Abstract:In order to investigate the differential expression of proteins related to pupation in silkworm, the fat body proteins were extracted from larvae of 5th day of 5th instar, un-pupated larvae and pupae of pupating day of multivoltine variety “da zao”. The optimized IPG-IEF/SDS-PAGE (2-DE) was used to analyze the differential expression of proteome in different developmental stage during pupation. The results showed that 66 proteins exhibited a more than two-fold differential expression, in which 43 proteins were… Show more
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