Synthesis and characterizations of natural limestone-derived nano-hydroxyapatite (HAp): a comparison study of different metals doped HAps on antibacterial activity

Sinulingga, Karya; Sirait, Makmur; Siregar, Nurdin; Abdullah, Hairus

doi:10.1039/d1ra00308a

Cited by 30 publications

(23 citation statements)

References 51 publications

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“…Those peaks located at 38.4 and 44.5° could be attributed to the presence of Ag metal, while the other peaks at 31.3 and 33.6° could be assigned to the presence of the beta calcium triphosphate (β-TCP) phase. This phenomenon occurred due to the reduction of Ag ions under a high-temperature process, as we have comprehensively explained in our previous report . Interestingly, when the concentration of Ag is reduced to 2.5%, the X-ray diffraction remains identical to that of 5Ag-doped HAp, but there are no peaks at 38.4 and 44.5° for 2θ.…”

Section: Resultssupporting

confidence: 65%

“…Pristine HAp and Ag-doped HAp were prepared by similar procedures to our previous report . 2.5Ag/2.5Zn co-doped HAp and 2.5Ag/2.5Mg co-doped HAp were also synthesized by a similar method.…”

Section: Methodsmentioning

confidence: 97%

“…Pristine HAp and Ag-doped HAp were prepared by similar procedures to our previous report. 17 2.5Ag/2.5Zn co-doped HAp and 2.5Ag/2.5Mg co-doped HAp were also synthesized by a similar method. First, 5.6 g of CaO obtained by heating limestone (CaCO 3 ) at 900 °C for 5 h was added into 500 mL of deionized (DI) water under constant stirring.…”

Section: Methodsmentioning

confidence: 99%

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Investigation of Antibacterial Activity and Cell Viability of Ag/Mg and Ag/Zn Co-doped Hydroxyapatite Derived from Natural Limestone

et al. 2021

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Improving the antibacterial activity to avoid infections and keeping the biocompatibility at a safe level of HAp-based materials is highly important for biomedical applications. In this work, we investigate the antibacterial activity of 2.5Ag/2.5Mg co-doped HAp and 2.5Ag/2.5Zn co-doped HAp toward Escherichia coli bacteria. Moreover, their biocompatibility for osteoblastic cells (MC3T3-E1 cells) was also evaluated. The physical properties were characterized with necessary characterization tools such as X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and Brunauer–Emmett–Teller. Both 2.5Ag/2.5Mg and 2.5Ag/2.5Zn co-doped HAp consist of hydroxyapatite (HAp) and beta calcium triphosphate (β-TCP) phases. The antibacterial test reveals that 2.5Ag/2.5Mg co-doped HAp or 2.5Ag/2.5Zn co-doped HAp has an outstanding antibacterial activity with a killing rate of 99 ± 1%. More importantly, the cell viability for osteoblast cells with 2.5Ag/2.5Mg and 2.5Ag/2.5Zn co-doped HAp promotes the proliferation much more effectively than 2.5Ag-doped HAp or 5Ag-doped HAp.

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Section: Resultssupporting

confidence: 65%

“…Pristine HAp and Ag-doped HAp were prepared by similar procedures to our previous report . 2.5Ag/2.5Zn co-doped HAp and 2.5Ag/2.5Mg co-doped HAp were also synthesized by a similar method.…”

Section: Methodsmentioning

confidence: 97%

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Investigation of Antibacterial Activity and Cell Viability of Ag/Mg and Ag/Zn Co-doped Hydroxyapatite Derived from Natural Limestone

et al. 2021

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“…1d-i). CaAlg and HAP began to form as soon as calci ed, and the crystallinity of HAP might be con rmed after 10 min (Sinulingga et al 2021). CaAlg exhibited a smooth layered microstructure (Fig.…”

Section: Resultsmentioning

confidence: 99%

Simple and green synthesis of calcium alginate/hydroxyapatite hybrid material without high temperature treatment and its flame retardancy

et al. 2022

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Aiming to improve the thermal stability and ame retardant properties of calcium alginate, calcium alginate (CaAlg)/hydroxyapatite (HAP) hybrid material was synthesized in situ by the sol-gel method at room temperature. It was found that the HAP particles were generated from nanospheres to urchin-like microspheres, nally to wrinkled sponge microspheres with the two-dimensional nanostructured surface. The results show that the thermal stability and ame retardancy of the hybrid material were signi cantly improved, and its mechanism was proposed as HAP promoted the decarboxylation of alginate resulting in carbonization of CaAlg/HAP, and it was the function of soild phase before 350 ℃, while the synergistic effects of gas phase and solid phase after 350 ℃ that led to the high ame retardancy of CaAlg/HAP.

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“…But all samples were above the grade limit of 70%, which means the sample had a good percentage of live cells. The percentage of living cells produced from the MTT test proved that HAp compounds were not toxic to fibroblast cells [16].…”

Section: Cytotoxicity Testmentioning

confidence: 93%

Characterization of hydroxyapatite by cytotoxicity test and bending test

Sirait

Sinulingga

Siregar

et al. 2022

J. Phys.: Conf. Ser.

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Hydroxyapatite has been synthesized as a bone graft material by analyzing the membrane made according to the cytotoxicity test and bending test. The method used is dry ball milling. Cytotoxicity testing was carried out by making isolated osteoblast cells cultured in a 75 cm2 cell flask containing a minimum of essential media. Based on the cytotoxicity test, it was found that hydroxyapatite is not toxic with cell viability above 60% (non-toxic requirements ISO 10993-5). From the variation of the hydroxyapatite mixture used, the average cell viability was (100.48 ± 11.98)%. A mixture of 2.5% Ag and 5% Ag with hydroxyapatite also provides good viability, which is above 60%, which means it is not toxic. The results of the analysis of the bending test and the compressive test showed that the larger the hydroxyapatite mixture, the lower the bending and compressive test results. The maximum bending test on a 10% mixture is 22.1 M.Pa and the compressive test on a 5% mixture is 16 °ShD.

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Synthesis and characterizations of natural limestone-derived nano-hydroxyapatite (HAp): a comparison study of different metals doped HAps on antibacterial activity

Abstract: Antibacterial activities of different metal ion doped HAp towards (a) E. coli and (b) S. aureus bacteria.

Cited by 30 publications

References 51 publications

Investigation of Antibacterial Activity and Cell Viability of Ag/Mg and Ag/Zn Co-doped Hydroxyapatite Derived from Natural Limestone

Investigation of Antibacterial Activity and Cell Viability of Ag/Mg and Ag/Zn Co-doped Hydroxyapatite Derived from Natural Limestone

Simple and green synthesis of calcium alginate/hydroxyapatite hybrid material without high temperature treatment and its flame retardancy

Characterization of hydroxyapatite by cytotoxicity test and bending test

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