Synchronous Fluorescence Spectroscopy and Its Applications in Clinical Analysis and Food Safety Evaluation

Li, Yao‐Qun; Li, Xiuying; Shindi, Ali Abbas Falih; Zou, Zheguang; Liu, Qian; Lin, Li-Rong; Li, Na

doi:10.1007/978-1-4419-9828-6_5

Cited by 45 publications

(37 citation statements)

References 38 publications

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“…Synchronous fluorescence spectroscopy can provide the microenvironment information on tyrosine and tryptophan residues . As shown in Figure S2, synchronous fluorescence spectra represent the information of tryptophan and tyrosine residues, respectively, when the wavelength interval (Δ λ ) of the excitation and emission wavelengths is fixed at 60 and 15 nm, respectively.…”

Section: Resultsmentioning

confidence: 98%

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The binding interaction between cadmium‐based, aqueous‐phase quantum dots with Candida rugosa lipase

Zhao

Meng

et al. 2018

J of Molecular Recognition

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As a promising biolabeling biomaterials, quantum dots (QDs) present a great potential. However, the toxicity of QDs to organisms has attracted wide attention. In our research, we introduced an in vitro method to study the molecular mechanisms for the structure and activity alterations of Candida rugosa lipase (CRL) with the binding of 3-mercaptopropionic acid-capped CdTe QDs. Multiple spectroscopic methods, isothermal titration calorimetry, and enzyme activity measurements were used in this paper. QDs statically quenched the intrinsic fluorescence of CRL with the quenching constant decreases from 2.46 × 10 to 1.64 × 10 L mol second (298 to 310 K). It binds to CRL through hydrophobic force with 1 binding site, unfolding and loosening the skeleton and changed its secondary structure. Rather than aggregating on the surface, it enters the pocket of the CRL to interact with Ser-209 (2.43 Å) and the residues surrounding Ser-209, making the catalytic triad more exposed. Furthermore, the activity of CRL was inhibited by approximately 15%. This work demonstrates that 3-mercaptopropionic acid-capped CdTe QDs may cause negative effects to CRL and obtains a molecular mechanism on QD-induced toxicity to proteins in vitro.

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Section: Resultsmentioning

confidence: 98%

“…This result indicates that MPA‐capped CdTe QDs lead to the unfolding and loosening of the CRL skeleton. The hydrophobic region of CRL began to spread to the more hydrophilic region and the pole of CRL solution increases, which may result in the refolding and movement of the surface loop (lid) of CRL and influence the activation of CRL …”

Section: Resultsmentioning

confidence: 99%

The binding interaction between cadmium‐based, aqueous‐phase quantum dots with Candida rugosa lipase

Zhao

Meng

et al. 2018

J of Molecular Recognition

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“…Synchronous fluorescence spectroscopy (SFS) is a superior technique over conventional fluorescence spectroscopy by its advantages such as narrowing of the spectral peaks, simplification of spectra, reduction of light scattering and improvement of selectivity …”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of amlodipine and metoprolol in their combined dosage form using a synchronous fluorescence spectrofluorimetric method

et al. 2017

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Highly sensitive, rapid, accurate and precise synchronous fluorescence spectrofluorimetric method has been developed for simultaneous analysis of a mixture of amlodipine (AMD) and metoprolol (MET). The method relies on measuring the relative synchronous fluorescence intensity of both drugs at Δλ of 90 nm in acetate buffer solution at pH 5. The experimental parameters influencing the developed method were investigated and optimized. The method was linear over the ranges 0.2-2 μg/ml and 0.5-10 μg/ml for AMD and MET, respectively. The limits of detection were 50 ng/ml for AMD and 130 ng/ml for MET while the limits of quantitation were 150 ng/ml for AMD and 390 ng/ml for MET. The developed method was applied successfully for the determination of the two drugs in their co-formulated tablet. The mean percent recoveries were found to be 100.51 and 99.57 for AMD and MET, respectively.

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“…Devido as características estruturais dessa molécula (presença de anéis aromáticos com elétrons altamente conjugados) o BFA apresenta alto potencial para a determinação por fluorescência CHI, et al, 2015;SYED, et al, 2011). Técnicas como a espectroscopia de fluorescência (fluorescência sincronizada) apresentam razoável sensibilidade, são de relativo baixo custo e de simples operação, quando comparadas ao LC-MS/MS, e podem ser utilizadas como alternativas para a determinação simultânea de múltiplos analitos devido às suas características, como estreitamento da banda espectral, simplificação do espectro de emissão e contração da faixa espectral (LI et al, 2010).…”

Section: Bisfenol Sunclassified

“…Entretanto, esta técnica não tem sido utilizada em amostras complexas, para a determinação de múltiplos analitos, devido aos problemas de sobreposição dos espectros de excitação e/ou emissão, necessitando de um procedimento de pré-separação antes da análise da amostra (LI et al, 2010;PATRA et al, 2002). Isso pode ser resolvido utilizando a fluorescência sincronizada, onde a sobreposição dos espectros pode ser minimizada.…”

Section: Fluorescência Sincronizadaunclassified

Desenvolvimento de um método simples e rápido para determinação de bisfenol A em amostras de papéis por espectroscopia de fluorescência sincronizada

Gallimberti¹

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Bisphenol A (BPA) is used in a variety of consumer products, including some paper products, particularly thermal receipt papers, for which it is used as a color developer. Nevertheless, little is known about the magnitude of BPA contamination or human exposure to BPA as a result of contact with papers. In this study, was develop and validate an methodology for determination of BPA by synchronous fluorescence spectroscopy. The developed and validated method was fast, easy, low cost, with a good sensibility and appropriate for application in paper samples. The method was linear on the range of to 25 a 450 µg.L -1 (r = 0,9993). The detection and quantification limits were 1,20 and 3,99 µg.L -1 respectively. Inter and intraday precision were between 3,21 to 8,54% and 0,29 to 3,92%, respectively. Accuracy was between 87,4 to 111%.The recovery was between 100 to 102 % (extracted samples). The validated methodology was applied in the BPA determination in 13 types pf paper products (n=444), including thermal receipt papers (n=341), lottery tickets (n=7), bus tickets (n=5), business cards (n=12), mailing envelopes (n=11), flyers (n=10), napkins (n=8), printing paper (n=3), food contact paper (n=20), kitchen rolls (n=4), toilet paper (n=8), newspaper (n=6), and magazines (n=9). BPA was found in 98% of thermal receipt papers (n = 341) at concentrations ranging from below the limit of quantitation (LOQ) to 27,7 mg.g -1 with a geometric mean of 14,6 mg.g -1 and a median of 17,7 mg.g -1 . The majority (96%) of other paper products (n = 103) contained BPA at concentrations ranging from below the LOQ to 379 µg.g -1 with a geometric mean of 14,7 µg.g -1 and a median of 17,3 µg.g -1 . Whereas thermal receipt papers contained the highest concentrations of BPA (milligram-per-gram), the others types of papers, especially those produced from recycled paper contain lower concentrations of BPA (microgramper-gram). Estimation of human exposure to BFA through manipulation of different paper types was calculated based on the measure of concentration and frequency of manipulation. The estimated daily intake for BFA adjusted body weight (calculated at median concentrations) through dermal absorption from handling of papers was 14.5and 1070 ng/day for the general population and occupationally exposed individuals, iv respectively.

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Synchronous Fluorescence Spectroscopy and Its Applications in Clinical Analysis and Food Safety Evaluation

Cited by 45 publications

References 38 publications

The binding interaction between cadmium‐based, aqueous‐phase quantum dots with Candida rugosa lipase

The binding interaction between cadmium‐based, aqueous‐phase quantum dots with Candida rugosa lipase

Simultaneous determination of amlodipine and metoprolol in their combined dosage form using a synchronous fluorescence spectrofluorimetric method

Desenvolvimento de um método simples e rápido para determinação de bisfenol A em amostras de papéis por espectroscopia de fluorescência sincronizada

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