Synthesis and characterization of silver nanoparticles using fruit extract of Momordica cymbalaria and assessment of their in vitro antimicrobial, antioxidant and cytotoxicity activities

Swamy, Mallappa Kumara; Akhtar, Mohd Sayeed; Mohanty, Sudipta Kumar; Sinniah, Uma Rani

doi:10.1016/j.saa.2015.07.009

Cited by 130 publications

(61 citation statements)

References 23 publications

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“…The highest cell viability (100%) observed at 0 μg/mL began to decrease gradually with the increased concentrations and exposure time from 24 h to 72 h. The highest toxicity effect was observed at 500 μg/mL where 17% and 20% cell viability was observed after 72 h in CuFe2O4 and ZnFe2O4 nanoparticles, respectively, suggesting high toxicity at higher concentrations. Similarly, previous studies also have shown the dose dependent cytotoxicity of several types of nanoparticles [20,35,37,54,55]. Interestingly, both nanoparticles were observed to be less toxic at lower concentrations up to 100 μg/mL where about 80% of the cells were viable.…”

Section: Cytotoxicity Analysissupporting

confidence: 73%

“…We evaluated the cytotoxic effect of CuFe 2 O 4 and ZnFe 2 O 4 nanoparticles on the human breast cancer cell line (MCF-7) as described previously by Swamy et al [35]. Briefly, the cells were cultured on DMEM (Dulbecco's Modified Eagles Medium) (Sigma-Aldrich, St. Louis, MO, USA) added with L-glutamine (2 mM) (PAA Laboratories GmbH, Pasching, Austria), penicillin (100 U/mL) (Sigma-Aldrich, St. Louis, MO, USA), streptomycin (100 µg/mL) (Sigma-Aldrich, St. Louis, MO, USA) and fetal bovine serum (10%) (PAA Laboratories GmbH, Pasching, Austria).…”

Section: Evaluation Of Cytotoxicity Activitymentioning

confidence: 99%

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Evaluation of Antioxidant and Cytotoxicity Activities of Copper Ferrite (CuFe2O4) and Zinc Ferrite (ZnFe2O4) Nanoparticles Synthesized by Sol-Gel Self-Combustion Method

et al. 2016

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Spinel copper ferrite (CuFe 2 O 4 ) and zinc ferrite (ZnFe 2 O 4 ) nanoparticles were synthesized using a sol-gel self-combustion technique. The structural, functional, morphological and magnetic properties of the samples were investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and vibrating sample magnetometry (VSM). XRD patterns conform to the copper ferrite and zinc ferrite formation, and the average particle sizes were calculated by using a transmission electron microscope, the measured particle sizes being 56 nm for CuFe 2 O 4 and 68 nm for ZnFe 2 O 4. Both spinel ferrite nanoparticles exhibit ferromagnetic behavior with saturation magnetization of 31 emug´1 for copper ferrite (50.63 Am 2 /Kg) and 28.8 Am 2 /Kg for zinc ferrite. Both synthesized ferrite nanoparticles were equally effective in scavenging 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) free radicals. ZnFe 2 O 4 and CuFe 2 O 4 nanoparticles showed 30.57%˘1.0% and 28.69%˘1.14% scavenging activity at 125 µg/mL concentrations. In vitro cytotoxicity study revealed higher concentrations (>125 µg/mL) of ZnFe 2 O 4 and CuFe 2 O 4 with increased toxicity against MCF-7 cells, but were found to be non-toxic at lower concentrations suggesting their biocompatibility.

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Section: Cytotoxicity Analysissupporting

confidence: 73%

Section: Evaluation Of Cytotoxicity Activitymentioning

confidence: 99%

Evaluation of Antioxidant and Cytotoxicity Activities of Copper Ferrite (CuFe2O4) and Zinc Ferrite (ZnFe2O4) Nanoparticles Synthesized by Sol-Gel Self-Combustion Method

et al. 2016

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“…Bacterial strains were grown in Mueller-Hinton agar plates, whereas fungal strains were grown in Potato Dextrose agar plates. A set of three concentrations (25,50 and 100 µg/ml of MnFe 2 O 4 nanoparticles) were used in this study. Gentamycin was used as positive control for bacteria and Ketaconozole was used as positive control for fungus.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

“…Thus, investigation of new chemicals for treatment of cancer is necessary. Various types of nanoparticles synthesized from inorganic as well as organic materials shows potential applications in cancer therapy [24,25]. The remarkable heating effects of magnetic nanoparticles used as drug delivery structures, provide an opportunity to target tumor cells [26].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and comparative studies of MnFe2O4 nanoparticles with different natural polymers by sol–gel method: structural, morphological, optical, magnetic, catalytic and biological activities

Jacintha

Umapathy

Neeraja³

et al. 2017

J Nanostruct Chem

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Nanosized manganese ferrite (MnFe 2 O 4 ) particles were prepared by sol-gel method using natural polymers like wheat flour (WF) and potato flour (PF) as surfactants and its structural, morphological, optical and magnetic characteristics were studied by X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FT-IR), scanning electron microscope (SEM), photoluminescence spectroscopy (PL) and vibration sample magnetometer (VSM). Brunauer-Emmett-Teller (BET) surface area test also performed and the results obtained were discussed. The average crystallite size was found to be 23 and 16 nm for WF/MnFe 2 O 4 and PF/MnFe 2 O 4 samples, respectively. Magnetic hysteresis loops confirmed the super-paramagnetic behavior for both the samples. For oxidation of benzyl alcohol to benzaldehyde, the catalytic activity of MnFe 2 O 4 nanoparticles (NPs) was carried out. Antimicrobial and antifungal activity of WF/MnFe 2 O 4 and PF/MnFe 2 O 4 samples were investigated against two Gram-positive bacteria (Staphylococcus aureus, Streptococcus pneumoniae), two Gram-negative bacteria (Pseudomonas aeruginosa, Salmonella paratyphi) and fungus (Candida albicans) using inhibition zone method. Minimum Inhibitory Concentration (MIC) values also calculated to determine susceptibilities of bacteria to drugs and also to evaluate the activity of new antimicrobial agents. The in vitro cytotoxicity of newly synthesized samples were analyzed by MTT assay against MCF-7, A549 and HaCaT cell lines in a dose-dependent fashion. Among these two samples, sample B (using potato flour) shows better response than sample A (using wheat flour) and both the samples were non-toxic to normal cell line. The concentration required to kill 50% of the cell (IC 50 ) was also calculated. Graphical AbstractMnFe 2 O 4 nanoparticles were synthesized by sol-gel method using natural polymers, wheat flour and potato flour, as surfactant. The as-prepared MnFe 2 O 4 was characterized by XRD, FT-IR, SEM, EDX, PL and VSM analysis. The average crystallite size was found to be 23 and 16 nm for WF/MnFe 2 O 4 and PF/MnFe 2 O 4 samples, respectively. Magnetic hysteresis loops confirmed the super-paramagnetic behavior for both the samples. The catalytic activity of MnFe 2 O 4 nanoparticles (NPs) were carried out for oxidation of benzyl alcohol to benzaldehyde. Biological activities like antimicrobial, antifungal and anticancer activities of the samples were investigated. Among these two samples, PF/MnFe 2 O 4 shows better response than WF/MnFe 2 O 4 and both the samples were non-toxic to normal cell line.

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“…Researchers [19][20][21]23] have dedicated their efforts to the synthesis and study of spinel ferrites due to the special properties they exhibit in the nano range. In recent years, various types of nanoparticles synthesized from inorganic as well as organic materials have shown potential applications in cancer therapy [24,25]. Magnetic nanoparticles used as drug delivery structures appear very beneficial as they show remarkable heating effects and thus provide an opportunity to target tumor cells specifically [26,27].…”

Section: Fourier Transform Infrared Spectroscopymentioning

confidence: 99%

Synthesis, Characterization and in Vitro Evaluation of Manganese Ferrite (MnFe2O4) Nanoparticles for Their Biocompatibility with Murine Breast Cancer Cells (4T1)

et al. 2016

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Manganese ferrite (MnFe 2 O 4 ) magnetic nanoparticles were successfully prepared by a sol-gel self-combustion technique using iron nitrate and manganese nitrate, followed by calcination at 150˝C for 24 h. Calcined sample was systematically characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and vibrational sample magnetometry (VSM) in order to identify the crystalline phase, functional group, morphology, particle size, shape and magnetic behavior. It was observed that the resultant spinal ferrites obtained at low temperature exhibit single phase, nanoparticle size and good magnetic behavior. The study results have revealed the existence of a potent dose dependent cytotoxic effect of MnFe 2 O 4 nanoparticles against 4T1 cell lines at varying concentrations with IC 50 values of 210, 198 and 171 µg/mL after 24 h, 48 h and 72 h of incubation, respectively. Cells exposed to higher concentrations of nanoparticles showed a progressive increase of apoptotic and necrotic activity. Below 125 µg/mL concentration the nanoparticles were biocompatible with 4T1 cells.

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Synthesis and characterization of silver nanoparticles using fruit extract of Momordica cymbalaria and assessment of their in vitro antimicrobial, antioxidant and cytotoxicity activities

Cited by 130 publications

References 23 publications

Evaluation of Antioxidant and Cytotoxicity Activities of Copper Ferrite (CuFe2O4) and Zinc Ferrite (ZnFe2O4) Nanoparticles Synthesized by Sol-Gel Self-Combustion Method

Evaluation of Antioxidant and Cytotoxicity Activities of Copper Ferrite (CuFe2O4) and Zinc Ferrite (ZnFe2O4) Nanoparticles Synthesized by Sol-Gel Self-Combustion Method

Synthesis and comparative studies of MnFe2O4 nanoparticles with different natural polymers by sol–gel method: structural, morphological, optical, magnetic, catalytic and biological activities

Synthesis, Characterization and in Vitro Evaluation of Manganese Ferrite (MnFe2O4) Nanoparticles for Their Biocompatibility with Murine Breast Cancer Cells (4T1)

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