Synergistic biofilm formation byTreponema denticolaandPorphyromonas gingivalis

Abstract

Biofilm formation is an important step in the etiology of periodontal diseases. In this study, in vitro biofilm formation by Treponema denticola and Porphyromonas gingivalis 381 displayed synergistic effects. Confocal microscopy demonstrated that P. gingivalis attaches to the substratum first as a primary colonizer followed by coaggregation with T. denticola to form a mixed biofilm. The T. denticola flagella mutant as well as the cytoplasmic filament mutant were shown to be essential for biofilm formation as well as coaggregation with P. gingivalis. The major fimbriae and Arg-gingipain B of P. gingivalis also play important roles in biofilm formation with T. denticola.

Oral colonization of Gram-negative anaerobes as a risk factor for preterm delivery

“…199 Bacterial co-aggregations in biofilms such as plaque are common, and microbial interactions may modulate their pathogenic potential. Examples are P. gingivalis, T. denticola, T. forsythia, F. nucleatum, Aa and Campylobacter, [200][201][202][203] resulting in the production of excessive inflammatory responses. periodontal disease in pregnant women in an effort to prevent adverse pregnancy outcomes, while recent studies have indicated that the BANA test may be a useful screening tool in identifying mothers at risk for PTB 13,26,73 and would be worth exploring.…”

Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

“…Furthermore, immunohistochemical techniques have shown that P. gingivalis is localized beneath T. denticola in human subgingival plaque, while in deeper subgingival plaque their coexistence has been observed (Kigure et al, 1995). These various observations have been reproduced in dual-species biofilm experiments, which show that P. gingivalis acts as an initial colonizer of the substratum, with subsequent incorporation of T. denticola (Yamada et al, 2005). Interactions between these bacteria have been suggested to be mediated by P. gingivalis fimbriae recognizing dentilisin (Hashimoto et al, 2003) or by gingipains (Ito et al, 2010), and to involve the major surface protein (Msp) of T. denticola (Vesey & Kuramitsu, 2004;Rosen et al, 2008).…”

In vitro Antibacterial Activity of Zingiber officinale and Orthosiphon stamineus on Enterococcus faecalis

“…Biofilm formation by E. faecalis was quantified according to Yamada et al (2005) ly. The ampici shows attach bacterial cells ell attachment a and 3b).…”