Novel Amoxicillin-Loaded Sericin Biopolymeric Nanoparticles: Synthesis, Optimization, Antibacterial and Wound Healing Activities

Abstract

Infected wounds are a major threat among diabetic patients. Technological advancements are currently increasing the number of new adjunctive therapies that may be potent agents for speeding recovery, lowering the amputation rate and limiting infection recurrences. A novel formula with promising antibacterial activity, namely sericin/propolis/Amoxicillin nanoparticles, was assessed as a potent treatment of infected wounds in normal and diabetic rats. Skin wound healing efficiency was assessed through wound healing scorings, bacterial load assessment and histological examinations. It was revealed that upon using sericin/propolis/Amoxicillin nanoparticles, complete wound healing was successfully achieved after 10 and 15 days postinjury for nondiabetic and diabetic rats, respectively. However, the bacterial load in the induced infected wounds was extremely low (0–10 CFU/mL) after 15 days post-treatment. The histological studies revealed that the dermis was more organized with new matrix deposition, and mature collagen fibers were observed among the treated animal groups. The present study is the first preclinical study which reported the importance of silk sericin in the form of nano-sericin/propolis loaded with Amoxicillin as an effective treatment against bacterial wound infections.

In vitro and in vivo anti-colorectal cancer effect of the newly synthesized sericin/propolis/fluorouracil nanoplatform through modulation of PI3K/AKT/mTOR pathway

“…Sericin/propolis nanoparticles (nSE/PRO) were prepared according to Diab et al, 19 by adjusting different sericin to propolis ratio plus the stirring time of the reaction mixture. The suspensions attained were subjected to Zetasizer primitive characterization (Malvern Zetasizer Nano ZS, UK) to confirm the nanoparticles synthesis and stability 19 . Various Fluorouracil concentrations (10, 20 and 30 mg/ml) were added to the optimum trial then the combination index (CI) was determined.…”

Fungal Secondary Metabolites/Dicationic Pyridinium Iodide Combinations in Combat against Multi-Drug Resistant Microorganisms

“…Antibacterial activity was carried out using the disc-diffusion method; the discs were saturated with 25 µL of each fungal extract (20 mg/mL) and placed on the surface of inoculated Müeller–Hinton agar plates [ 30 ]. Further antibacterial activity evaluation was carried out by assessing the minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values [ 31 ].…”

Nano-Chitosan/Eucalyptus Oil/Cellulose Acetate Nanofibers: Manufacturing, Antibacterial and Wound Healing Activities

“…The antibacterial activity of the extracted oil was investigated using the disc diffusion method, and minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and MIC index assessments using the microdilution technique in accordance with Diab et al [ 22 ] and EUCAST [ 23 ]. The minimal inhibitory concentration was the lowest essential oil concentration at which no visible growth was detected, while the MBC was recorded when 99.9% of the bacterial population was killed at the lowest concentration of the extracted essential oil.…”

“…The antibacterial activity of the prepared nano-chitosan/oil (25 mg/mL) nanofiber was investigated using the disc diffusion method, MIC and MBC according to Diab et al [ 22 ]. Nano-chitosan/ Eucalyptus oil showed the highest antimicrobial activity and was selected for further analyses.…”