Synergistic effect of BAP and GA3 on in vitro flowering of Guizotia abyssinica Cass.-A multipurpose oil crop

Baghel, Sapna; Bansal, Y. K.

doi:10.1007/s12298-014-0229-3

Cited by 10 publications

(4 citation statements)

References 27 publications

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“…Superior effect of BAP for shoot multiplication has been reported among various medicinal plants of Asteraceae, like Tanacetum cinerariifolium (Hedayat et al 2009), Spilanthes acmella (Saritha and Naidu 2008), Launaea sarmentosa (Mahesh et al 2012). As in the present study, the reduction of shoot regeneration potential by the combined effect of BAP and GA 3 compared to a medium with cytokinin alone was also demonstrated in Guizotia abyssinica (Baghel and Bansal 2014). On the contrary, a combination of BAP and GA 3 had shown better response in Artimisia vulgaris (Borzabad et al 2010).…”

Section: Resultssupporting

confidence: 77%

In vitro Regeneration of Blepharispermum subsessile DC: An Endangered Medicinal Plant of Odisha, India using Cotyledon Explants

Nayak

Kalidass

2016

Plant Tissue Cult. & Biotech.

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Multiple shoots were induced on cotyledon explants of in vitro grown seedlings of Blepharispermum subsessile DC, cultured on MS medium supplemented with various combinations and concentrations of BAP, IBA and GA 3 . The highest regenerative response was observed on medium containing 2.5 mg/l BAP where shoot buds initiated after 12 days of inoculation and about 32 shoots were produced in 30 days time. Addition of GA 3 played a key role in leaf expansion and elongation of shoot buds. Addition of the auxin IBA to the induction medium resulted in more callus proliferation rather than shoot bud induction. The elongated shoots were transferred to root induction medium consisting of half strength MS supplemented with IAA, NAA and IBA. Highest rooting response (90%) was recorded in ½ MS supplemented with 1.0 mg/l IAA. Acclimatized plants were maintained in polybags with garden soil for future reintroduction program to their natural habitat.

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Section: Resultssupporting

confidence: 77%

In vitro Regeneration of Blepharispermum subsessile DC: An Endangered Medicinal Plant of Odisha, India using Cotyledon Explants

Nayak

Kalidass

2016

Plant Tissue Cult. & Biotech.

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“…In addition, it is also able to increase the number of shoots from axillary buds [38], similar to that observed in the present experiment with Phalaenopsis. Similarly, the combination of 6-BA with GA 3 was able to induce faster in vitro shoot production and increased the mean number of in vitro shoots in Guizotia abyssinica [39].…”

Section: Effects Of Phytoregulators On In Vitro Development Of Phalae...mentioning

confidence: 86%

Micropropagation from Inflorescence Nodal Segments of Phalaenopsis and Acclimatization of Plantlets Using Different Substrates

et al. 2022

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Phalaenopsis is an orchid genus of great economic value in world floriculture. In vitro clonal propagation is the only large-scale feasible method for Phalaenopsis propagation, but it is difficult because of the low multiplication rate. The aim of this study was to evaluate the effect of types and concentrations of N6-benzyladenine (6-BA) and gibberellic acid (GA3) on the in vitro multiplication of shoots from inflorescence nodal segments (INS) of Phalaenopsis hybrids. INS with one axillary bud were inoculated in New Dogashima Medium with different combinations of BA and GA3. The results show that the treatment containing 1.0 mg L−1 BA and 1.5 mg L−1 GA3 showed the higher percentage of live inflorescence segments (71.48%) and a number of shoots (1.68 shoots/INS). The highest 6-BA concentration (4 mg L−1) tested in this study resulted in the best shoot multiplication rate (4.3). Contamination and browning of the INS tissues were the main difficulties identified for clonal propagation of Phalaenopsis. Successful in vitro rooting occurred on half-strength Murashige and Skoog medium (100%), and acclimatization (100%) was obtained independent of the substrates. However, the best gains in number of roots, leaves, chlorophyll content, and fresh weight of plantlets were achieved using vermiculite.

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“…Previously, cytokinin-induced in vitro flowering was reported in Anethum graveolens ( Jana and Shekhawat, 2011 ), Swertia chirayita ( Sharma et al, 2014 ), and Guizotia abyssinica ( Baghel and Bansal, 2014 ). The impact of the synergistic effect of endogenous auxin and cytokinin facilitates in vitro flowering at the species level in Micrococca mercurialis ( Jeyachandran and Bastin, 2013 ), Withania ( Saritha and Naidu, 2007 ), Ocimum basilicum ( Sudhakaran and Sivasankari, 2002 ), Kniphofia leucocephala ( Taylor et al, 2005 ), and Vitex negundo ( Thiruvengadam and Jayabalan, 2001 ).…”

Section: Discussionmentioning

confidence: 99%

Differential Gene Expression and Withanolides Biosynthesis During in vitro and ex vitro Growth of Withania somnifera (L.) Dunal

et al. 2022

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Ashwagandha (Withania somnifera L. Dunal) is a medicinally important plant with withanolides as its major bioactive compounds, abundant in the roots and leaves. We examined the influence of plant growth regulators (PGRs) on direct organogenesis, adventitious root development, withanolide biosynthetic pathway gene expression, withanolide contents, and metabolites during vegetative and reproductive growth phases under in vitro and ex vitro conditions. The highest shooting responses were observed with 6-benzylaminopurine (BAP) (2.0 mg L–1) + Kinetin (KIN) (1.5 mg L–1) supplementation. Furthermore, BAP (2.0 mg L–1) + KIN (1.5 mg L–1) + gibberellic acid (GA3) (0.5 mg L–1) exhibited better elongation responses with in vitro flowering. Half-strength MS medium with indole-3-butyric acid (IBA) (1.5 mg L–1) exhibited the highest rooting responses and IBA (1.0 mg L–1) with highest fruits, and overall biomass. Higher contents of withaferin A (WFA) [∼8.2 mg g–1 dry weight (DW)] were detected in the reproductive phase, whereas substantially lower WFA contents (∼1.10 mg g–1 DW) were detected in the vegetative phase. Cycloartenol synthase (CAS) (P = 0.0025), sterol methyltransferase (SMT) (P = 0.0059), and 1-deoxy-D-xylulose-5-phosphate reductase (DXR) (P = 0.0375) genes resulted in a significant fold change in expression during the reproductive phase. The liquid chromatography-mass spectrometry (LC-MS) analysis revealed metabolites that were common (177) and distinct in reproductive (218) and vegetative (167) phases. Adventitious roots cultured using varying concentrations of indole-3-acetic acid (IAA) (0.5 mg L–1) + IBA (1.0 mg L–1) + GA3 (0.2 mg L–1) exhibited the highest biomass, and IAA (0.5 mg L–1) + IBA (1.0 mg L–1) exhibited the highest withanolides content. Overall, our findings demonstrate the peculiarity of withanolide biosynthesis during distinct growth phases, which is relevant for the large-scale production of withanolides.

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Synergistic effect of BAP and GA3 on in vitro flowering of Guizotia abyssinica Cass.-A multipurpose oil crop

Cited by 10 publications

References 27 publications

In vitro Regeneration of Blepharispermum subsessile DC: An Endangered Medicinal Plant of Odisha, India using Cotyledon Explants

In vitro Regeneration of Blepharispermum subsessile DC: An Endangered Medicinal Plant of Odisha, India using Cotyledon Explants

Micropropagation from Inflorescence Nodal Segments of Phalaenopsis and Acclimatization of Plantlets Using Different Substrates

Differential Gene Expression and Withanolides Biosynthesis During in vitro and ex vitro Growth of Withania somnifera (L.) Dunal

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