Synergistic antibacterial and antibiofilm effects of ultrasound and MEL-A against methicillin-resistant Staphylococcus aureus

The increasing numbers of infections caused by multidrug-resistant (MDR) pathogens highlight the urgent need for new alternatives to conventional antibiotics. Antimicrobial peptides have the potential to be promising alternatives to antibiotics because of their effective bactericidal activity and highly selective toxicity. The present study was conducted to investigate the antibacterial, antibiofilm, and anti-adhesion activities of different CTP peptides (CTP: the original hybrid peptide cathelicidin 2 (1-13)-thymopentin (TP5); CTP-NH2: C-terminal amidated derivative of cathelicidin 2 (1-13)-TP5; CTPQ: glutamine added at the C-terminus of cathelicidin 2 (1-13)-TP5) by determining the minimal inhibitory concentrations (MICs), minimal bactericidal concentrations (MBCs), propidium iodide uptake, and analysis by scanning electron microscopy, transmission electron microscopy, and confocal laser scanning microscopy). The results showed that CTPs had broad-spectrum antibacterial activity against different gram-positive and gram-negative bacteria, with MICs against the tested strains varying from 2 to 64 μg/mL. CTPs at the MBC (2 × MIC 64 μg/mL) showed strong bactericidal effects on a standard methicillin-resistant Staphylococcus aureus strain ATCC 43300 after co-incubation for 6 h through disruption of the bacterial membrane. In addition, CTPs at 2 × MIC also displayed effective inhibition activity of several S. aureus strains with a 40–90% decrease in biofilm formation by killing the bacteria embedded in the biofilms. CTPs had low cytotoxicity on the intestinal porcine epithelial cell line (IPEC-J2) and could significantly decrease the rate of adhesion of S. aureus ATCC 43300 on IPEC-J2 cells. The current study proved that CTPs have effective antibacterial, antibiofilm, and anti-adhesion activities. Overall, this study contributes to our understanding of the possible antibacterial and antibiofilm mechanisms of CTPs, which might be an effective anti-MDR drug candidate.

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“…The assay was performed as reported by Shu et al [61]. The preliminary growth and CTP treatment was the same as for the time-kill curves.…”

Section: Enumeration Of Viable S Aureusmentioning

confidence: 99%

Novel Hybrid Peptide Cathelicidin 2 (1-13)-Thymopentin (TP5) and Its Derived Peptides with Effective Antibacterial, Antibiofilm, and Anti-Adhesion Activities

Guo

Tong

Wang

et al. 2021

IJMS

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“…Li et al, (2021) reported that the leakage of nucleic acids and protein in Rhizopus stolonifer after treatment with US combined with slightly acid electrolytic water was significantly higher than the individual treatment [33] . In fact, US could damage the outer cell membrane, which could help CA penetrate into the membrane and cell wall, thereby enhancing the ability of CA to damage the cell membrane and cause the leakage of ATP and nucleic acids [35] , [36] .…”

Section: Resultsmentioning

confidence: 99%

The combination of ultrasound and chlorogenic acid to inactivate Staphylococcus aureus under planktonic, biofilm, and food systems

Sun

Wang

Sun

et al. 2021

Ultrasonics Sonochemistry

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Highlights The synergistic bactericidal effect had produced by ultrasound (US) and chlorogenic acid (CA). US plus CA changed the morphology of S. aureus cells.. US plus CA led the leakage of internal constituents. US plus CA reduced the concentration of polysaccharide in biofilms. US plus CA reduced S. aureus in mutton by 1.14 log CFU/g.

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“…As the above mentioned study found, ultrasound usually only results in bacterial inhibition and partial sterilization; this cannot effectively ensure the safety of food and has limited the application of ultrasound in food processing. However, several recent studies have found that ultrasound, in combination with other bacteriostatic agents, could enhance the disinfection of Staphylococcus aureus, Salmonella typhimurium , and other bacteria [9] , [27] . Therefore, further study to confirm the inhibitory effect of HIU combined with antibacterial agents on B. subtilis is of value.…”

Section: Discussionmentioning

confidence: 99%

Bacteriostatic effects of high-intensity ultrasonic treatment on Bacillus subtilis vegetative cells

Luo

Wang

et al. 2021

Ultrasonics Sonochemistry

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Synergistic antibacterial and antibiofilm effects of ultrasound and MEL-A against methicillin-resistant Staphylococcus aureus

Cited by 36 publications

References 53 publications

Novel Hybrid Peptide Cathelicidin 2 (1-13)-Thymopentin (TP5) and Its Derived Peptides with Effective Antibacterial, Antibiofilm, and Anti-Adhesion Activities

Novel Hybrid Peptide Cathelicidin 2 (1-13)-Thymopentin (TP5) and Its Derived Peptides with Effective Antibacterial, Antibiofilm, and Anti-Adhesion Activities

The combination of ultrasound and chlorogenic acid to inactivate Staphylococcus aureus under planktonic, biofilm, and food systems

Bacteriostatic effects of high-intensity ultrasonic treatment on Bacillus subtilis vegetative cells

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