Synergistic effect of methylene blue and biogenic gold nanoparticles against Enterococcus faecalis

Maliszewska, Irena; Wróbel, Jakub; Wanarska, Ewelina; Podhorodecki, A.; Matczyszyn, Katarzyna

doi:10.1016/j.pdpdt.2019.05.042

Cited by 18 publications

(13 citation statements)

References 49 publications

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“…The articles included in the present systematic review and meta-analysis ranged in publication data from 2015 to 2022 [ 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 ] ( Table 1 ). Most of the studies have been performed in vitro studies.…”

Section: Resultsmentioning

confidence: 99%

“…Only one study also performed an animal model assay [ 41 ]. The most frequently inorganic nanoparticle evaluated was the metal type, with the same proportion for gold [ 29 , 34 , 38 ] and for silver [ 31 , 36 , 39 ]. The second most common was mesoporous silica [ 28 , 30 , 31 , 32 ], followed by metallic oxides [ 37 , 40 , 41 ] and then carbon quantum dots [ 33 , 35 ].…”

Section: Resultsmentioning

confidence: 99%

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Efficacy of Antimicrobial Photodynamic Therapy Mediated by Photosensitizers Conjugated with Inorganic Nanoparticles: Systematic Review and Meta-Analysis

et al. 2022

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Antimicrobial photodynamic therapy (aPDT) is a method that does not seem to promote antimicrobial resistance. Photosensitizers (PS) conjugated with inorganic nanoparticles for the drug-delivery system have the purpose of enhancing the efficacy of aPDT. The present study was to perform a systematic review and meta-analysis of the efficacy of aPDT mediated by PS conjugated with inorganic nanoparticles. The PubMed, Scopus, Web of Science, Science Direct, Cochrane Library, SciELO, and Lilacs databases were searched. OHAT Rob toll was used to assess the risk of bias. A random effect model with an odds ratio (OR) and effect measure was used. Fourteen articles were able to be included in the present review. The most frequent microorganisms evaluated were Staphylococcus aureus and Escherichia coli, and metallic and silica nanoparticles were the most common drug-delivery systems associated with PS. Articles showed biases related to blinding. Significant results were found in aPDT mediated by PS conjugated with inorganic nanoparticles for overall reduction of microorganism cultured in suspension (OR = 0.19 [0.07; 0.67]/p-value = 0.0019), E. coli (OR = 0.08 [0.01; 0.52]/p-value = 0.0081), and for Gram-negative bacteria (OR = 0.12 [0.02; 0.56/p-value = 0.0071). This association approach significantly improved the efficacy in the reduction of microbial cells. However, additional blinding studies evaluating the efficacy of this therapy over microorganisms cultured in biofilm are required.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Efficacy of Antimicrobial Photodynamic Therapy Mediated by Photosensitizers Conjugated with Inorganic Nanoparticles: Systematic Review and Meta-Analysis

et al. 2022

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“…Antimicrobial PDT has a number of unique advantages in dental pulp treatment. First, PDT shows better bactericidal effects on deeply embedded bacteria [31], especially with regard to biofilm destruction [14,32,33]. In addition, within a specific concentration range, the cytotoxicity of PDT is weaker than that of NaClO, which is the most commonly used root canal disinfectant [34].…”

Section: Discussionmentioning

confidence: 99%

Effects of Rose Bengal‐ and Methylene Blue‐Mediated Potassium Iodide‐Potentiated Photodynamic Therapy on Enterococcus faecalis: A Comparative Study

Yuan

Jia

et al. 2020

Lasers Surg Med

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Background and Objectives: This study was performed to compare the use of methylene blue (MB) and rose bengal (RB) in antimicrobial photodynamic therapy (PDT) targeting Enterococcus faecalis (E. faecalis) bacteria in planktonic and biofilm forms with potassium iodide (KI) potentiation. Study Design/Materials and Methods: E. faecalis bacteria in planktonic form were exposed to antimicrobial PDT protocols activating MB and RB, with or without KI potentiation, following laser irradiation with different exposure times, 60 mW/cm 2 laser power, and different photosensitizer agent (PS)/potentiator concentrations to observe relationships among the variables. Two continuous-wave diode lasers were used for irradiation (red light: λ = 660 nm and green light: λ = 565 nm). The pre-irradiation time was 10 minutes. The vitality of E. faecalis biofilm was assessed by confocal laser scanning microscopy, and the morphology was determined by scanning electron microscopy. The effects on the proliferation of stem cells from the apical papilla (SCAPs) were analyzed by cell counting kit-8 assay. The staining effect of antimicrobial PDT on dentin slices was investigated. Statistical analysis using a one-way analysis of variance was done. Results: KI-potentiated RB and MB antimicrobial PDT both effectively eradicated E. faecalis bacteria in planktonic and biofilm forms. The minimum bactericidal concentrations of PSs (±100 mM KI) were obtained through PDT on planktonic E. faecalis, and the optimal light parameters were 60 mW/cm 2 , 6 J/cm 2 for 100 seconds. KI-potentiated PDT effectively strengthened the ability to inhibit E. faecalis biofilm with 86.50 ± 5.78% for MB (P = 0.0015 < 0.01) and 91.50 ± 1.75% for RB (P = 0.0418 < 0.05) of bactericidal rate, with less toxicity for SCAPs (P < 0.001) and less staining. KI could reduce the staining induced by antimicrobial PDT on dentin slices. Conclusion: A combination of KI and antimicrobial PDT may be a useful alternative to conventional disinfection methods in endodontic treatment. MB and RB antimicrobial PDT at much lower concentrations with KI could hopefully achieve disinfection effects comparable with those of 1.5% NaClO while causing few adverse effects on SCAPs. KI helps to avoid staining problems associated with high concentrations of photosensitizer agents. Lasers Surg. Med.

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“…have also been evaluated in various studies, as such factors can modify their antibacterial activity [15,16]. Over the last years, various nanocarrier systems against E. faecalis have been reported such as ciprofloxacin and metronidazole encapsulated nanomatrix gels [17], bismuth nanoparticles [18], chitosan-propolis nanoparticles [19], doxycycline-functionalized polymeric nanoparticles [20], methylene blue and biogenic gold nanoparticles [21], silver nanoparticles and calcium hydroxide mixtures [22], biosynthesized silver nanoparticles [23], and graphene oxide nanoparticles [24].…”

Section: Introductionmentioning

confidence: 99%

Ciprofloxacin-Loaded Gold Nanoparticles against Antimicrobial Resistance: An In Vivo Assessment

Nawaz¹,

Ali²,

Rana³

et al. 2021

Nanomaterials

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Metallic nanoparticles, such as gold nanoparticles (AuNPs), have been extensively studied as drug delivery systems for various therapeutic applications. However, drug-loaded-AuNPs have been rarely explored in vivo for their effect on bacteria residing inside tissues. Ciprofloxacin (CIP) is a second-generation fluoroquinolone with a broad-spectrum of antibiotic properties devoid of developing bacteria resistance. This research is focused on the synthesis and physical characterization of Ciprofloxacin-loaded gold nanoparticles (CIP-AuNPs) and their effect on the colonization of Enterococcus faecalis in the liver and kidneys of mice. The successfully prepared CIP-AuNPs were stable and exerted enhanced in vitro antibacterial activity against E. faecalis compared with free CIP. The optimized CIP-AuNPs were administered (500 µg/Kg) once a day via tail vein to infected mice for eight days and were found to be effective in eradicating E. faecalis from the host tissues. Moreover, unlike CIP, CIP-AuNPs were non-hemolytic. In summary, this study demonstrated that CIP-AuNPs are promising and biocompatible alternative therapeutics for E.-faecalis-induced infections resistant to conventional drugs (e.g., beta-lactams and vancomycin) and should be further investigated.

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Synergistic effect of methylene blue and biogenic gold nanoparticles against Enterococcus faecalis

Cited by 18 publications

References 49 publications

Efficacy of Antimicrobial Photodynamic Therapy Mediated by Photosensitizers Conjugated with Inorganic Nanoparticles: Systematic Review and Meta-Analysis

Efficacy of Antimicrobial Photodynamic Therapy Mediated by Photosensitizers Conjugated with Inorganic Nanoparticles: Systematic Review and Meta-Analysis

Effects of Rose Bengal‐ and Methylene Blue‐Mediated Potassium Iodide‐Potentiated Photodynamic Therapy on Enterococcus faecalis: A Comparative Study

Ciprofloxacin-Loaded Gold Nanoparticles against Antimicrobial Resistance: An In Vivo Assessment

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