Simultaneous determination of amlodipine and metoprolol in their combined dosage form using a synchronous fluorescence spectrofluorimetric method

Abstract

Highly sensitive, rapid, accurate and precise synchronous fluorescence spectrofluorimetric method has been developed for simultaneous analysis of a mixture of amlodipine (AMD) and metoprolol (MET). The method relies on measuring the relative synchronous fluorescence intensity of both drugs at Δλ of 90 nm in acetate buffer solution at pH 5. The experimental parameters influencing the developed method were investigated and optimized. The method was linear over the ranges 0.2-2 μg/ml and 0.5-10 μg/ml for AMD and MET, respectively. The limits of detection were 50 ng/ml for AMD and 130 ng/ml for MET while the limits of quantitation were 150 ng/ml for AMD and 390 ng/ml for MET. The developed method was applied successfully for the determination of the two drugs in their co-formulated tablet. The mean percent recoveries were found to be 100.51 and 99.57 for AMD and MET, respectively.

Electrochemical sensing of dobutamine, paracetamol, amlodipine, and daclatasvir in serum based on thiourea SAMs over nano-gold particles–CNTs composite

“…10,11,19,20,78–82 Also, the TU-SAMs sensor exhibits low DLs for DB, PA, AM, and DAC determination compared to other reported methods in the literature, Table S2 (ESI†). 8,9,29,30,35,36,43,44…”

“…10,11,19,20,[78][79][80][81][82] Also, the TU-SAMs sensor exhibits low DLs for DB, PA, AM, and DAC determination compared to other reported methods in the literature, Table S2 (ESI †). 8,9,29,30,35,36,43,44 Thus, the synergistic effect between all the modifiers of the composite; CNTs, Au nano and TU, enhances the kinetic process for electro-oxidation of the studied drugs.…”

“…34 Different methods were reported for the determination of AM such as HPLC 35 and synchronous fluorescence spectrofluorometric methods. 36 PA is used to treat pain, flu, colds and reduce fever. 37 It is a common drug taken to decrease or kill pain and it is always taken with other drugs during the treatment period.…”

“…34 Different methods were reported for the determination of AM such as HPLC 35 and synchronous fluorescence spectrofluorometric methods. 36…”

Spectrofluorimetric assay of amlodipine and perindopril in their raw materials, pharmaceutical formulations and spiked human plasma through the formation of complexes with Eosin Y

“…1-a). Various analytical techniques have been reported for determination of AML, either alone or in combination with other drugs, including, spectrophotometric techniques (Attimarad et al 2019;Erden et al 2016;Hegazy et al 2015;Karadurmuş et al 2018;Khachornsakkul and Dungchai 2020;Mahant et al 2016;Saad et al 2016;Siridevi et al 2019), spectrofluorimetric techniques (Attala et al 2020;Dinç et al 2017;El-Kosasy et al 2015;Ibrahim et al 2015;Mabrouk et al 2018;Mohamed et al 2016;Mohammed 2015;Nasr and Shalan 2020;Sh Shalan et al 2015;Shereen Shalan and Nasr 2019), electrochemical techniques (Erden et al 2016;Wei et al 2016) and chromatographic techniques such as TLC-densitometry (Patel et al 2020a, b), LC-MS/ MS (Rezk and Badr 2020) and HPLC (Alaama et al 2015;Attimarad et al 2020;Desai et al 2019;Duraisamy and Jaganathan 2017;Ebeid et al 2015;Karadurmuş et al 2018;Mahant et al 2016;Nagamani et al 2020;Naik et al 2020;Patel et al Patel et al 2020a, b;Saleh et al 2020;Saputri et al 2018). Perindopril (PER), is an antihypertensive nonsulfhydryl angiotensin converting enzyme (ACE) inhibitor used to treat hypertension and stable coronary artery disease with nomenclature of (2S,3aS,7aS)-1-[(2S)-2-[[(2S)-1-ethoxy-1-oxopentan-2-yl]amino]propanoyl]-2,3,3a,4,5,6,7,7a-octahydroindole-2-carboxylic acid (Fig.…”