Synonomy of Peptococcus glycinophilus (Cardon and Barker 1946) Douglas 1957 with Peptostreptococcus micros (Prevot 1933) Smith 1957 and Electrophoretic Differentiation of Peptostreptococcus micros from Peptococcus magnus (Prevot 1933) Holdeman and Moore 1972

Cato, Elizabeth P.; Johnson, John L.; Hash, D. E.; Holdeman, Lillian V.

doi:10.1099/00207713-33-2-207

Cited by 21 publications

(7 citation statements)

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“…To date, little work has been done on the genus Peptostreptococcus. Cato et al (2) suggested that two strains identified as P. magnus, type strain ATCC 15794 and ATCC 14955, originally deposited as the type strain of "Micrococcus variabilis," are distinct, although they refrained from proposing a revival of the M. variabilis name.…”

Section: Discussionmentioning

confidence: 99%

Enzymatically active Peptostreptococcus magnus: association with site of infection

et al. 1992

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Fifty-four strains of Peptostreptococcus magnus (11 were recovered from abdominal infections, 18 were from nonpuerperal breast abscesses, and 21 were from diabetic foot infections; the type strain and three other strains were from the American Type Culture Collection, Rockville, Md.) and the type strain of Peptostreptococcus micros were tested for their ability to produce various enzymes, including catalase, hippurate hydrolase, serine dehydratase, threonine dehydratase, collagenase, gelatinase, alkaline phosphatase, and esterase C4. The data were analyzed by cluster analysis. The results showed that all but one strain could be assigned to either of two distinct, valid clusters. The first cluster of 11 strains was composed of strains that were relatively inactive, having produced one or two of the eight strain-dependent enzymes. The second was a large cluster of strains (n = 43) that were considerably more active, all having produced at least three enzymes; the vast majority of strains (89%o) produced four or more enzymes. The unclustered strain produced one enzyme that was different from that produced by the strains in the first cluster. The x2 test of homogeneity applied to the clustering solution indicated that greater enzyme activity was significantly associated with the site of infection (P < 0.001). The more enzymatically active P. magnus strains were recovered significantly more often from nonpuerperal breast abscesses and diabetic foot infections than they were from abdominal infections. These results may provide insight into the nature of certain polymicrobial soft tissue infections and suggest that (i) P. magnus may participate more in nonpuerperal breast and diabetic foot infections than in abdominal infections and that (ii) peptostreptococcal production of proteolytic enzymes may have an important adjunctive effect on the pathogenesis of certain soft tissue infections.

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Section: Discussionmentioning

confidence: 99%

Enzymatically active Peptostreptococcus magnus: association with site of infection

et al. 1992

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“…Within the Peptococcaceae, P. magnus, P. micros, and P. anaerobius are generally accepted as being phylogenetically valid species. Peptococcus glycinophilus was shown to be a synonym of P. micros by Cato et al (58), who compared the soluble cell protein patterns of the type strains by polyacrylamide gel electrophoresis (PAGE). The classification of Peptostreptococcus productus is under active review.…”

Section: Application Of Nucleic Acid Techniquesmentioning

confidence: 99%

“…Ezaki et al (87) reported that 22 of 23 strains of P. magnus examined formed catalase or liquified gelatin, but the type strain of P. micros was negative for both tests; they suggested that these tests could be "helpful." Cato et al (58) recommended a comparison of soluble-cell protein patterns by PAGE, but this technique is unlikely to be of use outside a research laboratory. Cato et al (58) also compared 10 reference strains of P. magnus by using the API ZYM kit (Analytab Products) and reported that none formed ALP; Holdeman Moore et al (136) recommended this test in Bergey's Manual of Systematic Bacteriology in 1986.…”

Section: P Magnusmentioning

confidence: 99%

Gram-Positive Anaerobic Cocci

1998

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SUMMARY Gram-positive anaerobic cocci (GPAC) are a heterogeneous group of organisms defined by their morphological appearance and their inability to grow in the presence of oxygen; most clinical isolates are identified to species in the genus Peptostreptococcus. GPAC are part of the normal flora of all mucocutaneous surfaces and are often isolated from infections such as deep organ abscesses, obstetric and gynecological sepsis, and intraoral infections. They have been little studied for several reasons, which include an inadequate classification, difficulties with laboratory identification, and the mixed nature of the infections from which they are usually isolated. Nucleic acid studies indicate that the classification is in need of radical revision at the genus level. Several species of Peptostreptococcus have recently been described, but others still await formal recognition. Identification has been based on carbohydrate fermentation tests, but most GPAC are asaccharolytic and use the products of protein degradation for their metabolism; the introduction of commercially available preformed enzyme kits affords a physiologically more appropriate method of identification, which is simple and relatively rapid and can be used in routine diagnostic laboratories. Recent reports have documented the isolation in pure culture of several species, notably Peptostreptococcus magnus, from serious infections. Studies of P. magnus have elucidated several virulence factors which correlate with the site of infection, and reveal some similarities to Staphylococcus aureus. P. micros is a strongly proteolytic species; it is increasingly recognized as an important pathogen in intraoral infections, particularly periodontitis, and mixed anaerobic deep-organ abscesses. Comparison of antibiotic susceptibility patterns reveals major differences between species. Penicillins are the antibiotics of choice, although some strains of P. anaerobius show broad-spectrum β-lactam resistance.

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“…Harpold & Wasilauskas (17) have also reported the application of high-performance liquid chromatography in analyzing the changes of a chemically defined medium after the bacterial utilization of added amino acids. The patterns of cellular proteins have been used in the differentiation of P. micros from other anaerobic gram-positive cocci (6,33). In addition, Collins et al (7) have demonstrated the employment of the indirect fluorescent antibody test in the identification of P. micros from oral lesions.…”

Section: Discussionmentioning

confidence: 99%

Development and evaluation of a selective and differential medium for the primary isolation ofPeptostreptococcus micros

Turng

Guthmiller

Minah

et al. 1996

Oral Microbiology and Immunology

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Peptostreptococcus micros, an anaerobic gram-positive coccus, has been associated with periodontal and endodontic lesions, including those refractory to treatment, as well as many human polymicrobial infections in other body locations. A selective and differential medium for the primary isolation of P. micros was developed and evaluated. Columbia CNA agar, a selective medium for gram-positive cocci, was supplemented with glutathione and lead acetate (P. micros medium: PMM). P. micros has a characteristic of rapidly utilizing the reduced form of glutathione to form hydrogen sulfide, which reacts with lead acetate producing a black precipitate in the medium. When grown on PMM, P. micros can be easily identified by its typical colonial morphology and the presence of a black precipitate directly under the colony. PMM was compared for the growth of P. micros with phenylethyl alcohol agar (PEA) and Columbia base medium (CBM) with 80 strains of P. micros and 30 strains of other gram-positive cocci. All P. micros isolates tested grew and showed the typical morphology of P. micros on PMM. Using colony counts on CBM as controls, there was an average 81.8% recovery in the number of P. micros colonies on PMM, in contrast to an average 6.1% recovery on PEA. Subgingival plaque and tongue samples from 12 adult periodontitis and 6 early-onset periodontitis patients were cultured onto PMM for the isolation of P. micros. P. micros was isolated on PMM and identified biochemically and enzymatically from both adult periodontitis and early-onset periodontitis patients with higher percentages isolated from the diseased periodontal pockets of adult periodontitis patients; furthermore, this is the first isolation of P. micros from tongue samples taken from periodontally diseased patients. This medium in cultural studies will further our understanding and assist future investigations of P. micros involved in disease processes.

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Synonomy of Peptococcus glycinophilus (Cardon and Barker 1946) Douglas 1957 with Peptostreptococcus micros (Prevot 1933) Smith 1957 and Electrophoretic Differentiation of Peptostreptococcus micros from Peptococcus magnus (Prevot 1933) Holdeman and Moore 1972

Cited by 21 publications

References 1 publication

Enzymatically active Peptostreptococcus magnus: association with site of infection

Enzymatically active Peptostreptococcus magnus: association with site of infection

Gram-Positive Anaerobic Cocci

Development and evaluation of a selective and differential medium for the primary isolation ofPeptostreptococcus micros

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