Hemicellulose-Derived Oligosaccharides: Emerging Prebiotics in Disease Alleviation

Jana, Uttam Kumar; Kango, Naveen; Pletschke, Brett I.

doi:10.3389/fnut.2021.670817

Cited by 49 publications

(22 citation statements)

References 111 publications

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“…Among them, the most significant change occurred in AAs, particularly those in branched-chain AAs (BCAAs, valine, and isoleucine), a-ketoglutarate family (glutamate, glutamine, and arginine), and aromatic AAs (AAAs, tryptophan and tyrosine). The mutual interplay between AAs and gut bacteria has been extensively discussed (16,(23)(24)(25). For example, as the most abundant essential AAs, the levels of BCAAs are tightly correlated with certain specific bacteria such as Akkermansia (26,27), which significantly depleted in Uox-KO mice.…”

Section: Discussionmentioning

confidence: 99%

Alteration of Gut Microbiome and Correlated Amino Acid Metabolism Contribute to Hyperuricemia and Th17-Driven Inflammation in Uox-KO Mice

et al. 2022

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Although gut dysbiosis had been demonstrated to be an important factor affecting hyperuricemia (HUA) and gout, little is known for its potential mechanistic connections. In this study, Uox-KO mice model that with spontaneously developed pronounced HUA and urate nephropathy was used to explore the pathophysiologic mechanism of microbiota alterations in HUA and gout with integrated multi-omics analysis. 16S rRNA gene sequencing was performed to characterize the characteristic bacteria, and untargeted LC/MS analysis was applied to reveal the featured metabolites. Our results showed there was a significant shift in gut microbiota composition and function in Uox-KO mice compared to WT mice and apparent metabolomics differences between the two groups. Among them, amino acids metabolism appears to play a critical role. Correlation analysis further revealed that the characteristic metabolites were strongly influenced by the discrepant bacterial genera. Furthermore, impairment of intestinal integrity and profound alterations in the profile of solute carrier family resulted in dysregulation of amino acids transportation, which subsequently impacted serum uric acid level and CD4+ Th17 driven inflammation. Together, these data indicate that gut dysbiosis promotes purine metabolism disorder and inflammation in Uox-KO mice. Remodeling the gut microbiota is a promising strategy to combat HUA and gout.

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Section: Discussionmentioning

confidence: 99%

Alteration of Gut Microbiome and Correlated Amino Acid Metabolism Contribute to Hyperuricemia and Th17-Driven Inflammation in Uox-KO Mice

et al. 2022

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“…A covariance matrix was obtained from the atomic fluctuation after separating the rotational and translational movement and it was represented by the simple linear transformation in Cartesian coordinate space. Further, diagonalization of the matrix (C ij ) in PCA was obtained through an orthogonal matrix by computing on the basis of Eq (1).…”

Section: Principal Component Analysis Principal Component Analysis (Pcamentioning

confidence: 99%

“…Prebiotic oligosaccharides are non-digestible carbohydrates that confer numerous health benefits and have become a recent center of attention. These compounds are emerging as an alternative to antibiotics and may play a crucial role in mitigating the emergence of anti-microbial resistance in the host [1,2]. Different oligosaccharides available in the market are obtained either by extraction from plants or derived by enzymatic hydrolysis of various polysaccharides.…”

Section: Introductionmentioning

confidence: 99%

Molecular insight into Aspergillus oryzae β-mannanase interacting with mannotriose revealed by molecular dynamic simulation study

et al. 2022

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Fungal β-mannanases hydrolyze β-1, 4-glycosidic bonds of mannans and find application in the generation of mannose and prebiotic mannooligosaccharides (MOS). Previously, a MOS generating β-mannanase from Aspergillus oryzae MTCC 1846 (βManAo) was characterized and its structural and functional properties were unraveled through homology modeling and molecular dynamics in this study. The βManAo model was validated with 92.9% and 6.5% of the residues found to be distributed in the most favorable and allowed regions of the Ramachandran plot. Glu244 was found to play a key role in the interaction with mannotriose, indicating conserved amino acids for the catalytic reaction. A detailed metadynamic analysis of the principal components revealed the presence of an α 8 -helix in the C-terminus which was very flexible in nature and energy landscapes suggested high conformation sub-states and the complex dynamic behavior of the protein. The binding of the M3 substrate stabilized the β-mannanase and resulted in a reduction in the intermediate conformational sub-states evident from the free energy landscapes. The active site of the β-mannanase is mostly hydrophilic in nature which is accordance with our results, where the major contribution in the binding energy of the substrate with the active site is from electrostatic interactions. Define Secondary Structure of Proteins (DSSP) analysis revealed a major transition of the protein from helix to β-turn for binding with the mannotriose. The molecular dynamics of the βManAo-mannotriose model, and the role and interactions of catalytic residues with ligand were also described. The substrate binding pocket of βManAo was found to be highly dynamic and showed large, concerted movements. The outcomes of the present study can be exploited in further understanding the structural properties and functional dynamics of βManAo.

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“…Likewise, oligosaccharides derived from hemicelluloses play a role in alleviating autoimmune diseases, urinary tract infections, diabetes, Antimicrobial Resistance (AMR), and cardiovascular diseases. Thus, these DF fractions have similar potential to conventional prebiotics and can be supplemented into functional foods (Jana et al 2021). On the other hand, cellulose, as the insoluble DF fraction, has an excellent role in bodyweight management due to its waterholding capacity, where the swelled cellulose can improve satiety (Phirom-on & Apiraksakorn 2021).…”

Section: Fractionation and Physical Characteristics Of Df Fractionsmentioning

confidence: 99%

Alternative Dietary Fiber Sources from Kenaf (Hibiscus cannabinus L.) Seeds and Their by-Products

Nevara¹,

Muhammad²,

Zawawi³

et al. 2022

Jgizipangan

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This study evaluates the macronutrients and Dietary Fiber (DF) of kenaf seeds and their secondary byproducts to promote food sustainability and support the zero-waste concept. The first part concentrates on macronutrients and potential DF sources of kenaf seeds and their by-products, i.e., kenaf seed meals and dregs. Following this, the DF from the most probable source was fractionated to quantify its composition. The results showed that the macronutrients of kenaf seeds are comparable to other commercial oilseeds such as soybean, almond, and hemp seeds. Additionally, the secondary by-products could be reused as DF sources. It was found that the kenaf seed by-products had 20.63-35.08% DF contents which were comparable to soybean by-products. Moreover, the fractionation of DF from kenaf seed dregs showed that the DF comprised 1.86%, 1.01%, 6.33%, and 66.33% (dry basis) of acid-soluble pectin, calcium-bound pectin, alkali-soluble hemicellulose, and cellulose, respectively. The soluble (pectins and hemicelluloses) and insoluble (cellulose) fractions are related to the modulation of gut microbiota which have similar potential to conventional prebiotics and an excellent role in bodyweight management, respectively. These findings provide useful information for researchers and industries to venture into alternative DF sources from kenaf seeds as a value-add ingredient for functional food applications.

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Hemicellulose-Derived Oligosaccharides: Emerging Prebiotics in Disease Alleviation

Cited by 49 publications

References 111 publications

Alteration of Gut Microbiome and Correlated Amino Acid Metabolism Contribute to Hyperuricemia and Th17-Driven Inflammation in Uox-KO Mice

Alteration of Gut Microbiome and Correlated Amino Acid Metabolism Contribute to Hyperuricemia and Th17-Driven Inflammation in Uox-KO Mice

Molecular insight into Aspergillus oryzae β-mannanase interacting with mannotriose revealed by molecular dynamic simulation study

Alternative Dietary Fiber Sources from Kenaf (Hibiscus cannabinus L.) Seeds and Their by-Products

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