, bacterial copolymer poly(3-hydroxybutyrate-co-68 mol% 4-hydroxybutyrate) [P(3HB-co-68mol% 4HB)] copolymer produced was fabricated into electrospun nanofibers using various combination of electrospinning parameters including the polymer solution, applied voltage and injection speed. The morphology of the fabricated scaffolds were observed using scanning electron microscope (SEM). The SEM images were analysed for the fibre diameter distribution of the scaffolds using Image Analyser. The results revealed that the 8 wt% of polymer solution, 25 kV/cm of the applied voltage and 1.5 mL/h of the injection speed was the most suitable combination. This electrospinning parameters combination fabricated nanofibrous P(3HB-co-4HB) scaffold with smooth, beadles and uniform nanofibers with small fibre diameter distribution.

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Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Data on the effect of electrospinning parameters on the morphology of the nanofibrous poly(3-hydroxybutyrate-co-4-hydroxybutyrate) scaffolds

2020

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“…A preculture of 5% (v/v) of the working volume was transferred into 20 L fermenter (Biostat ® C plus, Sartorius Stedim, German) containing mineral salts medium (MSM) with carbon precursors (1,4-butanediol and 1,6-hexanediol in the 1:5 ratio). The fermentation was carried out at 30 • C with an agitation speed of 200 rpm, the aeration rate of 1 vvm and controlled pH of 7 for 108 h. Sampling was done at intervals of every 12 h. The composition of PHA produced was determined by gas chromatography (GC) using Shimadzu Gas Chromatography GC-2014 according to methods previously described [24]. Endotoxin removal was carried out on extracted P(3HB-co-95 mol% 4HB) copolymer as previously described.…”

Section: Biosynthesis Of P(3hb-co-95 Mol% 4hb) Copolymermentioning

confidence: 99%

Surface Characterization and Physiochemical Evaluation of P(3HB-co-4HB)-Collagen Peptide Scaffolds with Silver Sulfadiazine as Antimicrobial Agent for Potential Infection-Resistance Biomaterial

et al. 2021

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Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] is a bacterial derived biopolymer widely known for its unique physical and mechanical properties to be used in biomedical application. In this study, antimicrobial agent silver sulfadiazine (SSD) coat/collagen peptide coat-P(3HB-co-4HB) (SCCC) and SSD blend/collagen peptide coat-P(3HB-co-4HB) scaffolds (SBCC) were fabricated using a green salt leaching technique combined with freeze-drying. This was then followed by the incorporation of collagen peptides at various concentrations (2.5–12.5 wt.%) to P(3HB-co-4HB) using collagen-coating. As a result, two types of P(3HB-co-4HB) scaffolds were fabricated, including SCCC and SBCC scaffolds. The increasing concentrations of collagen peptides from 2.5 wt.% to 12.5 wt.% exhibited a decline in their porosity. The wettability and hydrophilicity increased as the concentration of collagen peptides in the scaffolds increased. In terms of the cytotoxic results, MTS assay demonstrated the L929 fibroblast scaffolds adhered well to the fabricated scaffolds. The 10 wt.% collagen peptides coated SCCC and SBCC scaffolds displayed highest cell proliferation rate. The antimicrobial analysis of the fabricated scaffolds exhibited 100% inhibition towards various pathogenic microorganisms. However, the SCCC scaffold exhibited 100% inhibition between 12 and 24 h, but the SBCC scaffolds with SSD impregnated in the scaffold had controlled release of the antimicrobial agent. Thus, this study will elucidate the surface interface-cell interactions of the SSD-P(3HB-co-4HB)-collagen peptide scaffolds and controlled release of SSD, antimicrobial agent.

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“…In the biosynthesis pathway, 1,4-butanediol is first oxidised to 4-hydroxybutyric acid, subsequently into 4-hydroxybutyryl-CoA [ 54 ]. In concept, ω-alkanediols are oxidised into the corresponding ω-hydroxy fatty acid, then converted into coenzyme A thioester [ 65 ].…”

Section: Synthesis Of Poly(3-hydroxybutyrate -Co- mentioning

confidence: 99%

“…4-Hydroxybutyryl-CoA is directly polymerised by PHA synthase since it is not a chiral intermediate compared to 3-hydroxybutyryl-CoA [ 65 ]. A part of 4-hydroxybutyryl-CoA is converted into 4HB, while another part undergoes a complex pathway converting it into 3-hydroxybutyryl-CoA, subsequently forming 3HB monomer, which incorporates with 4HB to produce P(3HB -co- 4HB) [ 54 ].…”

Section: Synthesis Of Poly(3-hydroxybutyrate -Co- mentioning

confidence: 99%

Surface-Modified Highly Biocompatible Bacterial-poly(3-hydroxybutyrate-co-4-hydroxybutyrate): A Review on the Promising Next-Generation Biomaterial

et al. 2020

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Polyhydroxyalkanoates (PHAs) are bacteria derived bio-based polymers that are synthesised under limited conditions of nutritional elements with excess carbon sources. Among the members of PHAs, poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [(P(3HB-co-4HB)] emerges as an attractive biomaterial to be applied in medical applications owing to its desirable mechanical and physical properties, non-genotoxicity and biocompatibility eliciting appropriate host tissue responses. The tailorable physical and chemical properties and easy surface functionalisation of P(3HB-co-4HB) increase its practicality to be developed as functional medical substitutes. However, its applicability is sometimes limited due to its hydrophobic nature due to fewer bio-recognition sites. In this review, we demonstrate how surface modifications of PHAs, mainly P(3HB-co-4HB), will overcome these limitations and facilitate their use in diverse medical applications. The integration of nanotechnology has drastically enhanced the functionality of P(3HB-co-4HB) biomaterials for application in complex biological environments of the human body. The design of versatile P(3HB-co-4HB) materials with surface modifications promise a non-cytotoxic and biocompatible material without inducing severe inflammatory responses for enhanced effective alternatives in healthcare biotechnology. The enticing work carried out with P(3HB-co-4HB) promises to be one of the next-generation materials in biomedicines which will facilitate translation into the clinic in the future.

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Synthesis of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) with high 4HB composition and PHA content using 1,4-butanediol and 1,6-hexanediol for medical application

Cited by 24 publications

References 28 publications

Data on the effect of electrospinning parameters on the morphology of the nanofibrous poly(3-hydroxybutyrate-co-4-hydroxybutyrate) scaffolds

Data on the effect of electrospinning parameters on the morphology of the nanofibrous poly(3-hydroxybutyrate-co-4-hydroxybutyrate) scaffolds

Surface Characterization and Physiochemical Evaluation of P(3HB-co-4HB)-Collagen Peptide Scaffolds with Silver Sulfadiazine as Antimicrobial Agent for Potential Infection-Resistance Biomaterial

Surface-Modified Highly Biocompatible Bacterial-poly(3-hydroxybutyrate-co-4-hydroxybutyrate): A Review on the Promising Next-Generation Biomaterial

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