1996
DOI: 10.1111/j.1399-302x.1996.tb00194.x
|View full text |Cite
|
Sign up to set email alerts
|

Development and evaluation of a selective and differential medium for the primary isolation ofPeptostreptococcus micros

Abstract: Peptostreptococcus micros, an anaerobic gram-positive coccus, has been associated with periodontal and endodontic lesions, including those refractory to treatment, as well as many human polymicrobial infections in other body locations. A selective and differential medium for the primary isolation of P. micros was developed and evaluated. Columbia CNA agar, a selective medium for gram-positive cocci, was supplemented with glutathione and lead acetate (P. micros medium: PMM). P. micros has a characteristic of ra… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
18
0
1

Year Published

2001
2001
2021
2021

Publication Types

Select...
7
1

Relationship

0
8

Authors

Journals

citations
Cited by 19 publications
(19 citation statements)
references
References 32 publications
0
18
0
1
Order By: Relevance
“…P. micros is commonly identi®ed on the basis of colony morphology, oxygen tolerance and Gram's staining characteristics, followed by analysis of its enzymic activities (API ZYM, Rapid ID 32A) and its capacity to hydrolyse amino acid and phosphate substrates (RapID ANA II) [5,18]. A selective and differential medium has been described that facilitates the primary isolation of P. micros from clinical samples which is based upon a Columbia CNA agar, supplemented with glutathione and lead acetate [15], that is selective for gram-positive cocci. When grown on this medium, P. micros utilises the reduced form of glutathione to form H 2 S, which reacts with lead acetate to produce a black precipitate that is clearly visible below P. micros colonies.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…P. micros is commonly identi®ed on the basis of colony morphology, oxygen tolerance and Gram's staining characteristics, followed by analysis of its enzymic activities (API ZYM, Rapid ID 32A) and its capacity to hydrolyse amino acid and phosphate substrates (RapID ANA II) [5,18]. A selective and differential medium has been described that facilitates the primary isolation of P. micros from clinical samples which is based upon a Columbia CNA agar, supplemented with glutathione and lead acetate [15], that is selective for gram-positive cocci. When grown on this medium, P. micros utilises the reduced form of glutathione to form H 2 S, which reacts with lead acetate to produce a black precipitate that is clearly visible below P. micros colonies.…”
Section: Discussionmentioning
confidence: 99%
“…A selective and differential medium for the primary isolation of P. micros has been described [15]. Monoclonal antibodies (MAbs) recognising speci®c surface antigens of P. micros have been generated and successfully applied to its detection in clinical samples in an indirect immuno¯uorescence assay [16].…”
Section: Introductionmentioning
confidence: 99%
“…36 Bacteria known to produce volatile sulphur-containing compounds include Aggregatibacter actinomycetemcomitans (formerly Actinobacillus actinomycetemcomitans), Actinomyces species, Atopobium parvulum, Campylobacter rectus, Desulfovibrio species, Eikenella corrodens, Eubacterium sulci, Fusobacterium species, Peptostreptococcus micros, Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella species, Solobacterium moorei, Tannerella forsythia (formerly Bacteriodes forsythus or Tannerella forsythensis), Treponema denticola, Veillonella species, Vibrio species, a phylotype of Dialister, a phylotype of the uncultivated phylum, and a phylotype of Streptococcus, and as yet unidentified sulphur-reducing bacteria. 22,[33][34][35][37][38][39][40][41][42][43][44][45][46] The species diversity found in halitosis samples suggests that halitosis may be the result of complex interactions between several bacterial species. Also, the role of uncultivable bacteria may be important in contributing to this complex process.…”
Section: Oral Malodourmentioning
confidence: 97%
“…The following media were used: Schaedlers medium (STA) to enumerate total anaerobic bacteria; Schaedlers Nalidixic/Vancomycin medium (SNV) to enumerate total Gram-negative anaerobes; and OOPS medium supplemented with 5% sheep's red blood cells (El-Halabi et al 1999) to enumerate VSC-producing organisms (Turng et al 1996). Dilutions of 10 À 2 and 10 À 4 were plated in duplicate on selective media using a spiral plater.…”
Section: Microbiological Proceduresmentioning
confidence: 99%