Synergistic interaction caused to human gingival fibroblasts from dental monomers

Durner, Jürgen; Wellner, P.; Hickel, Reinhard; Reichl, Franz‐Xaver

doi:10.1016/j.dental.2012.04.031

Cited by 31 publications

(26 citation statements)

References 27 publications

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“…The cytotoxic effects on human gingival fibroblasts caused by biomaterials commonly used in restorative dentistry, have been the subject of considerable attention [25]. The antimicrobial efficacy and biocompatibility of the thermosets used in this study were also investigated previously in applications for orthopedic devices [8], [9], [26].…”

Section: Discussionmentioning

confidence: 99%

Biological Responses of Human Gingival Fibroblasts (HGFs) in an Innovative Co-Culture Model with Streptococcus mitis to Thermosets Coated with a Silver Polysaccharide Antimicrobial System

et al. 2014

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This study sought to evaluate the in vitro biological response of human gingival fibroblasts (HGFs) co-coltured with Streptococcus mitis to bisphenol A glycidylmethacrylate/triethylene glycol dimethacrylate (BisGMA/TEGDMA) thermosets coated with Chitlac-nAg, a nanocomposite system with antimicrobial properties. To avoid bacterial adhesion to dental devices and to reduce cytotoxicity against eukaryotic cells, we coated BisGMA/TEGDMA methacrylic thermosets with a new material, Chitlac-nAg, formed by stabilizing silver nanoparticles, which have well-known antimicrobial properties, with a polyelectrolyte solution containing Chitlac. Cytotoxicity, cell morphology, cell migration and inflammatory interleukine-6 (IL-6) and prostaglandin E2 (PGE2) secretion were evaluated. Our results showed that the cytotoxicity exerted on HGFs by our nanocomposite material was absent in our co-culture model, where fibroblasts are able to adhere and migrate. After 24 h thermosets coated with Chitlac as well as those coated with Chitlac-nAg exerted a minimal cytotoxic effect on HGFs, while after 48 h LDH release rises up 20%. Moreover the presence of S. mitis reduced this release in a greater amount with Chitlac-nAg coated thermosets. The secretion of IL-6 was significant in both Chitlac and Chitlac-nAg coated thermosets, but PGE2 production was minimal, suggesting that the IL-6 production was not related to an inflammatory response. Co-culture and the addiction of saliva did not influence IL-6 and PGE2 secretion. Data obtained in the present work suggest that Chitlac n-Ag coated thermosets could significantly improve the success rates of restorative dentistry, since they limit bacterial adhesion and are not toxic to HGFs.

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Section: Discussionmentioning

confidence: 99%

Biological Responses of Human Gingival Fibroblasts (HGFs) in an Innovative Co-Culture Model with Streptococcus mitis to Thermosets Coated with a Silver Polysaccharide Antimicrobial System

et al. 2014

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“…For example it could be shown in two in vitro studies, that at 40 °C a 50% shorter curing time leads to the same degree of conversion [15,16]. On the other hand, the same curing time results in less residual monomer, minimizing the risk of hypoallergenic reactions [17,18,19,20]. Another study reported less leakage in class II restorations by using a preheated RC [21].…”

Section: Introductionmentioning

confidence: 99%

Flexural Strength of Preheated Resin Composites and Bonding Properties to Glass-Ceramic and Dentin

2016

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To test the impact of preheating (25, 37, 54, or 68 °C) of TetricEvoCeram (TEC), FiltekSupremeXT (FSXT), and Venus (V) on flexural strength (FS), shear bond strength (SBS) and interfacial tension (IFT). FS was tested with TEC and FSXT. For SBS, glass-ceramic and human dentin substrate were fabricated and luted with the preheated resin composite (RC). SBSs of 1500 thermal cycled specimens were measured. For IFT, glass slides covered with the non-polymerized RC were prepared and contact angles were measured. Data were analyzed using 2/1-way ANOVA with Scheffé-test, and t-test (p < 0.05). Preheated TEC (37–68 °C) showed higher FS compared to the control-group (25 °C) (p < 0.001). FSXT presented higher FS than TEC (p < 0.001). For SBS to dentin higher values for FSXT than TEC were found. The preheating temperature showed no impact on SBS to dentin. SBS to glass-ceramic revealed a positive influence of temperature for TEC 25–68 °C (p = 0.015). TEC showed higher values than V and FSXT (p < 0.001). IFT values increased with the preheating temperature. A significant difference could be observed in every RC group between 25 and 68 °C (p < 0.001).

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“…WisniewskaJarosinska et al 8) reported that a combination of both UDMA at 1 mM and TEGDMA at 5 mM did not produce a significant increase in cytotoxic effects on Chinese hamster ovary cells when compared to each compound applied individually. Durner et al 32) found that mixtures of TEGDMA at 1.60 mM and UDMA at 0.04 mM had a synergistic interaction on human gingival fibroblasts. In contrast, Ratanasathien et al 5) evaluated the cytotoxic effects of TEGDMA and UDMA in Balb/c 3T3 mouse fibroblasts and determined an antagonistic interaction between them.…”

Section: Toxicity Analysismentioning

confidence: 99%

Evaluation of residual monomer release and toxicity of self-adhesive resin cements

Kurt

Altıntaş

Kızıltaş

et al. 2018

Dental Materials Journal

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The aim of this study was to evaluate the amount of leached residual monomers from self-adhesive resin cements and evaluate their toxicity in-vitro. A total of 60 disk-shaped specimens (5 mm in diameter and 0.5 mm in thickness) were prepared from each cement (RelyX U200, SpeedCEM, G-Cem) (n=20). Specimens were immersed in artificial saliva and the amount of released monomers [urethane dimethacrylate (UDMA) and triethyleneglycol dimethacrylate (TEGDMA)] was identified. Then, the cytotoxicity and genotoxicity effect on cells were evaluated using the defined amounts of released monomers from cements. The highest monomer release was detected in G-Cem (p<0.05). The highest cytotoxicity value was identified from SpeedCEM (p<0.01) and the highest genotoxicity values were calculated from RelyX U200 (p<0.05). Released UDMA and TEGDMA from self-adhesive resin cements induced cytotoxicity and genotoxicity effect on cells.

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Synergistic interaction caused to human gingival fibroblasts from dental monomers

Cited by 31 publications

References 27 publications

Biological Responses of Human Gingival Fibroblasts (HGFs) in an Innovative Co-Culture Model with Streptococcus mitis to Thermosets Coated with a Silver Polysaccharide Antimicrobial System

Biological Responses of Human Gingival Fibroblasts (HGFs) in an Innovative Co-Culture Model with Streptococcus mitis to Thermosets Coated with a Silver Polysaccharide Antimicrobial System

Flexural Strength of Preheated Resin Composites and Bonding Properties to Glass-Ceramic and Dentin

Evaluation of residual monomer release and toxicity of self-adhesive resin cements

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