Ciprofloxacin-Loaded Gold Nanoparticles against Antimicrobial Resistance: An In Vivo Assessment

Nawaz, Afrah; Ali, Sarfraz; Rana, Nosheen Fatima; Tanweer, Tahreem; Batool, Amna; Webster, Thomas J; Menaa, Farid; Riaz, Sundus; Rehman, Zahra; Batool, Farhat; Fatima, Misha; Maryam, Tuba; Shafique, Iqra; Saleem, Abida; Iqbal, Arfa

doi:10.3390/nano11113152

Cited by 29 publications

(13 citation statements)

References 39 publications

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“…Accordingly, treatment with ciprofloxacin and thymol oils against Shigella flexneri reduced intestinal infiltration of inflammatory cells in male albino rats and thus reduced the severity of gastric ulcer [ 41 ]. Additionally, administration of ciprofloxacin-loaded gold nanoparticles decreased the load of Enterococcus faecalis in the liver and kidneys of mice and consequently reduced the severity of tissue damage [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Novel In Vivo Assessment of Antimicrobial Efficacy of Ciprofloxacin Loaded Mesoporous Silica Nanoparticles against Salmonella typhimurium Infection

et al. 2022

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Salmonella enterica serovar Typhimurium (S. typhimurium) is known for its intracellular survival, evading the robust inflammation and adaptive immune response of the host. The emergence of decreased ciprofloxacin (CIP) susceptibility (DCS) requires a prolonged antibiotic course with increased dosage, leading to threatening, adverse effects. Moreover, antibiotic-resistant bacteria can persist in biofilms, causing serious diseases. Hence, we validated the in vitro and in vivo efficacy of ciprofloxacin-loaded mesoporous silica nanoparticles (CIP–MSN) using a rat model of salmonella infection to compare the oral efficacy of 5 mg/kg body weight CIP–MSN and a traditional treatment regimen with 10 mg/kg CIP postinfection. Our results revealed that mesoporous silica particles can regulate the release rate of CIP with an MIC of 0.03125 mg/L against DCS S. typhimurium with a greater than 50% reduction of biofilm formation without significantly affecting the viable cells residing within the biofilm, and a sub-inhibitory concentration of CIP–MSN significantly reduced invA and FimA gene expressions. Furthermore, oral supplementation of CIP–MSN had an insignificant effect on all blood parameter values as well as on liver and kidney function parameters. MPO and NO activities that are key mediators of oxidative stress were abolished by CIP–MSN supplementation. Additionally, CIP–MSN supplementation has a promising role in attenuating the elevated secretion of pro-inflammatory cytokines and chemokines in serum from S. typhimurium-infected rats with a reduction in pro-apoptotic gene expression, resulting in reduced S. typhimurium-induced hepatic apoptosis. This counteracted the negative effects of the S. typhimurium challenge, as seen in a corrected histopathological picture of both the intestine and liver, along with increased bacterial clearance. We concluded that, compared with a normal ciprofloxacin treatment regime, MSN particles loaded with a half-dose of ciprofloxacin exhibited controlled release of the antibiotic, which can prolong the antibacterial effect.

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Section: Discussionmentioning

confidence: 99%

Novel In Vivo Assessment of Antimicrobial Efficacy of Ciprofloxacin Loaded Mesoporous Silica Nanoparticles against Salmonella typhimurium Infection

et al. 2022

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“…Drug-loaded AuNPs could collapse bacteria membrane potential, hinder ATPase activity, and halt binding of ribosomes to tRNA leading to deteriorated bacterial cell metabolism [ 159 ]. Antibiotic ciprofloxacin (CIP) is widely used for the treatment of numerous bacterial infections in joints, bones, skin, tooth, gastrointestinal, and urinary and respiratory tracts [ 160 ]. Recently, Ciprofloxacin-loaded gold nanoparticles (CIP-AuNPs) were prepared [ 161 ].…”

Section: Antimicrobial Nanomedicine Drug Delivery Systemsmentioning

confidence: 99%

Recent Advances in Antimicrobial Nano-Drug Delivery Systems

et al. 2022

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Infectious diseases are among the major health issues of the 21st century. The substantial use of antibiotics over the years has contributed to the dissemination of multidrug resistant bacteria. According to a recent report by the World Health Organization, antibacterial (ATB) drug resistance has been one of the biggest challenges, as well as the development of effective long-term ATBs. Since pathogens quickly adapt and evolve through several strategies, regular ATBs usually may result in temporary or noneffective treatments. Therefore, the demand for new therapies methods, such as nano-drug delivery systems (NDDS), has aroused huge interest due to its potentialities to improve the drug bioavailability and targeting efficiency, including liposomes, nanoemulsions, solid lipid nanoparticles, polymeric nanoparticles, metal nanoparticles, and others. Given the relevance of this subject, this review aims to summarize the progress of recent research in antibacterial therapeutic drugs supported by nanobiotechnological tools.

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“…Among these, S. aureus is the most frequently isolated bacterial population from implant-associated infections [56]. Enterococci, including E. faecalis, are the second most frequently isolated Gram-positive bacteria [57].…”

Section: Biological Assaysmentioning

confidence: 99%

Implant Surfaces Containing Bioglasses and Ciprofloxacin as Platforms for Bone Repair and Improved Resistance to Microbial Colonization

et al. 2022

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Coatings are an attractive and challenging selection for improving the bioperformance of metallic devices. Composite materials based on bioglass/antibiotic/polymer are herein proposed as multifunctional thin films for hard tissue implants. We deposited a thin layer of the polymeric material by matrix-assisted pulsed laser evaporation—MAPLE onto Ti substrates. A second layer consisting of bioglass+antibiotic was applied by MAPLE onto the initial thin film. The antimicrobial activity of MAPLE-deposited thin films was evaluated on Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Pseudomonas aeruginosa standard strains. The biocompatibility of obtained thin films was assessed on mouse osteoblast-like cells. The results of our study revealed that the laser-deposited coatings are biocompatible and resistant to microbial colonization and biofilm formation. Accordingly, they can be considered viable candidates for biomedical devices and contact surfaces that would otherwise be amenable to contact transmission.

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Ciprofloxacin-Loaded Gold Nanoparticles against Antimicrobial Resistance: An In Vivo Assessment

Cited by 29 publications

References 39 publications

Novel In Vivo Assessment of Antimicrobial Efficacy of Ciprofloxacin Loaded Mesoporous Silica Nanoparticles against Salmonella typhimurium Infection

Novel In Vivo Assessment of Antimicrobial Efficacy of Ciprofloxacin Loaded Mesoporous Silica Nanoparticles against Salmonella typhimurium Infection

Recent Advances in Antimicrobial Nano-Drug Delivery Systems

Implant Surfaces Containing Bioglasses and Ciprofloxacin as Platforms for Bone Repair and Improved Resistance to Microbial Colonization

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