Abstract:New water-based nanofluids including unparalleled milk protein α-lactalbumin hollow nano-bio-tubes using low cost, available and advanced partial chemical hydrolysis strategy in bottom-up nano-assembly have been employed in this work. The aqueous sol-gel chemistry in nanotechnology which we selected for this goal offers new fabrication as interesting smart protein nanotubes. The kinds of nanometer sized tubular structures such as waved, helically coiled, bent, bamboo-shaped, bead-like and branched single-walle… Show more
“…In addition, according to the obtained spectra, SWCNTs have a small amount of absorbed or covalent chemical additives. Our spectra were similar to those presented by other authors [ 18 , 19 ], which allowed us to conclude that compliance with the chemical structure of the samples; their proper degree of purification are relevant for use in further biological studies. Figure 2 FTIR spectra of SWCNTs.…”
Section: Resultssupporting
confidence: 88%
“…Therefore, the usage of SWCNTs as basis for intensive growth of cells in suspension, three-dimensional culture, and tissue-like structures seems very promising. Proof of this is in well-known biotechnology experiments which describe fibroblast-like and endothelial cells growth on thin films of SWCNTs [ 19 ]. Second, the trend influences the chemical properties of nanomaterials on the formation of MTS.…”
This paper describes the impact of different concentrations of single-walled carbon nanotubes (SWCNTs) on cell viability of breast adenocarcinoma, MCF-7 line, and formation of multicellular tumor spheroids (MTS). Chemical composition and purity of nanotubes is controlled by Fourier transform infrared spectroscopy. The strength and direction of the influence of SWCNTs on the tumor cell population was assessed by cell counting and measurement of the volume of multicellular tumor spheroids. Effect of SWCNTs on the formation of multicellular spheroids was compared with the results obtained by culturing tumor cells with ultra dispersed diamonds (UDDs). Our results demonstrated that SWCNTs at concentrations ranging from 12.5 to 50 μg/ml did not have cytotoxic influence on tumor cells; instead, they had weak cytostatic effect. The increasing of SWCNTs concentration to 100 to 200 μg/ml stimulated proliferation of tumor cells, especially in suspension fractions. The result of this influence was in formation of more MTS in cell culture with SWCNTs compared with UDDs and control samples. In result, the median volume of MTS after cultivation with SWCNTs at 100 to 200 μg/ml concentrations is 3 to 5 times greater than that in samples which were incubated with the UDDs and is 2.5 times greater than that in control cultures. So, if SWCNTs reduced cell adhesion to substrate and stimulated formation of tumor cell aggregates volume near 7 · 10−3 mm3, at the same time, UDDs reduced adhesion and cohesive ability of cells and stimulated generation of cell spheroids volume no more than 4 · 10−3 mm3. Our results could be useful for the control of cell growth in three-dimensional culture.PACS61. 46 + w; 61.48 + c; 61.48De; 87.15-v; 87.64-t
“…In addition, according to the obtained spectra, SWCNTs have a small amount of absorbed or covalent chemical additives. Our spectra were similar to those presented by other authors [ 18 , 19 ], which allowed us to conclude that compliance with the chemical structure of the samples; their proper degree of purification are relevant for use in further biological studies. Figure 2 FTIR spectra of SWCNTs.…”
Section: Resultssupporting
confidence: 88%
“…Therefore, the usage of SWCNTs as basis for intensive growth of cells in suspension, three-dimensional culture, and tissue-like structures seems very promising. Proof of this is in well-known biotechnology experiments which describe fibroblast-like and endothelial cells growth on thin films of SWCNTs [ 19 ]. Second, the trend influences the chemical properties of nanomaterials on the formation of MTS.…”
This paper describes the impact of different concentrations of single-walled carbon nanotubes (SWCNTs) on cell viability of breast adenocarcinoma, MCF-7 line, and formation of multicellular tumor spheroids (MTS). Chemical composition and purity of nanotubes is controlled by Fourier transform infrared spectroscopy. The strength and direction of the influence of SWCNTs on the tumor cell population was assessed by cell counting and measurement of the volume of multicellular tumor spheroids. Effect of SWCNTs on the formation of multicellular spheroids was compared with the results obtained by culturing tumor cells with ultra dispersed diamonds (UDDs). Our results demonstrated that SWCNTs at concentrations ranging from 12.5 to 50 μg/ml did not have cytotoxic influence on tumor cells; instead, they had weak cytostatic effect. The increasing of SWCNTs concentration to 100 to 200 μg/ml stimulated proliferation of tumor cells, especially in suspension fractions. The result of this influence was in formation of more MTS in cell culture with SWCNTs compared with UDDs and control samples. In result, the median volume of MTS after cultivation with SWCNTs at 100 to 200 μg/ml concentrations is 3 to 5 times greater than that in samples which were incubated with the UDDs and is 2.5 times greater than that in control cultures. So, if SWCNTs reduced cell adhesion to substrate and stimulated formation of tumor cell aggregates volume near 7 · 10−3 mm3, at the same time, UDDs reduced adhesion and cohesive ability of cells and stimulated generation of cell spheroids volume no more than 4 · 10−3 mm3. Our results could be useful for the control of cell growth in three-dimensional culture.PACS61. 46 + w; 61.48 + c; 61.48De; 87.15-v; 87.64-t
“…In addition, according to the obtained spectra, SWCNTs have a small amount of absorbed or covalent chemical additives. Our spectra were similar to those presented by other authors [18,19], which allowed us to conclude that compliance with the chemical structure of the samples; their proper degree of purification are relevant for use in further biological studies.…”
Section: Chemical Properties Of Nanomaterialssupporting
This paper describes the impact of different concentrations of single-walled carbon nanotubes (SWCNTs) on cell viability of breast adenocarcinoma, MCF-7 line, and formation of multicellular tumor spheroids (MTS). Chemical composition and purity of nanotubes is controlled by Fourier transform infrared spectroscopy. The strength and direction of the influence of SWCNTs on the tumor cell population was assessed by cell counting and measurement of the volume of multicellular tumor spheroids. Effect of SWCNTs on the formation of multicellular spheroids was compared with the results obtained by culturing tumor cells with ultra dispersed diamonds (UDDs). Our results demonstrated that SWCNTs at concentrations ranging from 12.5 to 50 μg/ml did not have cytotoxic influence on tumor cells; instead, they had weak cytostatic effect. The increasing of SWCNTs concentration to 100 to 200 μg/ml stimulated proliferation of tumor cells, especially in suspension fractions. The result of this influence was in formation of more MTS in cell culture with SWCNTs compared with UDDs and control samples. In result, the median volume of MTS after cultivation with SWCNTs at 100 to 200 μg/ml concentrations is 3 to 5 times greater than that in samples which were incubated with the UDDs and is 2.5 times greater than that in control cultures. So, if SWCNTs reduced cell adhesion to substrate and stimulated formation of tumor cell aggregates volume near 7 · 10 −3 mm 3 , at the same time, UDDs reduced adhesion and cohesive ability of cells and stimulated generation of cell spheroids volume no more than 4 · 10 −3 mm 3 . Our results could be useful for the control of cell growth in three-dimensional culture.
“…Mn 2+ , Zn 2+ , Ca 2+ and Fe 2+ ) or mixtures are also used to induce tubular self-assembly of partially hydrolyzed a-La and the molar ratio determined growth rate. Nanotubes obtained by this method had 3-8 nm in outer diameters (Esmaeilzadeh et al, 2013). Despite of the interesting properties of protein nanotubes and their intrinsic potential for use as carrier structures, only one published work describing the application of BSA nanotubes to incorporate curcumin as bioactive compound has appeared-see Table 2 (Sadeghi et al, 2013).…”
Whey proteins are widely used as nutritional and functional ingredients in formulated foods because they are relatively inexpensive, generally recognized as safe (GRAS) ingredient, and possess important biological, physical, and chemical functionalities. Denaturation and aggregation behavior of these proteins is of particular relevance toward manufacture of novel nanostructures with a number of potential uses. When these processes are properly engineered and controlled, whey proteins may be formed into nanohydrogels, nanofibrils, or nanotubes and be used as carrier of bioactive compounds. This review intends to discuss the latest understandings of nanoscale phenomena of whey protein denaturation and aggregation that may contribute for the design of protein nanostructures. Whey protein aggregation and gelation pathways under different processing and environmental conditions such as microwave heating, high voltage, and moderate electrical fields, high pressure, temperature, pH, and ionic strength were critically assessed. Moreover, several potential applications of nanohydrogels, nanofibrils, and nanotubes for controlled release of nutraceutical compounds (e.g. probiotics, vitamins, antioxidants, and peptides) were also included. Controlling the size of protein networks at nanoscale through application of different processing and environmental conditions can open perspectives for development of nanostructures with new or improved functionalities for incorporation and release of nutraceuticals in food matrices.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
