Symbiotic relationship between<i>Rhizobium leguminosarum</i>biovar<i>trifoliiand Trifolium nigrescens</i>

Pryor, H. N.; Lowther, W. L.

doi:10.1080/00288233.2002.9513504

Cited by 4 publications

(3 citation statements)

References 6 publications

(9 reference statements)

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“…Polyphenols were extracted following a previously published procedure [37] with some modifications. Briefly, 25 mg of pulverized samples were extracted in 1.5 mL of 75% (v/v) methanol containing 0.05% (v/v) trifluoroacetic acid (TFA), and then incubated at room temperature for one hour, in a continuous orbital shaker at a medium speed.…”

Section: Polyphenol Extractionmentioning

confidence: 99%

“…Leukemia is one of the most common tumors, with 8990 new cases and 1140 deaths from chronic myelogenous leukemia (CML) estimated to occur in the United States in 2019 (https://seer.cancer.gov/statfacts/html/cmyl.html) [2]. CML is a hematopoietic disease that mainly affects men (58% men and 42% women) between 50 and in atmospheric nitrogen fixation, as it contains nitrogen-fixing bacteria in its roots, which form nodules [37][38][39]. Nutritionally, it is a source of proteins and sugars, has a low fiber and high mineral content, and is rich in polyphenols.…”

Section: Introductionmentioning

confidence: 99%

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Trifolium Repens Blocks Proliferation in Chronic Myelogenous Leukemia via the BCR-ABL/STAT5 Pathway

Sarno

Pepe

Termolino

et al. 2020

Cells

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Some species of clover are reported to have beneficial effects in human diseases. However, little is known about the activity of the forage plant Trifolium repens, or white clover, which has been recently found to exert a hepatoprotective action. Scientific interest is increasingly focused on identifying new drugs, especially natural products and their derivatives, to treat human diseases including cancer. We analyzed the anticancer effects of T. repens in several cancer cell lines. The phytochemical components of T. repens were first extracted in a methanol solution and then separated into four fractions by ultra-high-performance liquid chromatography. The effects of the total extract and each fraction on cancer cell proliferation were analyzed by MTT assay and Western blotting. T. repens and, more robustly, its isoflavonoid-rich fraction showed high cytotoxic effects in chronic myelogenous leukemia (CML) K562 cells, with IC50 values of 1.67 and 0.092 mg/mL, respectively. The block of cell growth was associated with a total inhibition of BCR-ABL/STAT5 and activation of the p38 signaling pathways. In contrast, these strongly cytotoxic effects did not occur in normal cells. Our findings suggest that the development of novel compounds derived from phytochemical molecules contained in Trifolium might lead to the identification of new therapeutic agents active against CML.

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Section: Polyphenol Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Trifolium Repens Blocks Proliferation in Chronic Myelogenous Leukemia via the BCR-ABL/STAT5 Pathway

Sarno

Pepe

Termolino

et al. 2020

Cells

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“…White clover (Trifolium repens L.) is common fodder crop for cattle and race animals. White clover also plays a potential role in atmospheric nitrogen fixation as it contains nitrogen-fixing bacteria in its roots forming nodules (Pryor and Lowther, 2002), and it contains high levels of proteins, sugar contents, low fiber contents, and high mineral contents. However, production of white clover is affected by water shortage conditions (Li et al, 2013).…”

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confidence: 99%

Thiamin-induced variations in oxidative defense processes in white clover ( Trifolium repens L.) under water deficit stress

Ghafar¹,

Akram²,

Ashraf³

et al. 2019

Turk J Bot

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Introduction World food security is being significantly hampered due to water scarcity (Mitter et al., 2015). Water stress is different from other stresses because unlike other natural disasters its effects remain even after its period terminates (Ashraf et al., 2010; Strauss et al., 2013). Soil water deficiency affects three major mechanisms in plants: reduced canopy absorption of photosynthetically available radiation (PAR), radiation use efficiency, and harvest index, and all of these lead to reduced plant yield (Fiala et al., 2014). Seedling growth and productivity are also severely affected, mainly due to low supply of water (Atkinson and Urwin, 2012). Due to drought stress, germination potential (Wang et al., 2011), hypocotyl length, and plant biomass are commonly hampered (Wang et al., 2015). During the initial stage of growth and germination, relative water content was high in wheat, which decreased as dry matter accumulated and leaf matured (Hajheidari et al., 2007; Kaur et al., 2007). Nutrient uptake and tissue level concentrations along with water relations are perturbed under water-limited conditions in crop plants (Losak et al., 2010). Drought stress causes the production of reactive oxygen species (ROS), which affect the ultrastructure of cell membranes and DNA as well as the functioning of cellular processes (Turkan, 2011; Sun et al., 2013; Kaya et al., 2015). ROS including hydroxyl radical, anion radical, hydrogen peroxide, alkyl radicals, and singlet oxygen are injurious to plant metabolic activities. These species may react to lipids, proteins, and DNA, which causes oxidative damage and abnormal functions of cells (Sarvajeet and Tuteja, 2010; Shafiq et al., 2015). It is known that absorption of thiamin by plant root, leaves, and

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Trifolium

Williams

Nichols²

2010

Wild Crop Relatives: Genomic and Breeding Resources

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Symbiotic relationship betweenRhizobium leguminosarumbiovartrifoliiand Trifolium nigrescens

Cited by 4 publications

References 6 publications

Trifolium Repens Blocks Proliferation in Chronic Myelogenous Leukemia via the BCR-ABL/STAT5 Pathway

Trifolium Repens Blocks Proliferation in Chronic Myelogenous Leukemia via the BCR-ABL/STAT5 Pathway

Thiamin-induced variations in oxidative defense processes in white clover ( Trifolium repens L.) under water deficit stress

Trifolium

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