The hepatoprotective effect of coumarin and coumarin derivates on carbon tetrachloride-induced hepatic injury by antioxidative activities in rats

Abstract

Coumarins are a vast group of natural compounds and some of them possess antioxidant activities. The comparison of the antioxidant activity of some coumarins with various chemical molecular structure has not been investigated in previous studies. Therefore, this study was aimed to investigate the hepatoprotective effect against carbon tetrachloride (CCl(4)) -induced hepatic injury by coumarin (1,2-benzopyrone) and coumarin derivatives, esculetin (6,7-dihydroxycoumarin), scoparone (6,7-dimethoxycoumarin), and 4-methylumbelliferone (7-hyroxy-4-methyl) in male Sprague-Dawley rats. Product of lipid peroxidation, malondialdehyde (MDA), activities of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT) were evaluated for oxidative stress in hepatic injury. Gamma glutamyl transpeptidase (GGT), lactate dehydrogenase (LDH) were detected in plasma as a biomarker of hepatic injury. Significantly elevated levels of MDA and lowered levels of SOD and CAT activities were observed in liver of rats exposed to CCl(4), when compared to control values. Similarly, administration of CCl(4) increased LDH and GGT levels in serum. Pre-treatment of rats with esculetin (35 mg kg(-1), orally) and scoparone (35 mg kg(-1), orally) significantly prevented CCl(4)-induced decrease in MDA levels and increase in SOD and CAT, whereas 4-methylumbelliferone (35 mg kg(-1)) and coumarin (30 mg kg(-1)) had no effect against CCl(4)-induced rise in serum enzymes. Esculetin and scoparone also showed protective properties as was evidenced in reduced LDH and GGT levels in serum. The results of this study indicate that the chemical structures of coumarins play an important role in the prevention of oxidative stress.

Antifibrotic effects of Artemisia capillaris and Artemisia iwayomogi in a carbon tetrachloride-induced chronic hepatic fibrosis animal model

“…1), scoparone is a main component in Artemisia capillaris and Artemisia iwayomogi, while scopoletin is strongly present in Artemisia iwayomogi (Seo et al, 2010). Both scoparone and scopoletin are known to have antioxidant activity (Thuong et al, 2010;Atmaca et al, 2011), and hepatoprotective effects (Kang et al, 1998;Murat Bilgin et al, 2011). Based on these publications, the two traditional hepatotherapeutic herbs seem to have hepatotherapeutic properties.…”

Simultaneous quantification and validation of new peroxynitrite scavengers fromArtemisia iwayomogi

“…The fact that scopoletin contained A. iwayomogi is active in osteoporosis (Lee et al, 2013) and hepatic diseases (Atmaca et al, 2011) was reported. However, it was revealed in the present study that the five compounds, chlorogenic acid, 3,4-dicaffeoylquinic acid, rutin, patuletin 3-O-glucoside, and 2 00 -O-caffeoylrutin, are also potent peroxynitrite scavengers, in addition to scopolin and scopoletin, which are already known to be peroxynitrite scavengers from A. iwayomogi.…”

Hepatoprotective activity of <i>Chenopodium murale</i> in carbon tetrachloride-induced hepatic damage in rabbits

“…(Ahmad and Eram, 2011). Coumarins in C. murale have potent anti-oxidant effect and hence strong hepatoprotective activity (Amaca et al, 2011). …”