Simultaneous quantification and validation of new peroxynitrite scavengers fromArtemisia iwayomogi

Abstract

Context: Artemisia iwayomogi Kitamura (Compositae) has been very widely used for the treatment of acute or chronic hepatitis, jaundice, and gastritis. In the course of our continuing efforts to identify and quantify peroxynitrite scavengers from Compositae plants, A. iwayomogi was used in this study. Objective: The present study was aimed to identify and quantify the peroxynitrite scavengers of A. iwayomogi. Materials and methods: Silica gel and ODS were used for column chromatography. The isolated compounds were quantified using an HPLC equipped with a Capcell Pak C18 column (5 mm, 250 mm  4.6 mm i.d.), and the method was validated for the quality control. Peroxynitrite (ONOO À )-scavenging activities of the compounds and extracts were evaluated on the measurement of highly fluorescent rhodamine 123 converted from non-fluorescent dihydrorhodamine (DHR)-123 under the presence of peroxynitrite. Results: Based on the spectroscopic evidences, a new compound, 2 00 -O-caffeoylrutin (2 00 -O-trans-caffeic acid ester of quercetin 3-O-a-L-rhamnopyranosyl(1 ! 6)-b-D-glucopyranoside) was isolated and determined together with patuletin 3-O-glucoside, scopolin, scopoletin, rutin, 3,4-dicaffeoylquinic acid, and chlorogenic acid. All of them were potent peroxynitrite scavengers (IC 50 1.88 mg/mL). Discussion and conclusion: The peroxynitrite scavengers were mainly distributed in the EtOAc fraction rather than the ether and BuOH fractions. The 70% MeOH extract exhibited a high peroxynitrite-scavenging activity. Through the validation, the present HPLC method was verified to be sufficiently sensitive, accurate, precise, and stable. Therefore, this method can be used for the quality control of A. iwayomogi.

Artemisiae Iwayomogii Herba Inhibits Growth, Motility, and the PI3K/AKT/mTOR Signaling Pathway in Hepatocellular Carcinoma Cells

“…Although AIE did not show higher inhibition of cell growth than that of sorafenib in HCC, we observed that AIE had capacity to inhibit cell growth of HCC with less cytotoxicity in normal cells. Scopoletin and isochlorogenic acid B are well-known compounds of AI as previously reported [14,15]. For this reason, these compounds were used to standardize AIE.…”

Chlorogenic acid inhibits hypoxia-induced angiogenesis via down-regulation of the HIF-1α/AKT pathway

“…coffee, potatoes and apples, and natural products containing CGA have widely been used for the treatment of inflammation [9][10][11] . CGA exerts diverse biological activities, including anti-inflammatory, anti-oxidative and anti-neurotoxic activities [12][13][14][15].…”

TOCSY, hydrogen decoupling and computational calculations to an unequivocal structural elucidation of a new sesquiterpene derivative and identification of other constituents from Praxelis sanctopaulensis

“…Compounds 2 and 3 were identified as steroids β ‐sitosterol 43 and stigmasterol, 43 respectively. Purification of ethyl acetate fraction on Sephadex LH‐20 CC afforded the flavonoids rutin ( 4 ), 44 2″‐ O ‐caffeoylrutin ( 5 ), 45 and hyperin ( 7 ) 46 . Compound 6 was identified as arbutin, a hydroquinone derivative 47,48 .…”