Synthesis, characterization and applications of endophytic fungal nanoparticles

Ranjani, S; M, Shariq Ahmed; Adnan, Mohd; N, Senthil Kumar; Ruckmani, K.; Hemalatha, S.

doi:10.1080/24701556.2020.1784231

Cited by 40 publications

(5 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It helps the nanocolloids to penetrate into the cell by disturbing the cell membrane. This shape plays an important role in imparting efficient and effective antimicrobial activity through targeting the microbial cells through several mechanisms [21][22][23][24][25][26].…”

Section: Discussionmentioning

confidence: 99%

“…Then, the purified and concentrated nanocolloidal was suspended in 30% Dimethyl sulfoxide (DMSO) and ultrasonicated in ice for about 10 min. The polyherbal nanocolloidal solution was stored in room temperature for further use [21][22][23][24][25][26].…”

Section: Formulation Of Polyherbal Nanocolloids (Phnc)mentioning

confidence: 99%

“…FE-SEM analysis was used to analyze the shape and surface of the nanoparticles. EDAX (SIGMA HV-Carl Zeiss with Bruker Quantax 200-Z10 EDS Detector) analysis was carried out to detect the elemental composition present in the polyherbal nanocolloids (PHNc) [21][22][23][24][25][26][27].…”

Section: Physicochemical Characterization Of Polyherbal Nanocolloids (Phnc)mentioning

confidence: 99%

See 2 more Smart Citations

Novel Polyherbal Nanocolloids to Control Bovine Mastitis

Ranjani

Priya

Veerasami

et al. 2021

Appl Biochem Biotechnol

View full text Add to dashboard Cite

Mastitis is a widespread disease in dairy cattle occurring throughout the world. The increased use of antibiotics brings about the development of antibiotic-resistant microbes. The application of antibiotics in dairy farming led to increased antibiotic resistance and represents a major obstacle for the treatment of mastitis. Recent advancements in nanotechnology led to the development of nanocolloids to overcome disadvantages posed by conventional antimicrobial agents. Hence, a novel, environmentally friendly, cost-effective, biocompatible, and long-term antibacterial represents a promising solution for medicine and farming. Hence, polyherbal nanocolloids (PHNc) was formulated by using the extracts of Syzygium aromaticum, Cinnamomum verum, Emblica officinalis, Terminalia belerica, Terminalia chebula, and Cymbopogon citratus and physicochemically characterized. From mastitis milk samples, microorganisms were isolated including Acinetobacter junii, Klebsiella pneumoniae, Pseudomonas stutzeri, and Acinetobacter baumannii and screened for antibiotic susceptibility. All the isolated strains were tested with PHNc and compared with antibiotics. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and biofilm assays were performed at different concentrations, and antibacterial effects were quantified. In our results, PHNc showed potent bacteriostatic, bactericidal, and antibiofilm activity against all the strains. Our results indicated that PHNc can reduce the virulence factors responsible for infection by different bacterial strains. This study confirmed that PHNc had the potential to inhibit the growth of pathogenic Gram-negative and Gram-positive strains and could be utilized as an alternative to antibiotics to inhibit multidrug-resistant microbial pathogens in cattle.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Formulation Of Polyherbal Nanocolloids (Phnc)mentioning

confidence: 99%

See 1 more Smart Citation

Novel Polyherbal Nanocolloids to Control Bovine Mastitis

Ranjani

Priya

Veerasami

et al. 2021

Appl Biochem Biotechnol

View full text Add to dashboard Cite

show abstract

“…OSIP1 was attributed to the smaller size of Ag NPs obtained. Ranjani et al [98] used the same Ag NP biosynthesis strategy to inhibit the growth of P. aeruginosa ATCC 27853. Using the cell extract of the fungus L. theobromae (MK942601), it was possible to obtain agglomerated Ag particles with an average size of 163.3 nm.…”

Section: Metal-based Nanoparticles (Nps) Synthesized From Endophytic ...mentioning

confidence: 99%

Development of Antibiofilm Substances by Endophytic Microorganisms with an Emphasis on Medicine

Rodrigues¹,

Assis²,

Foggi³

et al. 2022

Focus on Bacterial Biofilms

View full text Add to dashboard Cite

The growing antimicrobial resistance and persistence of pathogenic microorganisms in infections–particularly in nosocomial infections–have become a major problem for public health worldwide. One of the main causes of these issues is the formation of biofilms, which are microbial communities associated with extracellular polymeric substances (EPS) that form a slimy extracellular matrix, causing the bacteria to become more tolerant to usual drugs in these structures. Thus, the search for new antibiofilm compounds is part of a strategy to deal with this problem. Endophytic microorganisms such as bacteria and fungi, mutualistically associated with plants, are sources of compounds with biological properties, including antimicrobials, and can be important allies in the synthesis of antibiofilm. These secondary metabolites can interfere with cell-to-cell communication and cell adhesion ability, promoting the dispersal of bacterial colonies and affecting biofilm. Since endophytes are cultivable in laboratory conditions, these microorganisms are environmentally friendly, as they do not contribute to pollution, are easy to handle and are produced on a large scale. Furthermore, metabolites from endophytes are of natural origin and may contribute to the reduced use of synthetic drugs. Considering these aspects, this chapter will focus on the characterization of endophytic microorganisms as potential active sources of antibiofilm and antimicrobial compounds with applications in medicine.

show abstract

“…Template DNA was ampli ed with a speci c set of primers for CTX-M-15 and AmpC gene as per the reaction condition adopted [31]. The PCR product was run using agarose gel electrophoresis to validate the presence of genes upon treatment with MBNc and Ampicillin when compared with the control [32].…”

Section: Analysis Of Ctx-m-15 and Ampc Gene Upon Mbnc Treatmentmentioning

confidence: 99%

Myrobalans Mediated Nanocolloids in Controlling Marine Pathogens

Ranjani

Parthasarathy

Rameshkumar

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Aquaculture production is affected by disease outbreak, which affect the production, profitability, and sustainability of the global aquaculture industry. Antibiotics have been widely used to control various pathogens. Due to its uncontrollable and indiscriminate usage, pathogens have developed resistance towards antibiotics. This current study aims to synthesize green silver- of silver nanocolloids (MBNc) from the extract of three myrobalans. MBNc was characterized by using UV-Vis spectroscopy, DLS, Zeta potential, FE-SEM. EDX, HRTEM, and SAED techniques. The array of antimicrobial assays were performed to evaluate the efficacy of MBNc against vibriosis causing pathogens including V. harveyi, V. alginolyticus, V. parahaemolyticus, and foodborne pathogen S. haemolyticus, isolated from infected fish. Further, the presence of ESBL genes including CTX-M-15 and Amp C were analyzed in control and MBNc treated strains. From our studies, it was observed that MBNc was very effective in controlling the growth and biofilm formation in all tested marine pathogens and effectively abolish the genes encoding ESBL CTX-M-15 in antibiotic-resistant pathogens tested. Thus, MBNc can be formulated to control the growth of marine pathogens and it can be used as an alternative to antibiotics to prevent infection in cage culturing and aquafarming.

show abstract

Synthesis, characterization and applications of endophytic fungal nanoparticles

Cited by 40 publications

References 22 publications

Novel Polyherbal Nanocolloids to Control Bovine Mastitis

Novel Polyherbal Nanocolloids to Control Bovine Mastitis

Development of Antibiofilm Substances by Endophytic Microorganisms with an Emphasis on Medicine

Myrobalans Mediated Nanocolloids in Controlling Marine Pathogens

Contact Info

Product

Resources

About