Micropropagation from Inflorescence Nodal Segments of Phalaenopsis and Acclimatization of Plantlets Using Different Substrates

Abstract

Phalaenopsis is an orchid genus of great economic value in world floriculture. In vitro clonal propagation is the only large-scale feasible method for Phalaenopsis propagation, but it is difficult because of the low multiplication rate. The aim of this study was to evaluate the effect of types and concentrations of N6-benzyladenine (6-BA) and gibberellic acid (GA3) on the in vitro multiplication of shoots from inflorescence nodal segments (INS) of Phalaenopsis hybrids. INS with one axillary bud were inoculated in New Dogashima Medium with different combinations of BA and GA3. The results show that the treatment containing 1.0 mg L−1 BA and 1.5 mg L−1 GA3 showed the higher percentage of live inflorescence segments (71.48%) and a number of shoots (1.68 shoots/INS). The highest 6-BA concentration (4 mg L−1) tested in this study resulted in the best shoot multiplication rate (4.3). Contamination and browning of the INS tissues were the main difficulties identified for clonal propagation of Phalaenopsis. Successful in vitro rooting occurred on half-strength Murashige and Skoog medium (100%), and acclimatization (100%) was obtained independent of the substrates. However, the best gains in number of roots, leaves, chlorophyll content, and fresh weight of plantlets were achieved using vermiculite.

Micropropagation and Genetic Fidelity of Fegra Fig (Ficus palmata Forssk.) and Grafting Compatibility of the Regenerated Plants with Ficus carica

“…Cytokinin is well-known for its ability to promote shoot multiplication by acting as a signaling molecule involved directly in the cell division of meristematic cells [43]. They regulate apical dominance, the size of the shoot meristem, the amount of leaf primordia, shoot growth, leaf production, and the development of axillary buds [44].…”

Morpho‐histology of co‐occurrence of somatic embryos, shoots, and inflorescences within a callus of Limonium ‘Misty Blue’

“…Though it is uncommon, in many instances inflorescences were used for in vitro cloning of herbaceous plants like Phalaenopsis sp. (Zanello et al, 2022), garlic (Wen et al, 2020; Bhusal et al, 2021), Cenchrus ciliaris (Goyal et al, 2023), Artemisia annua (Wang et al, 2016), Eremochloa ophiuroides (Ma et al, 2018), and Cannabis sativa (Piunno et al, 2019).…”