Synergistic maximization of the carbohydrate output and lignin processability by combinatorial pretreatment

Abstract

Combinatorial pretreatments with a low holding temperature were developed in an effort to synergistically improve the carbohydrate output and lignin processability from corn stover.

Editorial: Advancements in Biomass Recalcitrance: The Use of Lignin for the Production of Fuels and Chemicals

“…these efforts, the conversion of lignin to chemicals and fungible fuels for ground and aviation transportation is being aggressively developed using thermal and/or catalytic processes (Ben and Ragauskas, 2011;Bi et al, 2015). As an alternative to these chemical technologies, the power of biology is also being investigated to convert lignin to fatty acids and esters using Rhodococcus (Le et al, 2017), and Pseudomonas putida for PHAs (Liu et al, 2017), to name just a few promising pathways.…”

Emerging Strategies for Modifying Lignin Chemistry to Enhance Biological Lignin Valorization

“…Meanwhile, lignin output in the liquid stream from this combinatorial pretreatment increased by 33.4 %. [13] These strategies not only significantly improved the lignin output to produce a comparable level of coproducts but also synergistically enhanced the release of fermentable sugars from carbohydrates in a biorefinery.…”

“…[4a,12a,13] Nuclear magnetic resonance (NMR) characterization of the fractionated lignin demonstrated that guaiacyl (G) and p-hydroxyphenyl (H) units were increased whereas β-β and β-O-4 linkages were disrupted after the optimal combinatorial pretreatment. [13] Meanwhile, 31 P NMR results indicated that combinatorial pretreatments led to fewer aliphatic OH but more phenolic OH and carboxylic acid groups. Most importantly, aromatic monomers were generated during the optimal combinatorial pretreatment to facilitate lignin bioconversion.…”

Bioconversion of lignin and its derivatives into polyhydroxyalkanoates: Challenges and opportunities

“…The same research group has also reported another recombinant strain P. putida KT2440. Here, enhanced lignin degradation was achieved by combinatorial treatments . Under shake flask conditions, the strain was able to grow up to 2.53 g/L in biomass containing 14.1% PHA, whereas under fed‐batch cultivation, the lignin‐rich substrate yielded a maximum PHA of 1.0 g/L (Table ).…”

“…To circumvent these issues, researchers have identified ATP‐binding cassettes capable of transporting quite a few benzoate derivatives including vanillic acid and 4‐HBA . Lignin utilization by combinatorial pretreatments is another approach where the enzymatic degradation is exploited by the assistance of either fungi or bacteria . Fungi‐associated lignin pretreatment has several advantages such as low‐energy consumption, minimal waste, and inhibitor generation with simple operations.…”