Synthesis of 2,5-furandicarboxylic acid by a TEMPO/laccase system coupled with Pseudomonas putida KT2440

Abstract

A novel biological approach for the production of FDCA by a TEMPO/laccase system coupled with Pseudomonas putida KT2440 was established.

Current Advances in the Sustainable Conversion of 5‐Hydroxymethylfurfural into 2,5‐Furandicarboxylic Acid

“…In general, different approaches were attempted to increase FDCA production by whole‐cell catalysis, such as parameter optimization (media conditions), [110,113,117,118] genetic engineering, [119–121] and the use of integrated strategy [101,122–124] …”

“…Several recent types of research employ a combination of enzymes and whole‐cell biocatalysts intending to match the advantages of the two methods [101,123,124] . For example, one‐pot synthesis of FDCA from HMF by utilizing the complementary catalytic activities of Comamonas testosteroni SC1588 cells and laccase/TEMPO system was reported by Yang et al [123] .…”

“…In general, different approaches were attempted to increase FDCA production by whole-cell catalysis, such as parameter optimization (media conditions), [110,113,117,118] genetic engineering, [119][120][121] and the use of integrated strategy. [101,[122][123][124] Regarding the genetic engineering approach, the highest FDCA production was obtained via the expression of two genes (HmfH and HMFO) in the R. ornithinolytica BF60 strain. [119] A tandem whole-cell cascade was reported by Tan et al [122] The combination of P. Putida and E. Coli led to full bioconversion of HMF to FDCA with a bioconversion rate much faster than other reported biocatalysts.…”

“…Several recent types of research employ a combination of enzymes and whole-cell biocatalysts intending to match the advantages of the two methods. [101,123,124] For example, one-pot synthesis of FDCA from HMF by utilizing the complementary catalytic activities of Comamonas testosteroni SC1588 cells and laccase/TEMPO system was reported by Yang et al [123] The new chemo-enzymatic approach to FDCA production was developed and the problematic TEMPO toxicity towards microbial cells that was described can be solved by continuous-flow technology and solvent engineering. [141] Zou et al [124] used TEMPO/ laccase system coupled with Pseudomonas Putida KT2440 to obtain 100 % HMF conversion and selectivity of FDCA under mild conditions, without complex gene modification, enzyme purification, or expensive cofactor addition.…”

Prospects of Using Biocatalysis for the Synthesis and Modification of Polymers

“…TEMPO/laccase afforded the selective oxidation of the hydroxymethyl group of HMF to form 5-formyl-2-furancarboxylic acid as a major product, which was subsequently oxidized to FDCA by P. putida KT2440. Manipulating the reaction conditions resulted in a good conversion of HMF (100%) and an excellent selectivity of FDCA (100%) at substrate concentrations up to 150 mM within 50 h [ 253 ]. The cascade catalytic process established in this and previous works offers an ecologically attractive approach to produce FDCA.…”