Synergistic effects of mechanical stimulation and crimped topography to stimulate natural collagen development for tendon engineering

Chen, Zhenying; Zhou, Boya; Wang, Xiansong; Zhou, Guangdong; Zhang, Wenjie; Yi, Baolian; Wang, Wenbo; Liu, Wei

doi:10.1016/j.actbio.2022.04.026

Cited by 18 publications

(21 citation statements)

References 48 publications

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“…Second, different doses of LMK235 treatment may lead to different patterns of DEGs. This phenomenon is also revealed in other studies and showed that LMK235 is a multitarget and multi-functional compound dependent on its dosage and cell types [ 58 , 59 , 60 , 61 , 62 , 63 ]. Therefore, we investigated the overlapping DEGs of 0.5 μM and 2 μM that inhibited cell viability.…”

Section: Discussionsupporting

confidence: 69%

A Selective Histone Deacetylase Inhibitor Induces Autophagy and Cell Death via SCNN1A Downregulation in Glioblastoma Cells

Chang

Tsai

et al. 2022

Cancers

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Glioblastoma multiforme (GBM) is a grade IV, highly malignant brain tumor. Because of the heterogeneity of GBM, a multitarget drug is a rational strategy for GBM treatment. Histone deacetylase inhibitors (HDACis) regulate the expression of numerous genes involved in cell death, apoptosis, and tumorigenesis. We found that the HDAC4/HDAC5 inhibitor LMK235 at 0.5 µM significantly reduced the cell viability and colony formation of patient-derived, temozolomide-resistant GBM P#5 TMZ-R, U-87 MG, and T98G cells. Moreover, LMK235 also significantly increased TUBA acetylation, which is an indicator of HDAC inhibition. Interestingly, LMK235 induced MAP1LC3 robust readout and puncta accumulation but did not enhance PARP1 cleavage or the proportion of annexin V-positive cells, suggesting that LMK235-induced cell death occurred via autophagy activation. Further RNA-seq analysis after LMK235 treatment showed that 597 different expression genes compared to control. After bioinformatic analysis by KEGG and STRING, we focused on 34 genes and validated their mRNA expression by qPCR. Further validation showed that 2 µM LMK235 significantly reduced the mRNA and protein expression of SCNN1A. Cell viability of SCNN1A-silenced cells were reduced, but cells were rescued while treated with an autophagy inhibitor bafilomycin A1. Conclusively, SCNN1A plays a role in LMK235-induced autophagy and cell death in GBM cells.

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Section: Discussionsupporting

confidence: 69%

A Selective Histone Deacetylase Inhibitor Induces Autophagy and Cell Death via SCNN1A Downregulation in Glioblastoma Cells

Chang

Tsai

et al. 2022

Cancers

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“…49,62 Furthermore, mechanical stimulation is an additional critical factor that synergistically promotes collagen assembly and maturation. 12 It has been shown that integrin-PI3K-Akt signaling and histone deacetylase 4 (HDAC4)-mediated nuclear signaling account for cytoskeleton deformation and cell differentiation caused by mechanical forces. 12 Taken together, the results suggest that the tenogenic microenvironment created by the TM-FAT initiates the differentiation of BMSCs and contributes to collagen assembly and maturation under mechanical stimulation.…”

Section: Resultsmentioning

confidence: 99%

“…9−12 It has been established that the fibers in such artificial tendons are crucial for the successful repair and regeneration of ruptured tendon tissue. 9,10,12 However, there are essential structural differences between FATs and natural tendons. 11 For instance, the fiber organization lacks the level of order seen in collagen fibers, and FATs often lack a collagen fiber-like structural hierarchy and effective interfiber bundling.…”

Section: Introductionmentioning

confidence: 99%

“…To replicate tissue-like biomechanical properties, various fiber manufacturing techniques such as weaving, yarn spinning, electrospinning, and melt electrowetting have been proposed for fibrous artificial tendons (FATs). − It has been established that the fibers in such artificial tendons are crucial for the successful repair and regeneration of ruptured tendon tissue. ,, However, there are essential structural differences between FATs and natural tendons . For instance, the fiber organization lacks the level of order seen in collagen fibers, and FATs often lack a collagen fiber-like structural hierarchy and effective interfiber bundling .…”

Section: Introductionmentioning

confidence: 99%

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Tendon Repair and Regeneration Using Bioinspired Fibrillation Engineering That Mimicked the Structure and Mechanics of Natural Tissue

Dong,

Peng,

Zhang

et al. 2023

ACS Nano

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Replicating the controlled nanofibrillar architecture of collagenous tissue represents a promising approach in the design of tendon replacements that have tissue-mimicking biomechanics�outstanding mechanical strength and toughness, defect tolerance, and fatigue and fracture resistance. Guided by this principle, a fibrous artificial tendon (FAT) was constructed in the present study using an engineering strategy inspired by the fibrillation of a naturally spun silk protein. This bioinspired FAT featured a highly ordered molecular and nanofibrillar architecture similar to that of soft collagenous tissue, which exhibited the mechanical and fracture characteristics of tendons. Such similarities provided the motivation to investigate FAT for applications in Achilles tendon defect repair. In vitro cellular morphology and expression of tendon-related genes in cell culture and in vivo modeling of tendon injury clearly revealed that the highly oriented nanofibrils in the FAT substantially promoted the expression of tendon-related genes combined with the Achilles tendon structure and function. These results provide confidence about the potential clinical applications of the FAT.

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“…However, these processes reduce the material’s biomechanical strength. After physical or chemical crosslinking, collagen scaffolds lose the mechanical properties of natural collagen tissues, and their degradation rate, in the long run, is increased [ 41 ].…”

Section: Products Of Tendon and Ligament Reconstruction And Repairmentioning

confidence: 99%

Functional biomaterials for tendon/ligament repair and regeneration

Tang

Wang

Xiang

et al. 2022

Regenerative Biomaterials

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With an increase in life expectancy and the popularity of high-intensity exercise, the frequency of tendon and ligament injuries has also increased. Owing to the specificity of its tissue, the rapid restoration of injured tendons and ligaments is challenging for treatment. This review summarises the latest progress in cells, biomaterials, active molecules, and construction technology in treating tendon/ligament injuries. The characteristics of supports made of different materials and the development and application of different manufacturing methods are discussed. The development of natural polymers, synthetic polymers, and composite materials has boosted the use of scaffolds. In addition, the development of electrospinning and hydrogel technology has diversified the production and treatment of materials. First, this paper briefly introduces the structure, function, and biological characteristics of tendons/ligaments. Then, it summarises the advantages and disadvantages of different materials, such as natural polymer scaffolds, synthetic polymer scaffolds, composite scaffolds, and ECM-derived biological scaffolds, in the application of tendon/ligament regeneration. We then discuss the latest applications of electrospun fiber scaffolds and hydrogels in regeneration engineering. Finally, we discuss the current problems and future directions in the development of biomaterials for restoring damaged tendons and ligaments.

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Synergistic effects of mechanical stimulation and crimped topography to stimulate natural collagen development for tendon engineering

Cited by 18 publications

References 48 publications

A Selective Histone Deacetylase Inhibitor Induces Autophagy and Cell Death via SCNN1A Downregulation in Glioblastoma Cells

A Selective Histone Deacetylase Inhibitor Induces Autophagy and Cell Death via SCNN1A Downregulation in Glioblastoma Cells

Tendon Repair and Regeneration Using Bioinspired Fibrillation Engineering That Mimicked the Structure and Mechanics of Natural Tissue

Functional biomaterials for tendon/ligament repair and regeneration

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