We report a simple and effective synthetic method to prepare monodisperse indium metal nanoparticles less than 10 nm in size via a simple lithium borohydride reduction method conducted in amine based solvents. The method has advantages over literature methods; in particular, use of highly reactive and costly organoindium precursors and small gold clusters is required for the heterogeneous growth of sub-10-nm indium nanoparticles. Highly monodisperse spherical nanoparticles produced in one pot in our system could be selectively passivated with commonly available surfactants, including oleylamine, trioctylphosphine and trioctylphosphine oxide. The amine solvents could be readily moved by evaporation under reduced pressure and the particles easily redispersed in common organic solvent in colloidal form. Additionally, highly faceted polyhedra of indium metal could also be produced under specific conditions.
Biomaterial scaffolds play crucial role to promote cell proliferation and foster the regeneration of new tissues. The progress in material science has paved the way for the generation of ingenious biomaterials. However, these biomaterials require further optimization to be effectively used in existing clinical treatments. It is crucial to develop biomaterials which mimics structure that can be actively involved in delivering signals to cells for the formation of the regenerated tissue. In this research we nanoengineered a functional scaffold to support the proliferation of myoblast cells. Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] copolymer is chosen as scaffold material owing to its desirable mechanical and physical properties combined with good biocompatibility, thus eliciting appropriate host tissue responses. In this study P(3HB-co-4HB) copolymer was biosynthesized using Cupriavidus malaysiensis USMAA1020 transformant harboring additional PHA synthase gene, and the viability of a novel P(3HB-co-4HB) electrospun nanofiber scaffold, surface functionalized with RGD peptides, was explored. In order to immobilize RGD peptides molecules onto the P(3HB-co-4HB) nanofibers surface, an aminolysis reaction was performed. The nanoengineered scaffolds were characterized using SEM, organic elemental analysis (CHN analysis), FTIR, surface wettability and their in vitro degradation behavior was evaluated. The cell culture study using H9c2 myoblast cells was conducted to assess the in vitro cellular response of the engineered scaffold. Our results demonstrated that nano-P(3HB-co-4HB)-RGD scaffold possessed an average fiber diameter distribution between 200 and 300 nm, closely biomimicking, from a morphological point of view, the structural ECM components, thus acting as potential ECM analogs. This study indicates that the surface conjugation of biomimetic RGD peptide to the nano-P(3HB-co-4HB)
One-pot synthetic method was adopted to prepare three isomers 4-(
ortho-
fluorophenyl)thiosemi- carbazide), 4-(
meta-
fluorophenyl)thiosemicarbazide and 4-(
para-
fluorophenyl)thiosemicarbazide. The products were obtained in ethanolic solution from a reaction between
ortho, meta
and
para
derivatives of fluorophenyl isothiocyanate and hydrazine hydrate. This work presents the theoretical Molecular Electrostatic Potential (MEP) and Highest Occupied Molecular Orbital-Lowest Unoccupied Molecular Orbital (HOMO-LUMO) computational data through Gaussview 5.0.9 and Gaussian09 software. Experimental Cole-cole plot for conductivity determination was also illustrated. The present data is important to manipulate the properties of compounds according to the position of a fluorine atom.
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