Bacteriostatic effects of high-intensity ultrasonic treatment on Bacillus subtilis vegetative cells

Abstract

Graphical abstract

Unravelling the Polytoxicology of Chlorfenapyr on Non-Target HepG2 Cells: The Involvement of Mitochondria-Mediated Programmed Cell Death and DNA Damage

“…They are also acknowledged as the main source of ROS, mainly originating from the respiratory chain [ 14 ]. Various environmental stressors, such as pesticides, are known to enhance ROS production and disturb cellular redox homeostasis [ 15 , 16 , 17 , 18 ]. Our data confirmed that CHL treatment caused rapid ROS accumulation in HepG2 cells.…”

Ultrasound-assisted pH-shifting remodels egg-yolk low-density lipoprotein to enable construction of a stable aqueous solution of vitamin D3

“…For scanning electron microscopy (SEM), samples were diluted to 0.05 mg/mL with deionized water of the corresponding pH value. Diluted samples (10 μL) were added to clean slides, air-dried for 24 h, and then observed with a scanning electron microscope (Apreo 2C; Thermo Fisher Scientific, Waltham, MA, USA) at an acceleration voltage of 10 kV ( Luo et al, 2021 ; Wang et al, 2022 ).…”

Quantitative proteomic analysis provides insight into the survival mechanism of Salmonella typhimurium under high-intensity ultrasound treatment

“…Untreated and HIU-treated (US-270, US-90, US-360) bacterial cells were centrifuged at 6790× g for 10 min at 4°C, rinsed in PBS (pH 7.2) buffer three times, and then stored at −80°C (cryopreservation) ( Li, et al, 2020 ; Luo et al, 2021 ). The collected Salmonella cells were lysed by the lysis solution (pH 8.1, 100 mM Tris-HCl, 1.5% SDS) and the total protein was precipitated through acetone.…”