Synthesis, Characterization and Lipophilicity Study of Brucella abortus’ Immunogenic Peptide Sequence That Can Be Used in the Future Vaccination Studies

Acar, Tayfun; Arayıcı, Pelin Pelit; Uçar, Burcu; Karahan, Mesut; Mustafaeva, Zeynep

doi:10.1007/s10989-018-9739-0

Cited by 11 publications

(10 citation statements)

References 33 publications

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“…Based on our experience in previous similar studies, this purity is sufficient for the development of a vaccine prototype. Likewise, in the study of Moyle et al., vaccination studies were carried out with using similar purity peptides . Additionally, with the microwave‐assisted SPPS, it was obtained peptide with higher purity and chromatography peaks with high‐resolution indicating the high reaction yield.…”

Section: Resultsmentioning

confidence: 99%

Rabies Virus 31D Peptide‐[P(VP‐co‐AA)] Conjugates: Synthesis, Characterization and Cytotoxicity Evaluation

et al. 2019

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The prevention of rabies disease, which has been the subject of many pieces of researches since ancient times, has become possible by vaccination. Therefore, new generation vaccine systems should be developed to achieve more effective, accessible and reliable vaccines than conventional vaccines against this disease. In this study, modificated Rabies virus 31D antigenic peptide epitope was synthesized by microwave supported solid phase peptide synthesis method and the synthesized peptide was characterized. Bioconjugates of the antigenic peptide epitope with poly (N‐Vinyl‐2‐pyrrolidone‐co‐acrylic acid) that a biocompatible polymer was synthesized synthetically were synthesized at different ratios in the presence of 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide. Characterization of bioconjugates was performed with Gel Permeation Chromatography and ZetaSizer. Cytotoxicity test was carried out using ECV304 human epithelial cells to determine the biocompatibility of peptide, polymer and bioconjugates. It is thought that the produced biocompatible synthetic peptide‐polymer based rabies vaccine systems will contribute greatly to the vaccination studies.

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Section: Resultsmentioning

confidence: 99%

Rabies Virus 31D Peptide‐[P(VP‐co‐AA)] Conjugates: Synthesis, Characterization and Cytotoxicity Evaluation

et al. 2019

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“…It has validation since the chromatographic method has positive properties in terms of linearity, accuracy, precision and repeatability. Synthetic peptide vaccines are immunogens that can be used when creating vaccine prototypes, especially because of their lipophilic structure (which also allows cell permeability to pass easily) [43]. Methods such as Fourier transform infrared (FT-IR) and nuclear magnetic resonance (1H-and 31P-NMR) are frequently used to visualize the physical structure of the copolymer and peptide biopolymer conjugates and to perform characterization studies.…”

Section: Peptide Characterizationmentioning

confidence: 99%

New Generation Peptide-Based Vaccine Prototype

Özcan¹,

Karahan²,

Kumar³

et al. 2020

Current and Future Aspects of Nanomedicine

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Synthetic peptide-based vaccine prototypes are the future potential vaccination. Antigens, which belong to minimal microbial component and produce antibodies such as peptides and polysaccharides, can promote long-term protection against pathogens that can cause infectious diseases. Production of peptides becomes simple with solid phase peptide synthesis and microwave-assisted solid phase peptide synthesis using automatic synthesizers. The use of synthetic peptides was approved by the health authorities for vaccine design. Peptides are themselves very weak immunogens and need adjuvants to provide an effective autoimmune response. For this reason, peptide antigens are conjugated with biopolymers and loaded with nanoparticles. The toxicity of vaccine prototypes is evaluated in cell culture, and non-toxic prototypes are selected for vaccinating experimental animals. The most effective peptide-based vaccine prototype is determined as the one with the highest antibody level. The goal of this book chapter is to illustrate the use of peptides vaccine systems and present their opportunities with their future development.

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“…For example, when there is one or more Trp in the peptide sequence, using EDT in the cleavage will substantially protect Trp against oxidation. Typically, the peptide-bound resin is treated using 95% TFA with gentle shaking for 1-3 h [12,22,34,35].…”

Section: Solid-phase Peptide Synthesis (Ssps)mentioning

confidence: 99%

“…If purification of the synthesized peptide is required according to the application to be used, preparative HPLC is used. For the analysis, the peptide should be dissolved in a minimum volume of 0.1% TFA in water, and the acetonitrile and water gradient elution is adjusted according to the polarity of the peptide [12,22,[35][36][37]. The molecular weight of the synthesized peptide is confirmed by mass spectroscopy.…”

Section: Solid-phase Peptide Synthesis (Ssps)mentioning

confidence: 99%

“…It is also found in some systems that determine the amino acid sequence of the peptide molecule like amino acid analyzer and LC-MS-MS. Information on structural properties of peptides can also be obtained by NMR and FTIR methods. With NMR and FTIR, the structures of the peptides are further elucidated via specific binding and functional groups [12,35]. Moreover circular dichroism (CD) spectroscopy gives information about the conformation and secondary structures of polypeptides.…”

Section: Solid-phase Peptide Synthesis (Ssps)mentioning

confidence: 99%

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Synthesis and Applications of Synthetic Peptides

Uçar¹,

Acar²,

Arayıcı³

et al. 2019

Peptide Synthesis

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The synthesis and applications of the peptides are gaining increasing popularity as a result of the developments in biotechnology and bioengineering areas and for a number of research purposes including cancer diagnosis and treatment, antibiotic drug development, epitope mapping, production of antibodies, and vaccine design. The use of synthetic peptides approved by the health authorities for vaccine, for cancer, and in drug delivery systems is increasing with these developments. The aim of this book chapter is to review the recent developments in the use of peptides in the diagnosis of drug and vaccine systems and to present them to the reader with commercially available illustrations.

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Synthesis, Characterization and Lipophilicity Study of Brucella abortus’ Immunogenic Peptide Sequence That Can Be Used in the Future Vaccination Studies

Cited by 11 publications

References 33 publications

Rabies Virus 31D Peptide‐[P(VP‐co‐AA)] Conjugates: Synthesis, Characterization and Cytotoxicity Evaluation

Rabies Virus 31D Peptide‐[P(VP‐co‐AA)] Conjugates: Synthesis, Characterization and Cytotoxicity Evaluation

New Generation Peptide-Based Vaccine Prototype

Synthesis and Applications of Synthetic Peptides

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