Data on the effect of electrospinning parameters on the morphology of the nanofibrous poly(3-hydroxybutyrate-co-4-hydroxybutyrate) scaffolds

Chai, Carmil; Amirul, Al-Ashraf Abdullah; Vigneswari, Sevakumaran

doi:10.1016/j.dib.2019.104777

Cited by 5 publications

(7 citation statements)

References 5 publications

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“…The smooth, uniform and beadless P(3HB-co-4HB) nanofibers were fabricated after a careful optimization of the polymer solution concentration and processing parameters (applied voltage and flow rate). The final conditions were polymer concentration 8 wt%, applied voltage 25 kV and flow rate 1.5 mL/h, in agreement with those reported by Chai et al (2020). However, as already anticipated, these scaffolds lack biological cell recognition sites which restricts the cell-material interaction limiting their applications.…”

Section: Fabrication Of Nano-p(3hb-co-4hb) and Immobilization Of Biomimetic Rgd Peptidessupporting

confidence: 86%

Elucidating the Surface Functionality of Biomimetic RGD Peptides Immobilized on Nano-P(3HB-co-4HB) for H9c2 Myoblast Cell Proliferation

Vigneswari

Chai

Kamarudin

et al. 2020

Front. Bioeng. Biotechnol.

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Biomaterial scaffolds play crucial role to promote cell proliferation and foster the regeneration of new tissues. The progress in material science has paved the way for the generation of ingenious biomaterials. However, these biomaterials require further optimization to be effectively used in existing clinical treatments. It is crucial to develop biomaterials which mimics structure that can be actively involved in delivering signals to cells for the formation of the regenerated tissue. In this research we nanoengineered a functional scaffold to support the proliferation of myoblast cells. Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] copolymer is chosen as scaffold material owing to its desirable mechanical and physical properties combined with good biocompatibility, thus eliciting appropriate host tissue responses. In this study P(3HB-co-4HB) copolymer was biosynthesized using Cupriavidus malaysiensis USMAA1020 transformant harboring additional PHA synthase gene, and the viability of a novel P(3HB-co-4HB) electrospun nanofiber scaffold, surface functionalized with RGD peptides, was explored. In order to immobilize RGD peptides molecules onto the P(3HB-co-4HB) nanofibers surface, an aminolysis reaction was performed. The nanoengineered scaffolds were characterized using SEM, organic elemental analysis (CHN analysis), FTIR, surface wettability and their in vitro degradation behavior was evaluated. The cell culture study using H9c2 myoblast cells was conducted to assess the in vitro cellular response of the engineered scaffold. Our results demonstrated that nano-P(3HB-co-4HB)-RGD scaffold possessed an average fiber diameter distribution between 200 and 300 nm, closely biomimicking, from a morphological point of view, the structural ECM components, thus acting as potential ECM analogs. This study indicates that the surface conjugation of biomimetic RGD peptide to the nano-P(3HB-co-4HB)

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Section: Fabrication Of Nano-p(3hb-co-4hb) and Immobilization Of Biomimetic Rgd Peptidessupporting

confidence: 86%

Elucidating the Surface Functionality of Biomimetic RGD Peptides Immobilized on Nano-P(3HB-co-4HB) for H9c2 Myoblast Cell Proliferation

Vigneswari

Chai

Kamarudin

et al. 2020

Front. Bioeng. Biotechnol.

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“…Electrospinning at ambient conditions might have led to the observed differences as it was recently shown that variations in room temperature and/or differences in relative humidity can influence the surface structure of electrospun fibers . So far, P4HB has only been electrospun onto supporting membranes or in blends with other polymers. ,,, We thus believe that we were among the first to successfully electrospin P4HB into free-standing membranes. To achieve this, influencing parameters such as the polymer concentration, solvent systems and applied voltage, flow rate, and needle-to-collector distance were carefully adapted.…”

Section: Resultsmentioning

confidence: 91%

“…PHAs are biosynthesized by different bacterial species as natural energy storage of carbon compounds . Highly elastic properties (elongation at break of 1000%) combined with a tensile strength of 50 MPa make P4HB a promising candidate for various applications in the biomedical field such as sutures, hernia repair meshes, or scaffolds for plastic and reconstructive surgery. − P4HB degrades into 4HB, which is a naturally occurring metabolite that enters the Krebs cycle and is eventually converted to water and carbon dioxide. , Despite its many advantages and reported use in clinical scenarios, limited knowledge is available on electrospun P4HB, which was mainly used as coating on, e.g., decellularized heart valves, microstents, or poly(glycolic acid) meshes, ,− or electrospun as a copolymer with P3HB . Besides these reports, a patent from 2014 claims the production and use of ultrafine electrospun P4HB membranes for multiple biomedical applications; its implementation, however, remains open to date, and electrospun P4HB membranes have not been investigated as potential wound dressings.…”

Section: Introductionmentioning

confidence: 99%

Gallium Complex-Functionalized P4HB Fibers: A Trojan Horse to Fight Bacterial Infection

Müller

Fessele

Zuber

et al. 2020

ACS Appl. Bio Mater.

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Broadband administration and overuse of conventional antibiotics contribute to antibiotic resistance, demanding for alternative strategies. Metal ions, among them gallium (Ga), gained increasing interest as antibacterial agents. Ga can be complexed in a protoporphyrin ring (GaPPIX) and thereby shares similarities with the heme molecule and functions as a Trojan horse that is internalized by bacteria. Different to iron in heme, Ga interferes with the metabolism, induces bacterial death, and can therefore be used as an antibacterial treatment. Here, electrospun membranes of poly(4-hydroxybutyrate) (P4HB) functionalized with GaPPIX were developed. P4HB was biosynthesized and spun into non-wovens, displaying fiber diameters of ∼700 nm. In vitro, GaPPIX-loaded membranes induced a median log 2 reduction of viable Staphylococcus aureus. In culture with human dermal fibroblasts, no cytotoxic effects were observed. These antibacterial membranes have the potential to contribute to alternative antibacterial strategies for the treatment of skin wounds.

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“…Prior to that the scaffolds were sterilized under UV (Spectrolinker™, XL-1000UV Cross linke, New York, NY, USA) at 1200 µJ/cm 2 for 30 min [ 12 ]. The cells viability and proliferation were assayed with MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium/PMS (phenazinmethosulfate) [ 16 , 17 ].…”

Section: Methodsmentioning

confidence: 99%

Elucidation of Antimicrobial Silver Sulfadiazine (SSD) Blend/Poly(3-Hydroxybutyrate-co-4-Hydroxybutyrate) Immobilised with Collagen Peptide as Potential Biomaterial

et al. 2020

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The quest for a suitable biomaterial for medical application and tissue regeneration has resulted in the extensive research of surface functionalization of material. Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] is a bacterial polymer well-known for its high levels of biocompatibility, non-genotoxicity, and minimal tissue response. We have designed a porous antimicrobial silver SSD blend/poly(3HB-co-4HB)-collagen peptide scaffold using a combination of simple techniques to develop a scaffold with an inter-connected microporous pore in this study. The collagen peptide was immobilised via -NH2 group via aminolysis. In order to improve the antimicrobial performance of the scaffold, silver sulfadiazine (SSD) was impregnated in the scaffolds. To confirm the immobilised collagen peptide and SSD, the scaffold was characterized using FTIR. Herein, based on the cell proliferation assay of the L929 fibroblast cells, enhanced bioactivity of the scaffold with improved wettability facilitated increased cell proliferation. The antimicrobial activity of the SSD blend/P(3HB-co-4HB)-collagen peptide in reference to the pathogenic Gram-negative, Gram-positive bacteria and yeast Candida albicans exhibited SSD blend/poly(3HB-co-4HB)-12.5 wt% collagen peptide as significant construct of biocompatible antibacterial biomaterials. Thus, SSD blend/P(3HB-co-4HB)-collagen peptide scaffold from this finding has high potential to be further developed as biomaterial.

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Data on the effect of electrospinning parameters on the morphology of the nanofibrous poly(3-hydroxybutyrate-co-4-hydroxybutyrate) scaffolds

Cited by 5 publications

References 5 publications

Elucidating the Surface Functionality of Biomimetic RGD Peptides Immobilized on Nano-P(3HB-co-4HB) for H9c2 Myoblast Cell Proliferation

Elucidating the Surface Functionality of Biomimetic RGD Peptides Immobilized on Nano-P(3HB-co-4HB) for H9c2 Myoblast Cell Proliferation

Gallium Complex-Functionalized P4HB Fibers: A Trojan Horse to Fight Bacterial Infection

Elucidation of Antimicrobial Silver Sulfadiazine (SSD) Blend/Poly(3-Hydroxybutyrate-co-4-Hydroxybutyrate) Immobilised with Collagen Peptide as Potential Biomaterial

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