Synthesis of hapten and conjugates of coumestrol and development of immunoassay

Lapčı́k, Oldřich; Štursa, Jan; Kleinová, T.; Vítková, Michaela; Dvořáková, Hana; Klejdus, Bořivoj; Moravcová, Jitka

doi:10.1016/j.steroids.2003.08.014

Cited by 6 publications

(1 citation statement)

References 25 publications

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“…However, for large population studies HPLC does not show sufficient sensitivity, whereas GC/LC-MS is too laborious for screening of large numbers of samples. For this reason, immunoassays seem highly suitable and with regard to immunoassays for phytochemicals or phytoestrogens, the group of Adlercreutz and Lapčik has developed initially radioimmunoassays for daidzein, genistein, coumestrol, biochanin A measurement in blood and urine of different populations in relation to the intake of food (soy products) [ 91 , 92 , 93 , 94 ]. To be able to discriminate between these various isoflavonoids of interest that show chemical similarity (see Figure 5 ) the immunogens used for the production of antibodies should retain the moiety differing between the compounds exposed.…”

Section: Biological Methodsmentioning

confidence: 99%

Phenolics: Occurrence and Immunochemical Detection in Environment and Food

Meulenberg¹

2009

Molecules

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Phenolic compounds may be of natural or anthropogenic origin and be present in the environment as well as in food. They comprise a large and diverse group of compounds that may be either beneficial or harmful for consumers. In this review first a non-exhausting overview of interesting phenolics is given, in particular with regards to their presence in environment and food. For some of these compounds, beneficial, toxicological and/or optionally endocrine disrupting activities will be presented. Further, immunochemical detection and/or isolation methods developed will be discussed, including advantages and disadvantages thereof in comparison with conventional analytical methods such as HPLC, GC, MS. A short overview of new sensor-like methods will also be included for present and future application.

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Section: Biological Methodsmentioning

confidence: 99%

Phenolics: Occurrence and Immunochemical Detection in Environment and Food

Meulenberg¹

2009

Molecules

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Synthesis and application of organomercury haptens for enzyme-linked immunoassay of inorganic and organic mercury

Prudenté

Sirios

Cote

2010

Analytical Biochemistry

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Two organomercury haptens were synthesized via the classical oxymercuration reaction. An intramolecular oxymercuration reaction was the strategy employed to prepare a structurally simple, but chemically robust, organomercury hapten that was conjugated to chicken immunoglobulin G (IgG). The resulting immunogen afforded mouse anti-mercury antibodies that were evaluated in an enzyme-linked immunosorbent assay (ELISA). Antibodies demonstrating high titers were obtained, and various immunoassay parameters were investigated. The sensitivity and selectivity of the resulting antibodies were evaluated by exploring different cross-coupling chemistries and solid-phase synthetic variations. A second hapten was prepared with the intermolecular oxymercuration reaction, and the resulting compound, once coupled to carrier protein, afforded a solid-phase conjugate that revealed the versatility of the mouse anti-mercury antibody. The anti-mercury antibody developed in this study was capable of detecting both mercury(II) salts and organomercury compounds.

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