Symbiotic effectiveness and plant growh promoting traits in some Rhizobium strains isolated from Phaseolus vulgaris L.

Abbaszadeh‐Dahaji, Payman; Savaghebi, G.; Asadi-Rahmani, H.; Rejali, Farhad; Farahbakhsh, Mohsen; Moteshareh-zadeh, B.; Omidvari, Mahtab; Lindström, Kristina

doi:10.1007/s10725-012-9724-0

Cited by 10 publications

(5 citation statements)

References 34 publications

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“…The ability to solubilize Zn under in vitro conditions is therefore not restricted to a few bacterial genera. This is in agreement with previous studies, where isolates belonging to various bacterial genera were reported as Zn solubilizers, including Acinetobacter (47), Exiguobacterium (48), Gluconacetobacter (18), Pseudomonas (16,17), Rhizobium (33), and Serratia (49).…”

Section: Discussionsupporting

confidence: 93%

“…The formation of clearing zones around the colonies demonstrates the ability of the isolate to solubilize Zn. Microbiological media used to this end include Bunt and Rovira (29,30) and Pikovskaya (31)(32)(33)(34) media and many more (22,(35)(36)(37). Therefore, ZSB are generally not directly recovered from environmental samples but rather are screened and identified among numerous bacterial isolates (i.e., via an indirect isolation procedure).…”

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confidence: 99%

“…Therefore, ZSB are generally not directly recovered from environmental samples but rather are screened and identified among numerous bacterial isolates (i.e., via an indirect isolation procedure). This screening procedure may turn out to be costly and time consuming, as very often, ZSB represent only a small proportion of the tested bacteria (33,(38)(39)(40).…”

mentioning

confidence: 99%

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Identification of Heterotrophic Zinc Mobilization Processes among Bacterial Strains Isolated from Wheat Rhizosphere (Triticum aestivum L.)

Costerousse

Schönholzer-Mauclaire

Frossard

et al. 2018

Appl Environ Microbiol

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Soil and plant inoculation with heterotrophic zinc-solubilizing bacteria (ZSB) is considered a promising approach for increasing zinc (Zn) phytoavailability and enhancing crop growth and nutritional quality. Nevertheless, it is necessary to understand the underlying bacterial solubilization processes to predict their repeatability in inoculation strategies. Acidification via gluconic acid production remains the most reported process. In this study, wheat rhizosphere soil serial dilutions were plated on several solid microbiological media supplemented with scarcely soluble Zn oxide (ZnO), and 115 putative Zn-solubilizing isolates were directly detected based on the formation of solubilization halos around the colonies. Eight strains were selected based on their Zn solubilization efficiency and siderophore production capacity. These included one strain of , two of, three strains of , one of, and one strain of In ZnO liquid solubilization assays, the presence of glucose clearly stimulated organic acid production, leading to medium acidification and ZnO solubilization. While solubilization by and was attributed to the accumulated production of six and seven different organic acids, respectively, the other strains solubilized Zn via gluconic, malonic, and oxalic acids exclusively. In contrast, in the absence of glucose, ZnO dissolution resulted from proton extrusion (e.g., via ammonia consumption by strains) and complexation processes (i.e., complexation with glutamic acid in cultures of ). Therefore, while gluconic acid production was described as a major Zn solubilization mechanism in the literature, this study goes beyond and shows that solubilization mechanisms vary among ZSB and are strongly affected by growth conditions. Barriers toward a better understanding of the mechanisms underlying zinc (Zn) solubilization by bacteria include the lack of methodological tools for isolation, discrimination, and identification of such organisms. Our study proposes a direct bacterial isolation procedure, which prevents the need to screen numerous bacterial candidates (for which the ability to solubilize Zn is unknown) for recovering Zn-solubilizing bacteria (ZSB). Moreover, we confirm the potential of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) as a quick and accurate tool for the identification and discrimination of environmental bacterial isolates. This work also describes various Zn solubilization processes used by wheat rhizosphere bacteria, including proton extrusion and the production of different organic acids among bacterial strains. These processes were also clearly affected by growth conditions (i.e., solid versus liquid cultures and the presence and absence of glucose). Although highlighted mechanisms may have significant effects at the soil-plant interface, these should only be transposed cautiously to real ecological situations.

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Section: Discussionsupporting

confidence: 93%

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confidence: 99%

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Identification of Heterotrophic Zinc Mobilization Processes among Bacterial Strains Isolated from Wheat Rhizosphere (Triticum aestivum L.)

Costerousse

Schönholzer-Mauclaire

Frossard

et al. 2018

Appl Environ Microbiol

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“…Quantity of P solubilized by the present isolates is comparable to the one reported by Marra et al (2011) (a maximum of 95 μg/ml) for the same crop although there is difference in bacterial species. The amount of bioavailable P in liquid culture medium was inversely correlated (r=-0.731; p<0.01) to pH of culture filtrate, indicating the importance of acid production during the solubilization process that is the characteristics of fastgrowing rhizobacterial strains (Abbaszadeh et al, 2012). Marra et al (2011) also reported inorganic PS as common properties of acid producing which are fast-growing cowpea rhizobacterial strains.…”

Section: Colony Diameter (Mm)mentioning

confidence: 97%

In vitro Inorganic Phosphate Solubilization Tests of Cowpea Root Nodule Bacteria from Ethiopia

Kenasa

Nandeshwar

Assefa

2020

ASD

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Background: Phosphorus is one of the limiting plant nutrients in most soil due to its fixation with metal ions both in acidic and alkaline soil. However, some soil bacteria have potential to solubilize the cation-fixed phosphorus and make it available to plants.Methods: Sixty-six cowpea root nodule bacterial species were tested for inorganic phosphate solubilization (PS). PS potential of the isolates from Ca3 (PO4)2, AlPO4 and FePO4 were tested on four different kinds of agar media. Solublization efficiency was determined as Solubilization Index (SI) on agar media and quantity of bioavailable phosphorous on broth media. Result: About 30% of the bacterial isolates, out of which 60% endophytes were Ca3 (PO4)2 solubilizers on Pikovskaya medium but did not grow on media containing AlPO4 and FePO4 as sole P sources. Isolates showed significant variation (p less than 0.05) in Ca3 (PO4)2 solubilization efficiency on the different culture media and ECE-21 (Pseudomonas putida) was versatile in solubilizing Ca3(PO4)2 from the four test media. However, ECE-26A (Bacillus subtilis) showed the highest PS in broth media (140.8 µg ml-1) indicating the moderate correlation (r=0.5578; p less than 0.05) of PS between solid and liquid media. Amounts of P solubilized and pH change of the culture filtrate was inversely correlated (r=-0.731; p less than 0.01). In general, ECE-21 was the most efficient P solubilizer (SI=2.67±0.1; P=87.74±7.4µg ml-1) solublizers and hence can be an ideal candidate as inoculants for the crop production in Ethiopia after field evaluation.

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“…Fifty-three isolates were sampled from common bean root nodules cultivated in different locations in Iran, and half of them were placed into either R. gallicum or R. tropici type B phylogenetic groups, according to the RFLP analysis of the 16S rRNA gene [111]. Despite the fact that an accurate identification of these strains is still pending, they seem to be native of Iran, and showed a good symbiotic effectiveness as well as some plant-growth promoting traits such as P solubilization and production of auxins and siderophores when inoculated in common bean [112].…”

Section: Exploring the Natural Diversity Of Rhizobia Nodulating Commomentioning

confidence: 99%

Natural rhizobial diversity helps to reveal genes and QTLs associated with biological nitrogen fixation in common bean

2017

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Common bean is one of the most important crops for human feed, and the most important legume for direct consumption by millions of people, especially in developing countries. It is a promiscuous host legume in terms of nodulation, able to associate with a broad and diverse range of rhizobia, although the competitiveness for nodulation and the nitrogen fixation capacity of most of these strains is generally low. As a result, common bean is very inefficient for symbiotic nitrogen fixation, and nitrogen has to be supplied with chemical fertilizers. In the last years, symbiotic nitrogen fixation has received increasing attention as a sustainable alternative to nitrogen fertilizers, and also as a more economic and available one in poor countries. Therefore, optimization of nitrogen fixation of bean-rhizobia symbioses and selection of efficient rhizobial strains should be a priority, which begins with the study of the natural diversity of the symbioses and the rhizobial populations associated. Natural rhizobia biodiversity that nodulates common bean may be a source of adaptive alleles acting through phenotypic plasticity. Crosses between accessions differing for nitrogen fixation may combine alleles that never meet in nature. Another way to discover adaptive genes is to use association genetics to identify loci that common bean plants use for enhanced biological nitrogen fixation and, in consequence, for marker assisted selection for genetic improvement of symbiotic nitrogen fixation. In this review, rhizobial biodiversity resources will be discussed, together with what is known about the loci that underlie such genetic variation, and the potential candidate genes that may influence the symbiosis' fitness benefits, thus achieving an optimal nitrogen fixation capacity in order to help reduce reliance on nitrogen fertilizers in common bean.

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Symbiotic effectiveness and plant growh promoting traits in some Rhizobium strains isolated from Phaseolus vulgaris L.

Cited by 10 publications

References 34 publications

Identification of Heterotrophic Zinc Mobilization Processes among Bacterial Strains Isolated from Wheat Rhizosphere (Triticum aestivum L.)

Identification of Heterotrophic Zinc Mobilization Processes among Bacterial Strains Isolated from Wheat Rhizosphere (Triticum aestivum L.)

In vitro Inorganic Phosphate Solubilization Tests of Cowpea Root Nodule Bacteria from Ethiopia

Natural rhizobial diversity helps to reveal genes and QTLs associated with biological nitrogen fixation in common bean

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