Synthesis and characterization of modified magnetic nanoparticles as theranostic agents: in vitro safety assessment in healthy cells

Prokopiou, E Danai; Pissas, M.; Fibbi, Gabriella; Margheri, Francesca; Kalska-Szostko, B.; Papanastasiou, Giorgos; Jansen, Maurits A.; Wang, Jansen; Laurenzana, Anna; Efthimiadou, K Eleni

doi:10.1016/j.tiv.2021.105094

Cited by 13 publications

(14 citation statements)

References 41 publications

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“…Further, the colloidal stability of IONPs-CIT and IONPs-TMAOH was confirmed by Zeta Potential. These results are in agreement with Prokopiou et al (2021) who synthesized iron oxide nanoparticles by the modified coprecipitation method using trisodium citrate, which through electrostatic interactions were a passive layer on the surface of the nanoparticles. In this study, the authors reported that the citrate-functionalized IONPs had a hydrodynamic diameter of 319±20.98 nm with PdI 0.55±0.09 and Zeta Potential of -41.1±0.46 mV (Prokopiou et al, 2021).…”

Section: Resultssupporting

confidence: 92%

“…If the feasibility is below 70%, it is understood that there is a cytotoxic potential (Standard, 2009). This results, inform that the nanoparticles did not show significant cytotoxicity, when in concentrations from 5 to 300 µg/mL, with an incubation time of up to 6 h. The biocompatibility and biosafety of the use of IONPs-CIT can also be confirmed in the findings of Prokopiou et al (2021) who performed a cytotoxicity study of iron oxide nanoparticles functionalized with trisodium citrate in human keratinocyte cells, NCTC2544 and in a cell line with epithelial morphology isolated from the kidney of a human embryo, HEK-293. In this work, concentrations of 1,10,25,50,100 and 200 μg/mL were studied, observing high values of mitochondrial activity in the NCTC2544 line and reduction of viable cells in the HEK-293 lineage when in high concentrations.…”

Section: Resultssupporting

confidence: 69%

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Síntese de nanopartículas de óxido de ferro estabilizadas com citrato de sódio e TMAOH

Cândido

Rost

Ferreira

et al. 2022

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As nanopartículas de óxido de ferro (IONPs) representam uma classe de nanomateriais magnéticos e biocompatíveis que têm sido amplamente utilizados em pesquisas e aplicações médicas, como estudos de hipertermia, como agentes de contraste para ressonância magnética, biossensores, entre outros. Entretanto, sua aplicação depende de fatores como, morfologia, tamanho e propriedade de superfície estejam ajustados. As IONPs podem ser obtidas por diferentes métodos de síntese, no entanto, a coprecipitação química representa uma rota de síntese mais simples, fácil e rápida, na qual soluções aquosas de precursores contendo íons ferro (Fe3+) e ferroso (Fe2+) são alcalinizadas sob controle de temperatura e pH. Este estudo propõe sintetizar nanopartículas de óxido de ferro pelo método de coprecipitação química e estabilizá-las com citrato de sódio (IONPs-CIT) e hidróxido de tetrametilamônio (IONPs-TMAOH). Além disso, caracterizar o diâmetro hidrodinâmico e o Potencial Zeta das amostras por espalhamento dinâmico da luz. A citotoxicidade das IONPs-CIT na linhagem celular MDA-MB-468 foi avaliada através da análise da atividade mitocondrial.

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Section: Resultssupporting

confidence: 92%

Section: Resultssupporting

confidence: 69%

Síntese de nanopartículas de óxido de ferro estabilizadas com citrato de sódio e TMAOH

Cândido

Rost

Ferreira

et al. 2022

RSD

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“…Besides having adequate biocompatibility and small nanoscale size, magnetic NPs provide a unique platform for theranostic uses [33]. However, one of the most critical challenges in employing magnetic NPs is their safety in healthy cells [34].…”

Section: Discussionmentioning

confidence: 99%

CoNi alloy nanoparticles for cancer theranostics: synthesis, physical characterization, in vitro and in vivo studies

et al. 2021

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Nanomaterials are attracting increasing interest in many biomedical fields, including the fight against cancer. In this context, we successfully synthesized CoNi alloy nanoparticles (NPs) by a simple polyol process. The magnetic characteristics of the products were measured by vibration sample magnometry, which revealed that the samples have soft ferromagnetic behavior. The microstructure and morphology were inspected by X-ray diffraction and scanning electron microscopy, respectively. Human cancer cells derived from the breast (MCF7) and oral cavity (C152) and normal cells derived from human umbilical vein endothelial cells (HUVECs) were treated with increasing concentrations of CoNi NPs, and their cytotoxic effect was measured via MTT and lactate dehydrogenase (LDH) leakage assays. We found that treatments by using 12.5 to 400 µg/mL of Co0.5Ni0.5, Co0.6Ni0.4, and Co0.4Ni0.6 NPs were associated with significant concentration-dependent toxicity toward such cell lines and profoundly enhanced LDH leakage following 48 h of exposure (P < 0.05 compared with untreated cells). Besides, a NP dose of 6.25 µg/mL did not affect the survival of HUVECs while leading to marked cell death in MCF7 and C152 cells. In vivo experiments in rats were done to investigate the biochemical and histopathological changes over three weeks, following intraperitoneal administration of Co0.5Ni0.5, Co0.6Ni0.4, and Co0.4Ni0.6 NPs (100 mg/kg). As compared with the controls, the exposure to NPs caused significant elevations in aspartate aminotransferase, alanine aminotransferase, blood urea nitrogen, serum creatinine, serum catalase activity, serum superoxide dismutase, and liver malondialdehyde levels. Also, rats treated with Co0.6Ni0.4 NPs showed more severe histopathological changes of the liver and kidney. Our findings represent an essential step toward developing theranostic nanoplatforms for selective cancer treatment.

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“…It was shown that cubic MNPs exhibit higher magnetization compared with spherical particles, possibly due to lower levels of disturbed spins on the surface (Noh et al, 2012). Also, cubic MNPs have a higher binding ability resulting from their higher contact area of planar interface (Danai et al, 2021). Additionally, stronger signals are produced via cubic MNPs.…”

Section: Synthesis Of Magnetic Nanoparticlesmentioning

confidence: 99%

The Potential Application of Magnetic Nanoparticles for Liver Fibrosis Theranostics

et al. 2021

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Liver fibrosis is a major cause of morbidity and mortality worldwide due to chronic liver damage and leading to cirrhosis, liver cancer, and liver failure. To date, there is no effective and specific therapy for patients with hepatic fibrosis. As a result of their various advantages such as biocompatibility, imaging contrast ability, improved tissue penetration, and superparamagnetic properties, magnetic nanoparticles have a great potential for diagnosis and therapy in various liver diseases including fibrosis. In this review, we focus on the molecular mechanisms and important factors for hepatic fibrosis and on potential magnetic nanoparticles-based therapeutics. New strategies for the diagnosis of liver fibrosis are also discussed, with a summary of the challenges and perspectives in the translational application of magnetic nanoparticles from bench to bedside.

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Synthesis and characterization of modified magnetic nanoparticles as theranostic agents: in vitro safety assessment in healthy cells

Cited by 13 publications

References 41 publications

Síntese de nanopartículas de óxido de ferro estabilizadas com citrato de sódio e TMAOH

Síntese de nanopartículas de óxido de ferro estabilizadas com citrato de sódio e TMAOH

CoNi alloy nanoparticles for cancer theranostics: synthesis, physical characterization, in vitro and in vivo studies

The Potential Application of Magnetic Nanoparticles for Liver Fibrosis Theranostics

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