Synbiotic fermented soymilk with Weissella cibaria FB069 and xylooligosaccharides prevents proliferation in human colon cancer cells

Abstract

Aim: Studies on the anticancer effects of synbiotic fermented soymilk are rare. The aim of the present study was to evaluate the effect of synbiotic fermented soymilk supplemented with xylooligosaccharides and inoculated with Weissella cibaria FB069 (FSMXW) in the proliferation of colon cancer cell and compare it with the effect of soymilk inoculated with Lactobacillus rhamnosus GG (LGG). Methods and Results: Both FB069 and LGG were able to grow in soy-based products and rapidly reduce their pH as a result of fermentation. The addition of XOS significantly enhanced the acidification rate, viscosity and total cell concentration in fermented soymilk inoculated with W. cibaria FB069. However, the same effect was not observed following inoculation with LGG. Moreover, the synbiotic FSMXW showed higher dextran, folate, GABA and aglycone content. FSMXW inhibited the proliferation of Caco-2 and HCT116 cell lines, by reducing the transcription of MD2, TLR4, MyD88, and NF-jb. Conclusions: The synbiotic soymilk containing XOS and W. cibaria FB069 increase nutrient and functional compounds through fermentation process. Significance and Impact of the Study: Our finding suggests that W. cibaria and XOS can be potentially employed in developing functional foods and health-related products.

Comparative Study of the Efficacy of Prebiotics and Probiotics as Dietary Supplements in Rats with Gastric Ulcer

“…The levels of antioxidant parameters showed no differences in healthy rats supplemented with prebiotics (G3) as compared with control healthy rats. The effect of prebiotic addition to probiotic cultures has been reported by in vitro study of Le et al, [29], who observed an inhibition in the proliferation of Caco-2 cell lines when XOS were added to a fermented soymilk by bacteria cultures of L. rhamnoses.…”

A review on the functional properties of fermented soymilk

“…The anti-proliferative property of the synbiotic soymilk was associated with TLR4/NF-κB signaling pathway. The expression of MyD88, TLR4, NF-κB, and MD2 was reduced in synbiotic soymilk treated in HCT116 cells and Caco-2 (Le et al, 2020). Similarly, Qian et al reported the anti-proliferative activity of ethanolic extract and water extract of soymilk fermented with L. casei 16 in HT-29 cells and Caco-2 cells.…”

Hemicellulose-Derived Oligosaccharides: Emerging Prebiotics in Disease Alleviation

“…Among the SCFAs, butyrate was more lethal to CRC cells and inhibited their growth by inducing histone hyperacetylation and blocking the histone deacetylase ( 79 ). The synbiotic approach was useful in this case—a XOS and Weissella cibaria combination acted differently, where XOS significantly increased the acidification rate, while W. cibaria reduced cancer cell proliferation by inhibiting the different proteins, namely, TLR4, MyD88, MD2, and NF-κb ( 80 ). It was also reported that in some instances, XOS had a more pronounced inhibitory effect on the precancerous colon lesions than conventional oligosaccharides such as FOS and decreased the amount of aberrant crypt foci in the colon ( 81 ).…”