Synergistic Effect of Thymus vulgaris Essential Oil Oral Administration on Topically Treated Wound with Chitosan, Thyme Essential Oil and their Combination in Rats

Abstract

Herbal medication from natural resources plays an important role as antioxidant, antibacterial agent and demonstrating an inimitable property in wound curative rather than synthesized-toxic materials. The present investigation was designed to evaluate the potential healing efficacy of thyme (Thymus vulgaris) essential oil (TEO) oral adminstartion on topically treated wound with chitosan, TEO and their combination in vitro. Practically, effect of TEO oral administration on topically treated wounded rats in comparing to topical application of betadine for two weeks was carried out. Remarkably, treated group with TEO topically and orally exhibited the highest wound contractions among all treated groups. In Parallel, histopathological alteration was improved in TEO topically and orally applications whereas no degeneration, necrosis & inflammatory cells infiltration in skeletal muscle of wound with rejuvenation of hair follicles were observed. Wound healing efficiency was established in vivo and recommended as natural agent for wounds through protection of injury site from infections and inhibition of inflammatory cells as well as increasing connective tissue formation in repaired tissues. These favored results confirm that treated group with TEO topically combined with TEO orally have outstanding wound healing activity and need exploitation as an alternative source of natural wound agents for medical applications.

Ethnopharmacological Survey and Comparative Study of the Healing Activity of Moroccan Thyme Honey and Its Mixture with Selected Essential Oils on Two Types of Wounds on Albino Rabbits

“…The essential oil of Origanum vulgare has led to a significant reduction in the areas of wounds treated and showed an excellent capacity for tissue remodeling, particularly re-epithelialization [ 59 ]. Thymus vulgaris was recommended as a natural agent for wounds by protecting the wound site from infection, inhibiting inflammatory cells, and increasing the formation of connective tissue in repaired tissues [ 60 , 61 , 62 ].…”

“…Pires et al [ 79 ] also tested the addition of thymol to poly(dimethylsiloxane) on chitosan-alginate film. Since inflammation causes many difficulties, including infection, wound dehiscence, and impaired collagen synthesis, the anti-inflammatory effects of thymol would be promising material when thyme essential oil is used [ 60 ].…”

“…In harmony with our findings, the topical and systemic administration of thymol has shown remarkable anti-inflammatory activities in experimental models of edema and cutaneous inflammatory disorders [ 88 ], and the marked inhibitory effects of thymus oil therapy on wound-associated stasis, thrombosis, and tissue damage have been previously reported in burned wound models in rats [ 89 ]. Furthermore, the favored wound healing effects of oral and topical T. vulgaris EO therapy [ 60 ], or rosemary EO therapy [ 90 ], have been documented in surgically induced excision wound models in rats.…”

Hydrogel of Thyme-Oil-PLGA Nanoparticles Designed for Skin Inflammation Treatment

“…In fact, most clinical trials showed that herbal medicines significantly reduced acne lesions (both inflammatory and non-inflammatory), and were able to decrease acne severity (in mild-moderate acne) [4]. Among several natural compounds, thyme oil (THO) possesses excellent pharmaceutical properties [5,6]. THO is a product of the steam distillation of the fresh flowering aerial parts of Thymus vulgaris L. and/or Thymus zygis L. s, and it contains 37-55% thymol and 0.5-5.5% carvacrol [7].…”

Vesicular Nanocarriers for Phytocompounds in Wound Care: Preparation and Characterization

“…Herbal extracts (HEs) and phytocompounds (PCs) are the subject of considerable interest for their complex beneficial effects on skin health, such as regenerative, anti-inflammatory, antioxidant, and antimicrobial effects. During the last decade, among other versatile agents studied for wound healing, the plant-derived ingredients, also called phyto-drugs, have attracted great attention because they are safe, easily available, and affordable [ 8 , 9 , 10 , 11 ]. Despite their beneficial effects in wound healing, their bioavailability is reduced because of poor membrane permeability and instability issues.…”