Synthesis and Characterization of Diosgenin Encapsulated Poly-ε-Caprolactone-Pluronic Nanoparticles and Its Effect on Brain Cancer Cells

Abstract

Diosgenin encapsulated PCL-Pluronic nanoparticles (PCL-F68-D-NPs) were developed using the nanoprecipitation method to improve performance in brain cancer (glioblastoma) therapy. The nanoparticles were characterized by dynamic light scattering (DLS)/Zeta potential, Fourier-transform infrared (FTIR) spectra, X-ray diffraction (XRD), Field Emission Scanning Electron Microscopy (FESEM), and Transmission electron microscopy (TEM). The encapsulation efficiency, loading efficiency, and yield were calculated. The in vitro release rate was determined, and the kinetic model of diosgenin release was plotted and ascertained. The cytotoxicity was checked by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide)assay against U87-MG cells (glioblastoma cell lines). The obtained nanoparticles demonstrated good size distribution, stability, morphology, chemical, and mechanical properties. The nanoparticles also possessed high encapsulation efficiency, loading efficiency, and yield. The release rate of Diosgenin was shown in a sustained manner. The in vitro cytotoxicity of PCL-F68-D-NPs showed higher toxicity against U87-MG cells than free Diosgenin.

Green Synthesis of Silver Nanoparticles Using Diospyros malabarica Fruit Extract and Assessments of Their Antimicrobial, Anticancer and Catalytic Reduction of 4-Nitrophenol (4-NP)

“…On the other hand, the treated U87-MG cells with biosynthesized AgNPs depicted more apoptotic cells. The superimposed images divulged apprehension of the dead cells with a yellowish-orange and disfigured structure [ 3 , 94 , 95 ]. This result was in agreement with in vitro cytotoxicity assay (MTT analysis).…”

“…To study the functional group present on the synthesized AgNPs surface, FTIR analysis was performed by using an FTIR spectrophotometer (Perkin Elmer spectrum 100 FTIR, 710 Bridgeport, CT, USA). The FT-IR spectra were scanned with wave numbers ranging between 4000 and 400 cm −1 at a resolution of 4 cm −1 in the transmittance mode [ 3 ].…”

“…Nowadays, nanotechnology is a rapidly developing field and has a wide range of applications in biomedicine, drug delivery, bioimaging, bio-sensing devices, optoelectronics, catalysis and also in environmental protection due to the exemplary properties, such as biocompatibility, high productivity, rapid production and cost-effectiveness [ 1 , 2 , 3 , 4 , 5 ]. Among nanomaterials, metal nanoparticles, such as silver, copper, zinc, gold, titanium and magnesium, have been gaining immense magnitude for their applications and play a major role in this field [ 6 , 7 , 8 , 9 ].…”

In Vitro Interaction of Doxorubicin-Loaded Silk Sericin Nanocarriers with MCF-7 Breast Cancer Cells Leads to DNA Damage

“…Nanoparticles should display the desired characteristics such as size, morphology, and size distribution. Nanoprecipitation is a simple and fast method to produce nanoparticles from various types of polymers [ 33 , 34 , 35 , 36 , 37 , 38 ].…”

Development of Polymer-Based Nanoformulations for Glioblastoma Brain Cancer Therapy and Diagnosis: An Update

“…Various biodegradable polymeric drug delivery systems include nanogels or hydrogels, poly(ε-caprolactone) (PCL), poly (lactic-co-glycolic acid) (PLGA), chitosan [42,43], dendrimers, etc. [44]. Due to versatile tuneable properties, these nano- In low-grade gliomas, the structure and function of the BBTB resemble normal BBB, while in hi-grade glioma, BBB is significantly altered, disrupted.…”