Bioconversion of lignin and its derivatives into polyhydroxyalkanoates: Challenges and opportunities

Kumar, Prasun; Maharjan, Anoth; Park, Jun-Gyu; Kim, Beom Soo

doi:10.1002/bab.1720

Cited by 29 publications

(14 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7A) (Pohlmann et al ., 2006; Brigham et al ., 2012). Although the H16 strain has been shown to accumulate PHB granules when grown on a wide range of substrates, including certain aromatic compounds such as 4‐hydroxybenzoate, 3‐hydroxybenzoate, protocatechuate, gentisate, (Tomizawa et al ., 2014; Kumar et al ., 2019), the strain has never been tested for bioconversion of phthalates. Since we have shown above that the pht pathway is functional in strain H16, we tested whether C. necator H16 (pIZPHTRAP) cells grown aerobically on PA were able to accumulate PHB.…”

Section: Resultsmentioning

confidence: 99%

Expanding the current knowledge and biotechnological applications of the oxygen‐independent ortho‐phthalate degradation pathway

Sanz

Garcı́a

Dı́az

2020

Environmental Microbiology

View full text Add to dashboard Cite

Summary ortho‐Phthalate derives from industrially produced phthalate esters, which are massively used as plasticizers and constitute major emerging environmental pollutants. The pht pathway for the anaerobic bacterial biodegradation of o‐phthalate involves its activation to phthaloyl‐CoA followed by decarboxylation to benzoyl‐CoA. Here, we have explored further the pht peripheral pathway in denitrifying bacteria and shown that it requires also an active transport system for o‐phthalate uptake that belongs to the poorly characterized class of TAXI‐TRAP transporters. The construction of a fully functional pht cassette combining both catabolic and transport genes allowed to expand the o‐phthalate degradation ecological trait to heterologous hosts. Unexpectedly, the pht cassette also allowed the aerobic conversion of o‐phthalate to benzoyl‐CoA when coupled to a functional box central pathway. Hence, the pht pathway may constitute an evolutionary acquisition for o‐phthalate degradation by bacteria that thrive either in anoxic environments or in environments that face oxygen limitations and that rely on benzoyl‐CoA, rather than on catecholic central intermediates, for the aerobic catabolism of aromatic compounds. Finally, the recombinant pht cassette was used both to screen for functional aerobic box pathways in bacteria and to engineer recombinant biocatalysts for o‐phthalate bioconversion into sustainable bioplastics, e.g., polyhydroxybutyrate, in plastic recycling industrial processes.

show abstract

Section: Resultsmentioning

confidence: 99%

Expanding the current knowledge and biotechnological applications of the oxygen‐independent ortho‐phthalate degradation pathway

Sanz

Garcı́a

Dı́az

2020

Environmental Microbiology

View full text Add to dashboard Cite

show abstract

“…Acinetobacter, Azospirillum, Pseudomonas, Brevundimonas, Devosia, Achromobacter, and Chryseobacterium play major roles in this process. Acinetobacter is found in cellulose-containing agricultural waste as the only carbon source, and e ciently secretes extracellular cellulase and hemicellulose enzymes 28,29 ; Azospirillum has been shown to produce hydrogen peroxide enzymes, oxidase, methyl cellulase, and produce acetic acid, butyric acid, and lactic acid, and to participate in straw degradation metabolism 30,31 ; Dye-decolonizing peroxidases (DYPs) secreted by Pseudomonas have the ability to degrade lignin and lignin model compounds 32,33 and have high laccase and lignin peroxidase activities 34 . They are believed to be important functional bacteria for degradation of straw lignin.…”

Section: Discussionmentioning

confidence: 99%

Microbial Community Succession is Associated With Corn Straw Degradation in Microbial Consortium M44 During Subculture

Zhang

Qing

Gao

et al. 2021

Preprint

View full text Add to dashboard Cite

To systematically analyze the succession of functional microbiota that plays an important role during culture of microbial consortia M44 and its relationship with straw degradation characteristics, we determined the straw degradation ratio and activities of cellulose, hemicellulose, lignin enzyme, and VFA content of M44 in different culture periods. We also used 16S rRNA gene sequencing to analyze the change in microbial community structure in M44 and explore the differences in microbial composition in the original sample. The results showed that at 15 ℃ for 21 days, the straw degradation rate, endoglucanase activity, and filter paper enzyme activity of M44 generally decreased with increasing culture age, reaching their highest values at F1. The activities of xylanase, laccase, and lignin peroxidase, as well as VFA content, were the highest at F5, showing a single-peak curve change with first an increase and then decrease. At the phylum level, Proteobacteria, Bacteroidetes, and Firmicutes were dominant in the original samples and in different culture stages. At the genus level, Devosia and Bacillus were dominant in the original sample. During subculture, the dominant bacteria in the first generation (F1) were Pseudomonas, Flavobacterium, Brevundimonas, Achromobacter, Chryseobacterium, and Devosia. The dominant genera in the last generation (F11) were Trichococcus, Acinetobacter, Dyssgonomonas, and Rhizobium. In conclusion, we identified changes in microbial community structure occurring in M44 during subculture, as well as similarities and differences in microbial communities from the original sample.

show abstract

“…There are various bacteria capable of producing PHA from lignin-based aromatics [16, 50, 108–111]. In addition to aromatic monomers, some actual lignin streams can also be utilized for PHA biosynthesis [112, 113] (Table 3). Just like lipid production from lignin-based streams, the PHA production from lignin streams by bacterial cultures is also generally concurrent with the reduction of the liquor color and COD [108, 114].…”

Section: Application Of Multiple Metabolic Pathways For Lignin Valorimentioning

confidence: 99%

Recent advances in lignin valorization with bacterial cultures: microorganisms, metabolic pathways, and bio-products

Lei²,

Zhai

et al. 2019

Biotechnol Biofuels

199

119

View full text Add to dashboard Cite

Lignin is the most abundant aromatic substrate on Earth and its valorization technologies are still under developed. Depolymerization and fragmentation are the predominant preparatory strategies for valorization of lignin to chemicals and fuels. However, due to the structural heterogeneity of lignin, depolymerization and fragmentation typically result in diverse product species, which require extensive separation and purification procedures to obtain target products. For lignin valorization, bacterial-based systems have attracted increasing attention because of their diverse metabolisms, which can be used to funnel multiple lignin-based compounds into specific target products. Here, recent advances in lignin valorization using bacteria are critically reviewed, including lignin-degrading bacteria that are able to degrade lignin and use lignin-associated aromatics, various associated metabolic pathways, and application of bacterial cultures for lignin valorization. This review will provide insight into the recent breakthroughs and future trends of lignin valorization based on bacterial systems.

show abstract

Bioconversion of lignin and its derivatives into polyhydroxyalkanoates: Challenges and opportunities

Cited by 29 publications

References 65 publications

Expanding the current knowledge and biotechnological applications of the oxygen‐independent ortho‐phthalate degradation pathway

Expanding the current knowledge and biotechnological applications of the oxygen‐independent ortho‐phthalate degradation pathway

Microbial Community Succession is Associated With Corn Straw Degradation in Microbial Consortium M44 During Subculture

Recent advances in lignin valorization with bacterial cultures: microorganisms, metabolic pathways, and bio-products

Contact Info

Product

Resources

About