In Vitro Interaction of Doxorubicin-Loaded Silk Sericin Nanocarriers with MCF-7 Breast Cancer Cells Leads to DNA Damage

Radu, I.; Zaharia, Cătălin; Hudiţă, Ariana; Tanasă, Eugenia; Ginghină, Octav; Marin, Minodora Maria; Gălățeanu, Bianca; Costache, Marieta

doi:10.3390/polym13132047

Cited by 21 publications

(13 citation statements)

References 55 publications

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“…Nanometric size provides numerous advantages such as better tissue penetration and significantly high cellular uptake [ 22 ]. Radu et al [ 23 ] mentioned that sericin polymeric nanoparticles showed potent properties, e.g., low toxicity due to their biodegradable nature. The present study was the first to report the synergistic activity and nanoformulation between sericin and Egyptian propolis extract.…”

Section: Resultsmentioning

confidence: 99%

Novel Amoxicillin-Loaded Sericin Biopolymeric Nanoparticles: Synthesis, Optimization, Antibacterial and Wound Healing Activities

Diab

Tayea

Elwakil

et al. 2022

IJMS

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Infected wounds are a major threat among diabetic patients. Technological advancements are currently increasing the number of new adjunctive therapies that may be potent agents for speeding recovery, lowering the amputation rate and limiting infection recurrences. A novel formula with promising antibacterial activity, namely sericin/propolis/Amoxicillin nanoparticles, was assessed as a potent treatment of infected wounds in normal and diabetic rats. Skin wound healing efficiency was assessed through wound healing scorings, bacterial load assessment and histological examinations. It was revealed that upon using sericin/propolis/Amoxicillin nanoparticles, complete wound healing was successfully achieved after 10 and 15 days postinjury for nondiabetic and diabetic rats, respectively. However, the bacterial load in the induced infected wounds was extremely low (0–10 CFU/mL) after 15 days post-treatment. The histological studies revealed that the dermis was more organized with new matrix deposition, and mature collagen fibers were observed among the treated animal groups. The present study is the first preclinical study which reported the importance of silk sericin in the form of nano-sericin/propolis loaded with Amoxicillin as an effective treatment against bacterial wound infections.

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Section: Resultsmentioning

confidence: 99%

Novel Amoxicillin-Loaded Sericin Biopolymeric Nanoparticles: Synthesis, Optimization, Antibacterial and Wound Healing Activities

Diab

Tayea

Elwakil

et al. 2022

IJMS

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“…To preserve a constant volume, after each sampling, 5 mL of fresh ultrapure water were added to every flask. The release efficiency ( RE ) was determined using Equation (1) [ 62 ]:

…”

Section: Methodsmentioning

confidence: 99%

Development of New Collagen/Clay Composite Biomaterials

Marin

Ianchiş

Alexa

et al. 2021

IJMS

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The fabrication of collagen-based biomaterials for skin regeneration offers various challenges for tissue engineers. The purpose of this study was to obtain a novel series of composite biomaterials based on collagen and several types of clays. In order to investigate the influence of clay type on drug release behavior, the obtained collagen-based composite materials were further loaded with gentamicin. Physiochemical and biological analyses were performed to analyze the obtained nanocomposite materials after nanoclay embedding. Infrared spectra confirmed the inclusion of clay in the collagen polymeric matrix without any denaturation of triple helical conformation. All the composite samples revealed a slight change in the 2-theta values pointing toward a homogenous distribution of clay layers inside the collagen matrix with the obtaining of mainly intercalated collagen-clay structures, according X-ray diffraction analyses. The porosity of collagen/clay composite biomaterials varied depending on clay nanoparticles sort. Thermo-mechanical analyses indicated enhanced thermal and mechanical features for collagen composites as compared with neat type II collagen matrix. Biodegradation findings were supported by swelling studies, which indicated a more crosslinked structure due additional H bonding brought on by nanoclays. The biology tests demonstrated the influence of clay type on cellular viability but also on the antimicrobial behavior of composite scaffolds. All nanocomposite samples presented a delayed gentamicin release when compared with the collagen-gentamicin sample. The obtained results highlighted the importance of clay type selection as this affects the performances of the collagen-based composites as promising biomaterials for future applications in the biomedical field.

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“…They also show greater toxicity of drugs towards cancer cells than drugs alone. 7,52,53 Some drugs, such as Naringenin (NAR) and Docetaxel (DXL), suffer from the drawbacks of poor water solubility, systemic toxicity, and slow dissolution rate. However, their anticancer efficacy is increased by using SF NPs for delivery.…”

Section: Silk Proteins In Drug-delivery Systemsmentioning

confidence: 99%

Research and therapeutic applications of silk proteins in cancer

et al. 2023

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Despite the availability of advanced treatments, cancer remains the second leading cause of death worldwide. This is due to the many challenges prevailing in the research field and cancer therapy. Resistance to therapy and side effects provide major hindrances to recovery from cancer. As a result, in addition to the aim of killing cancer cells, the focus should also be on reducing or preventing side effects of the treatment. To enhance the effectiveness of cancer treatment, many researchers are studying drug delivery systems based on silk proteins: fibroin and sericin. These proteins have high biocompatibility, biodegradability, and ease of modification. Consequently, many researchers have developed several formulations of silk proteins such as scaffolds, nanoparticles, and hydrogels by combining them with other materials or drugs. This review summarizes the use of silk proteins in various forms in cancer research and therapy. The use of silk proteins to study cancer cells, to deliver cancer drugs to a target site, in cancer thermal therapy, and as an anti-cancer agent is described here.

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In Vitro Interaction of Doxorubicin-Loaded Silk Sericin Nanocarriers with MCF-7 Breast Cancer Cells Leads to DNA Damage

Cited by 21 publications

References 55 publications

Novel Amoxicillin-Loaded Sericin Biopolymeric Nanoparticles: Synthesis, Optimization, Antibacterial and Wound Healing Activities

Novel Amoxicillin-Loaded Sericin Biopolymeric Nanoparticles: Synthesis, Optimization, Antibacterial and Wound Healing Activities

Development of New Collagen/Clay Composite Biomaterials

Research and therapeutic applications of silk proteins in cancer

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