Synthesis and UV Curing Properties of A Photo-active Cardanol Derivative

Cheng, Chuanjie; ZHA, Jiwei; LIU, Zhongbin; Shen, Liang; Sun, Jian; Liu, Ying-Jia

doi:10.3724/sp.j.1095.2012.00254

Cited by 5 publications

(1 citation statement)

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“…Therefore, it is essential for growth and development of silkworm [1], and also the important object of silk biological, physiological and biochemical research in silkworm. As the achievement of whole genomic analysis of silkworm, some reports on the functional gene expressions of silkworm fat body have been published [2][3][4][5][6]. In this paper, differential proteome of fat body in different pupation phases was analyzed by two-dimensional electrophoresis (2-DE) and mass spectrum, which provide a new experimental basis for further investigation of physiological function and gene expression of fat body in silkworm.…”

Section: Introductionmentioning

confidence: 99%

Differential Expression Analysis of Fat Body Proteome during Pupation in Silkworm (<i>Bombyx mori</i>)

Wang¹,

Wu²,

Wang³

et al. 2011

AMR

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In order to investigate the differential expression of proteins related to pupation in silkworm, the fat body proteins were extracted from larvae of 5th day of 5th instar, un-pupated larvae and pupae of pupating day of multivoltine variety “da zao”. The optimized IPG-IEF/SDS-PAGE (2-DE) was used to analyze the differential expression of proteome in different developmental stage during pupation. The results showed that 66 proteins exhibited a more than two-fold differential expression, in which 43 proteins were down-regulated while 23 proteins were up-regulated. Additionally, 14 proteins with stage-specific expression characteristics were also observed. From 25 proteins with three-fold differential expression, 12 proteins were identified by MALDI-TOF/TOF MS, such as actin, calponin-like protein, NADH, beta-tubulin, receptor for activated protein kinase C, IMP cyclohydrolase, tropomyosin, antichymotrypsin precursor and 30K protein precursor etc. These results suggest that the differentially expressed proteins (enzymes) are related to the regulation of silkworm metamorphosis or sex differentiation, which provides a new experimental basis for better understanding the physiological functions and the mechanism of gene expression in silkworm fat body.

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