Synthesis and bioactivity of gelatin/multiwalled carbon nanotubes/hydroxyapatite nanofibrous scaffolds towards bone tissue engineering

Wang, Hualin; Chu, Chengjiang; Cai, Ruizhi; Jiang, Suwei; Zhai, Lipeng; Lu, Jianfeng; Li, Xingjiang; Jiang, Shaotong

doi:10.1039/c5ra07806g

Cited by 28 publications

(12 citation statements)

References 52 publications

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“…) [241], as well as extra components like silver nanoparticles [242], carbon nanotubes [243][244][245][246], graphene and graphene oxide [247,248], or magnetic nanoparticles (Fe 3 O 4 ) [93,94,249]. The presence of these dopants in HAp structure significantly contributes to the biostructure and biochemistry, similar to a natural bone [250].…”

Section: -mentioning

confidence: 99%

A brief review on hydroxyapatite production and use in biomedicine

et al. 2019

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Hydroxyapatite (HAp) is a bioceramic widely studied due to its chemical similarity with the mineral component of bones. Besides, it is biocompatible, bioactive and thermodynamically stable in the body fluid what poses it as an attractive material for a wide range of applications in the biomedical field. Several efforts have been focused on the synthesis of particles of this material aiming to the precise control of size and morphology, porosity and surface area. HAp is widely used as an implant for bone tissue regeneration, as a coating for metallic implants and in a drug-controlled release. In this sense, the objective of this review is to gather information related to HAp, providing readers with information about synthesis methods, material characteristics and their applications.

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Section: -mentioning

confidence: 99%

A brief review on hydroxyapatite production and use in biomedicine

et al. 2019

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“…These are the principal diffraction peaks of HA, according to the JCPDS card no. 09-0432 [37]. Apart from the HA-free scaffold, all fabricated scaffolds containing HA exhibited distinct characteristic HA peaks.…”

Section: Chemical and Structural Properties Of Ha/gelatin Composite Scaffoldsmentioning

confidence: 99%

“…The FTIR spectra of all fabricated scaffolds display a series of amide and carboxyl bands corresponding to the chemical structure of gelatin (Figure 3b). The peaks at 1644, 1536, and 1239 cm −1 were attributed to the C=O stretching (amide I), N-H bending (amide II), and N-H stretching (amide III) vibrations, respectively [37], while the peak at 1450 cm −1 was associated with carboxyl groups [38]. For an effective analysis of the HA vibrational properties, the FTIR spectrum of pure HA powder was also measured and compared with those of the scaffolds.…”

Section: Chemical and Structural Properties Of Ha/gelatin Composite Scaffoldsmentioning

confidence: 99%

Additive Fabrication and Characterization of Biomimetic Composite Bone Scaffolds with High Hydroxyapatite Content

Lee

Nam

2021

Gels

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With the increased incidence of bone defects following trauma or diseases in recent years, three-dimensional porous scaffolds fabricated using bioprinting technologies have been widely explored as effective alternatives to conventional bone grafts, which provide cell-friendly microenvironments promoting bone repair and regeneration. However, the limited use of biomaterials poses a significant challenge to the robust and accurate fabrication of bioprinted bone scaffolds that enable effective regeneration of the target tissues. Although bioceramic/polymer composites can provide tunable biomimetic conditions, their effects on the bioprinting process are unclear. Thus, in this study, we fabricated hydroxyapatite (HA)/gelatin composite scaffolds containing large weight fractions of HA using extrusion-based bioprinting, with the aim to provide an adequate biomimetic environment for bone tissue regeneration with compositional and mechanical similarity to the natural bone matrix. The overall features of the bioprinted HA/gelatin composite scaffolds, including rheological, morphological, physicochemical, mechanical, and biological properties, were quantitatively assessed to determine the optimal conditions for both fabrication and therapeutic efficiency. The present results show that the bioprinted bioceramic/hydrogel scaffolds possess excellent shape fidelity; mechanical strength comparable to that of native bone; and enhanced bioactivity in terms of cell proliferation, attachment, and osteogenic differentiation. This study provides a suitable alternative direction for the fabrication of bioceramic/hydrogel-based scaffolds for bone repair based on bioprinting.

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“…Gelatin presents a high biocompatibility and biodegradability, with minor antigenicity respect to collagen . It contains abundant arginine–glycine–aspartic (RGD) sequences useful to favor cell adhesion and several functional groups allowing chemical modifications (i.e., coupling with cross‐linkers and targeting ligands) . All these properties make gelatin suitable as ideal biomaterial for wide applications in TE .…”

Section: Introductionmentioning

confidence: 99%

“…8 It contains abundant arginineglycine-aspartic (RGD) sequences useful to favor cell adhesion 9 and several functional groups allowing chemical modifications (i.e., coupling with cross-linkers and targeting ligands). 10,11 All these properties make gelatin suitable as ideal biomaterial for wide applications in TE. 9,12,13 Gelatin scaffolds are structures lacking of nanofibrous architectures on the submicrometer scale (10-100 nm in diameter) and are mechanically weaker than native heart tissues.…”

Section: Introductionmentioning

confidence: 99%

Cardiac tissue regeneration: A preliminary study on carbon‐based nanotubes gelatin scaffold

et al. 2017

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The aim of this study was set-up and test of gelatin and carbon nanotubes scaffolds. Gelatin-based (5%) genipin cross-linked (0.2%) scaffolds embedding single-walled carbon nanotubes (SWCNTs, 0.3, 0.5, 0.7, 0.9, and 1.3% w/w) were prepared and mechanically/electrically characterized. For biological evaluation, H9c2 cell line was cultured for 10 days. Cytotoxicity, cell growth and differentiation, immunohistochemistry, and real-time PCR analysis were performed. Myoblast and cardiac differentiation were obtained by serum reduction to 1% (C ) and stimulation with 50 nM all trans-retinoic acid (C ), respectively. Immunohistochemistry showed elongated myotubes in C while round and multinucleated cells in C with also a significantly increased expression of natriuretic peptides (NP) and ET-1 receptors in parallel with a decreased ET-1. On scaffolds, cell viability was similar for Gel-SWCNT ; NP and ET systems expression decreased in both concentrations with respect to control and CX-43, mainly due to a lacking of complete differentiation in cardiac phenotype during that time. Although further analyses on novel biomaterials are necessary, these results represent a useful starting point to develop new biomaterial-based scaffolds. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2750-2762, 2018.

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Synthesis and bioactivity of gelatin/multiwalled carbon nanotubes/hydroxyapatite nanofibrous scaffolds towards bone tissue engineering

Abstract: The in vitro bioactivity of scaffolds, and the adhesion, mineralization, viability and proliferation of hFOBs on gelatin/MWNTs/HA nanofibrous scaffolds.

Cited by 28 publications

References 52 publications

A brief review on hydroxyapatite production and use in biomedicine

A brief review on hydroxyapatite production and use in biomedicine

Additive Fabrication and Characterization of Biomimetic Composite Bone Scaffolds with High Hydroxyapatite Content

Cardiac tissue regeneration: A preliminary study on carbon‐based nanotubes gelatin scaffold

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