Synthesis, Characterization and in Vitro Evaluation of Manganese Ferrite (MnFe2O4) Nanoparticles for Their Biocompatibility with Murine Breast Cancer Cells (4T1)

Kanagesan, S.; Aziz, Sidek Bin Ab; Hashim, Mansor; Ismail, Ismayadi; Subramani, Tamilselvan; Alitheen, Noorjahan Banu; Swamy, Mallappa Kumara; Rao, Bandaru Purna Chandra

doi:10.3390/molecules21030312

Cited by 61 publications

(26 citation statements)

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“…The malignant transformation of tumor mainly manifests as an accelerated proliferation of tumor cells, easy invasion and metastasis. The optimal treatment or operation opportunity of breast cancer, one of the frequent malignant tumors in females, is often lost due to tumor cell invasion, reduction in quality of life and shortening of the survival time of patients (10,11). Currently, the development of antitumor drugs mainly focuses on killing tumor cells and inhibiting tumor cell migration (12).…”

Section: Discussionmentioning

confidence: 99%

Effect of HMGN2 on proliferation and apoptosis of MCF-7 breast cancer cells

et al. 2018

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We investigated the effect of high mobility group protein N2 (HMGN2) on the proliferation and apoptosis of the human MCF-7 breast cancer cell line, and its effect on tumor growth in a subcutaneous heterotopic transplantation tumor model of breast cancer. The cell viability assay was used to verify the effect of the recombinant human HMGN2 on MCF-7 cell proliferation. The Transwell chamber assay was used to verify the effect of HMGN2 on MCF-7 cell migration. Flow cytometry and Hoechst staining were used to detect the effect of HMGN2 on MCF-7 cell apoptosis. MCF-7 was injected to establish a subcutaneous heterotopic transplantation tumor model of breast cancer in nude mice. The size, weight and volume of tumor in each group were compared after the administration of different concentrations of HMGN2 solution around the tumor tissue at day 1, 3, 5 and 7. The tumor tissue was removed and cut into sections, and the apoptotic cells in tumors of nude mice were detected by a TUNEL kit. The CCK-8 assay showed that HMGN2 at different concentrations inhibited the proliferation of the MCF-7 breast cancer cells, and the proliferation of MCF-7 cells were significantly inhibited when the concentration of HMGN2 reached 3 µg/ml (P<0.01). The Transwell chamber assay showed that 3 µg/ml of HMGN2 significantly decreased the migration capacity of MCF-7 cells (P<0.01). Flow cytometry and Hoechst staining showed that 3 µg/ml of HMGN2 significantly increased apoptosis of MCF-7 cells (P<0.01). After the nude mouse model of breast cancer was established, HMGN2 at different concentrations was injected around the tumor tissue at day 1, 3, 5 and 7. We demonstrated that the growth of breast cancer was significantly inhibited when the concentration of HMGN2 reached 15 µg/ml. TUNEL staining showed that the number of apoptotic cells in the 15 µg/ml dose group was significantly higher than that in the control group (P<0.01). Therefore, in vitro and in vivo experiments proved that recombinant human HMGN2 could significantly inhibit the proliferation and migration of breast cancer cells, which increased the apoptosis of breast cancer cells and exerted anti-breast cancer effects, which enriched our understanding of the biological roles of HMGN2.

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Section: Discussionmentioning

confidence: 99%

Effect of HMGN2 on proliferation and apoptosis of MCF-7 breast cancer cells

et al. 2018

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“…Various studies on the immune compatibility of NPs have been discussed in detail elsewhere [235]. More recently, 4T1 cells (a murine breast cancer cell line) exposed to below 125 µg/mL of manganese ferrite (MnFe 2 O 4 ) NPs showed biocompatibility [238]. According to Tomitaka et al [239], various ferrite NPs (Fe 3 O 4 , ZnFe 3 O 4 , and NiFe 3 O 4 ) above 100 μg/mL show toxicity against HeLa cell lines.…”

Section: Biological Compatibility Of Nanoparticles (Nps)mentioning

confidence: 99%

Nanoparticles: Alternatives Against Drug-Resistant Pathogenic Microbes

Rudramurthy¹,

et al. 2016

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Antimicrobial substances may be synthetic, semisynthetic, or of natural origin (i.e., from plants and animals). Antimicrobials are considered "miracle drugs" and can determine if an infected patient/animal recovers or dies. However, the misuse of antimicrobials has led to the development of multi-drug-resistant bacteria, which is one of the greatest challenges for healthcare practitioners and is a significant global threat. The major concern with the development of antimicrobial resistance is the spread of resistant organisms. The replacement of conventional antimicrobials by new technology to counteract antimicrobial resistance is ongoing. Nanotechnology-driven innovations provide hope for patients and practitioners in overcoming the problem of drug resistance. Nanomaterials have tremendous potential in both the medical and veterinary fields. Several nanostructures comprising metallic particles have been developed to counteract microbial pathogens. The effectiveness of nanoparticles (NPs) depends on the interaction between the microorganism and the NPs. The development of effective nanomaterials requires in-depth knowledge of the physicochemical properties of NPs and the biological aspects of microorganisms. However, the risks associated with using NPs in healthcare need to be addressed. The present review highlights the antimicrobial effects of various nanomaterials and their potential advantages, drawbacks, or side effects. In addition, this comprehensive information may be useful in the discovery of broad-spectrum antimicrobial drugs for use against multi-drug-resistant microbial pathogens in the near future.

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“…Typically, the metal oxide vibrations occur below 1000 cm À1 . 64,65 For spectrum of RhB, the weak intensity band at 2983 cm À1 is associated with the aromatic C-H vibrations, which also appear at 1130 cm À1 (plane bending) and at 682 cm À1 (wagging vibrations). Moreover, the vibration bands in 2928-2869 cm À1 is inferred to the presence of RhB.…”

Section: Fourier Transform Infrared Analysismentioning

confidence: 99%

Effect of a pH-controlled co-precipitation process on rhodamine B adsorption of MnFe₂O₄ nanoparticles

Lamdab

Wetchakun

2018

RSC Adv.

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Synthesis, Characterization and in Vitro Evaluation of Manganese Ferrite (MnFe2O4) Nanoparticles for Their Biocompatibility with Murine Breast Cancer Cells (4T1)

Cited by 61 publications

References 35 publications

Effect of HMGN2 on proliferation and apoptosis of MCF-7 breast cancer cells

Effect of HMGN2 on proliferation and apoptosis of MCF-7 breast cancer cells

Nanoparticles: Alternatives Against Drug-Resistant Pathogenic Microbes

Effect of a pH-controlled co-precipitation process on rhodamine B adsorption of MnFe₂O₄ nanoparticles

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Synthesis, Characterization and in Vitro Evaluation of Manganese Ferrite (MnFe2O4) Nanoparticles for Their Biocompatibility with Murine Breast Cancer Cells (4T1)

Cited by 61 publications

References 35 publications

Effect of HMGN2 on proliferation and apoptosis of MCF-7 breast cancer cells

Effect of HMGN2 on proliferation and apoptosis of MCF-7 breast cancer cells

Nanoparticles: Alternatives Against Drug-Resistant Pathogenic Microbes

Effect of a pH-controlled co-precipitation process on rhodamine B adsorption of MnFe2O4 nanoparticles

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Product

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Effect of a pH-controlled co-precipitation process on rhodamine B adsorption of MnFe₂O₄ nanoparticles