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Molecular imprinting represents one of the most promising strategies to design artificial enzyme inhibitors. However,t he study of molecularly imprinted enzyme inhibitors (MIEIs) remains at ap rimary stage.A dvanced applications of MIEIs for cell regulation have rarely been explored. Using asolid-phase oriented imprinting strategy so as to leave the active site of the enzymes accessible,w es ynthesized two MIEIs that exhibit high specificity and potent inhibitory effects (inhibition constant at low nM range) towardst rypsin and angiogenin. The trypsin MIEI inhibits trypsin activity,t ryptic digestion-induced extracellular matrix lysis and cell membrane destruction, indicating its utility in the treatment of active trypsin-dependent cell injury.T he angiogenin MIEI blocks cancer cell proliferation by suppressing the ribonuclease activity of angiogenin and decreasing the angiogenin level inside and outside HeLa cells.O ur work demonstrates the versatility of MIEIs for both enzyme inhibition and cell fate manipulation, showing their great potential as therapeutic drugs in biomedicine.

Abstract1,2-Propanediol is an important building block as a component used in the manufacture of unsaturated polyester resin, antifreeze, biofuel, nonionic detergent, etc. Commercial production of 1,2-propanediol through microbial biosynthesis is limited by low efficiency, and chemical production of 1,2-propanediol requires petrochemically derived routes involving wasteful power consumption and high pollution emissions. With the development of various strategies based on metabolic engineering, a series of obstacles are expected to be overcome. This review provides an extensive overview of the progress in the microbial production of 1,2-propanediol, particularly the different micro-organisms used for 1,2-propanediol biosynthesis and microbial production pathways. In addition, outstanding challenges associated with microbial biosynthesis and feasible metabolic engineering strategies, as well as perspectives on the future microbial production of 1,2-propanediol, are discussed.

A novel biological approach for the production of FDCA by a TEMPO/laccase system coupled with Pseudomonas putida KT2440 was established.