Ethnopharmacological Survey and Comparative Study of the Healing Activity of Moroccan Thyme Honey and Its Mixture with Selected Essential Oils on Two Types of Wounds on Albino Rabbits

Abstract

Wound healing consists of several continuous phases involving various cells and chemical intermediates. As a rich source of nutrition elements, honey has proved to have potential benefits in the treatment of various diseases. The present study was designed to investigate the healing effect of a honey mixture with selected essential oils on chemical and thermal wound models in rabbits. Dressing mixtures of Thymus vulgaris honey with three essential oils (Origanum vulgare, Rosmarinus officinalis, and Thymus vulgaris) were prepared and applied daily in the treatment groups. These essential oils were rich in phytochemicals and had significant antibacterial activity against four selected ATCC bacterial strains. Madecasol ointment was used as a standard control. The healing effect of the mixtures was evaluated by measuring wound surface area and comparing healing time. The results showed that the healing rate in the treatment groups was significantly higher than that of the untreated group and standard group. The best healing effect for burns was seen in the mixture of honey and Thymus vulgaris essential oil, which had wound closure rates of 85.21% and 82.14% in thermal- and chemical-induced burns, respectively, and showed the shortest healing time (14 days) in comparison to other groups. Therefore, it can be concluded that honey mixtures have significant beneficial effects on skin wound healing and, thus, they may be used as a healing agent in different types of wounds in humans after specific clinical trials.

Antioxidant, Antidiabetic, and Antibacterial Potentials and Chemical Composition of Salvia officinalis and Mentha suaveolens Grown Wild in Morocco

“…The chemical components of SOEO and MSEO were determined by using gas chromatography/mass spectrometry (GC/MS) analysis conditions as described in our latterly published study [ 27 ].…”

“…The disc-diffusion test was employed to determine the preliminary antibacterial potential of SOEO and MSEO against the selected 10 bacterial pathogens, according to the protocol described previously by our research group [ 27 ]. Briefly, the tested EOs were mixed with 10% dimethyl sulfoxide (DMSO) to enhance their diffusion into the agar.…”

Determination of Volatile Compounds of Mentha piperita and Lavandula multifida and Investigation of Their Antibacterial, Antioxidant, and Antidiabetic Properties

“…The chemical components of Mentha piperita (MPEO) and Lavandula multifida (LMEO) essential oils were determined by using gas-chromatography/mass-spectrometry (GC/MS) analysis conditions as described in our latterly published study [ 22 ].…”

“…The disc diffusion test was used to assess the antibacterial potential of essential oils, with minor modifications to the procedure published previously [ 22 ]. To enhance diffusion in the culture medium, the examined essential oils were combined with dimethyl sulfoxide (DMSO) at a concentration of 5%.…”

Biological Properties of Latex, Aqueous Extracts and Bee Products of Euphorbia officinarum L.: A Short Review

“…echinus has also been reported in the treatment of wounds, skin infections and abscesses in diverse places in Morocco [ 16 , 17 , 18 ] and by the Sahrawi refugees in Algerian refugee camps [ 19 ]. Although Idm’hand et al [ 18 ] had noted the utilization of that species in the treatment of skin diseases, their work indicated that the specific treatment purpose of E. officinarum subsp. echinus was the elimination of helminths.…”