Synthesis of platelet-activating factor and its receptor expression in Kupffer cells in rat carbon tetrachloride-induced cirrhosis

Abstract

AIM:To determine the platelet-activating factor (PAF) synthesis and its receptor expression in Kupffer cells in rat carbon tetrachloride-induced cirrhosis. METHODS:Kupffer cells, isolated from the livers of control and CCl4-induced cirrhotic rats, were placed in serum-free medium overnight. PAF saturation binding, ET-1 saturation and competition binding were assayed. ET-1 induced PAF synthesis, mRNA expression of PAF, preproendothelin-1, endothelin A (ETA) and endothelin B (ETB) receptors were also determined. RESULTS:

The protective effect of Zizyphus jujube fruit on carbon tetrachloride-induced hepatic injury in mice by anti-oxidative activities

“…Other products include conjugated dienes, lipid hydroperoxides, malonaldehydelike substances, and other short-chain hydrocarbons. In response to hepatocellular injury initiated by the biotransformation of CCl 4 to reactive radicals, "activated" Kupffer cells in liver respond by releasing increased amounts of active oxygen species and other bioactive agents (Lu et al, 2008).…”

Silymarin/Silybin and Chronic Liver Disease: A Marriage of Many Years

“…A crucial role in the progression of liver damage to the development of fibrosis and cirrhosis is carried out by platelet-activating factor (PAF), whose production, strictly related to the coexistence of tissue inflammation, allows HSC to produce a large quantity of collagen [ 121 , 122 , 123 , 124 ]. The capacity of remodelling ascribed to PAF, would be opposed by the process of acetylation supported by lysophosphatidylcholine acyltransferase enzymes (LPCAT), whose expression is clearly lower in cirrhotic patients compared to controls.…”

The hepatoprotective effect of coumarin and coumarin derivates on carbon tetrachloride-induced hepatic injury by antioxidative activities in rats

“…This sequence of events leads to liver injury. In response to this hepatocellular injury, "activated" hepatic Kupfer cells release increased quantities of active oxygen species and other bioactive agents [10,30]. Because free radicals and reactive oxygen species play a central role in liver disease pathology and progression, dietary antioxidants have been proposed as therapeutic agents to counteract liver damage [4,19,29].…”