Switchable surface coatings for control over protein adsorption

Cole, Martin A.; Jasieniak, Marek; Voelcker, Nicolas H.; Thissen, Helmut; Horn, Roger G.; Griesser, Hans J.

doi:10.1117/12.696290

Cited by 9 publications

(12 citation statements)

References 39 publications

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“…It was not among the top performers, however, and was not pursued further at the bench scale, in part due to its hydrophobic structure. Surfaces grafted with N‐ isopropylacrylamide (#51) have exhibited protein adsorption properties based on its temperature‐dependent conformation, and, therefore, could be used to control protein and cell adhesion/detachment behavior 40–47. At temperatures above its lower critical solution temperature (32°C ),43 polymer chains were collapsed and protein adsorbing whereas below this temperature they were hydrated and protein repellent 41.…”

Section: Resultsmentioning

confidence: 99%

High throughput synthesis and screening of new protein resistant surfaces for membrane filtration

et al. 2009

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A novel high throughput method for synthesis and screening of customized proteinresistant surfaces was developed. This method is an inexpensive, fast, reproducible and scalable approach to synthesize and screen protein-resistance surfaces appropriate for a specific feed. The method is illustrated here by combining a high throughput platform (HTP) approach together with our patented photo-induced graft polymerization Additional Supporting Information may be found in the online version of this article.Correspondence concerning this article should be addressed to J. E. Kilduff at kilduff@rpi.edu; or G. Belfort at belfog@rpi.edu. (PGP) method developed for facile modification of commercial poly(aryl sulfone) membranes. This new HTP-PGP method was validated by comparison with our previous published results obtained using a bench-scale filtration assay of six well-studied monomers. Optimally-performing surfaces for resisting a model protein, bovine serum albumin (BSA), were identified from a library of 66 monomers. Surfaces were prepared via graft polymerization onto poly(ether sulfone) (PES) membranes and were evaluated using a protein adsorption assay followed by pressure-driven filtration. Bench-scale verification was conducted for selected monomers using HTP-PGP method; a good correlation with HTP-PGP results was found. V

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Section: Resultsmentioning

confidence: 99%

High throughput synthesis and screening of new protein resistant surfaces for membrane filtration

et al. 2009

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“…24 XPS, however, is unsuitable for the purpose of studying protein adsorption onto pNIPAM graft coatings due to close XPS spectral similarity between pNIPAM and proteins, which prevents detection of adsorbed proteins unless there is very high coverage. This situation arises because XPS essentially performs elemental analysis, with some information also gained on next-neighbor effects; the similar elemental composition of pNIPAM and proteins, containing C, N, and O (H is not detectable by XPS) at similar proportions, causes spectral differences to be relatively small.…”

Section: Resultsmentioning

confidence: 98%

“…21,22 However, for pNIPAM, the technique of X-ray photoelectron spectroscopy (XPS) is poorly suited due to the spectral similarities with protein. 23,24 Time-of-flight-secondary ion mass spectrometry (TOF-SIMS) is an extremely sensitive analytical technique capable of identifying proteins, small molecules, and chemically modified end groups on surface tethered coatings. [25][26][27][28] TOF-SIMS has also been utilized for the detection of minute amounts of adsorbed proteins on PEG graft surfaces, undetectable by other methods.…”

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confidence: 99%

Time-of-Flight-Secondary Ion Mass Spectrometry Study of the Temperature Dependence of Protein Adsorption onto Poly(N-isopropylacrylamide) Graft Coatings

et al. 2009

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Stimuli-responsive materials show considerable promise for applications that require control over biomolecule interactions at solid material interfaces. Graft coatings of poly(N-isopropylacrylamide) (pNIPAM) are of interest for biomedical and biotechnological applications due to their temperature-dependent switching of surface properties between adhesive and nonadhesive states for cells and proteins. The characterization of protein adsorption to these switchable coatings is a formidable task since switching not only influences the affinity for proteins but at the same time induces a significant change in the coating. Here, the highly sensitive analytical technique of time-of-flight-secondary ion mass spectrometry (TOF-SIMS) combined with principal component analysis (PCA) was used for the characterization of protein adsorption onto pNIPAM coatings prepared by free radical polymerization onto surface-bound polymerizable groups. Adsorption of bovine serum albumin and lysozyme onto pNIPAM coatings from phosphate buffered solutions was investigated at temperatures above and below the polymer's lower critical solution temperature (LCST). Below the LCST, no adsorbed proteins could be detected even with this ultrasensitive method. Whereas above the LCST, adsorbed protein was detected in amounts corresponding at less than the monolayer. PCA loadings plots showed that adventitious contaminants, which might lead to confounding or misleading spectral changes upon protein exposure, were not observed.

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“…Various forms of PNIPAm appear in the literature including single chains, macroscopic gels, 21,22 microgels, 17,23À27 latex, 28 thin films, 29,30 membranes, 31 coating, 32,33 and fibers. 34,35 Early research focused on the theoretical aspects of LSCT, where PNIPAm conformation undergoes the change from a coil-toglobular structure in diluted aqueous solutions, 13À15,36 whereas more recent work has emphasized a wide range of exciting applications.…”

Section: Introductionmentioning

confidence: 99%

Multicore–Shell PNIPAm-co-PEGMa Microcapsules for Cell Encapsulation

Trongsatitkul

Budhlall

2011

Langmuir

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The overall goal of this study was to fabricate multifunctional core-shell microcapsules with biological cells encapsulated within the polymer shell. Biocompatible temperature responsive microcapsules comprised of silicone oil droplets (multicores) and yeast cells embedded in a polymer matrix (shell) were prepared using a novel microarray approach. The cross-linked polymer shell and silicone multicores were formed in situ via photopolymerization of either poly(N-isopropylacryamide)(PNIPAm) or PNIPAm, copolymerized with poly(ethylene glycol monomethyl ether monomethacrylate) (PEGMa) within the droplets of an oil-in-water-in-oil double emulsion. An optimized recipe yielded a multicore-shell morphology, which was characterized by optical and laser scanning confocal microscopy (LSCM) and theoretically confirmed by spreading coefficient calculations. Spreading coefficients were calculated from interfacial tension and contact angle measurements as well as from the determination of the Hamaker constants and the pair potential energies. The effects of the presence of PEGMa, its molecular weight (M(n) 300 and 1100 g/mol), and concentration (10, 20, and 30 wt %) were also investigated, and they were found not to significantly alter the morphology of the microcapsules. They were found, however, to significantly improve the viability of the yeast cells, which were encapsulated within PNIPAm-based microcapsules by direct incorporation into the monomer solutions, prior to polymerization. Under LSCM, the fluorescence staining for live and dead cells showed a 30% viability of yeast cells entrapped within the PNIPAm matrix after 45 min of photopolymerization, but an improvement to 60% viability in the presence of PEGMa. The thermoresponsive behavior of the microcapsules allows the silicone oil cores to be irreversibly ejected, and so the role of the silicone oil is 2-fold. It facilitates multifunctionality in the microcapsule by first being used as a template to obtain the desired core-shell morphology, and second it can act as an encapsulant for oil-soluble drugs. It was shown that the encapsulated oil droplets were expelled above the volume phase transition temperature of the polymer, while the collapsed microcapsule remained intact. When these microcapsules were reswollen with an aqueous solution, it was observed that the hollow compartments refilled. In principle, these hollow-core microcapsules could then be filled with water-soluble drugs that could be delivered in vivo in response to temperature.

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Switchable surface coatings for control over protein adsorption

Cited by 9 publications

References 39 publications

High throughput synthesis and screening of new protein resistant surfaces for membrane filtration

High throughput synthesis and screening of new protein resistant surfaces for membrane filtration

Time-of-Flight-Secondary Ion Mass Spectrometry Study of the Temperature Dependence of Protein Adsorption onto Poly(N-isopropylacrylamide) Graft Coatings

Multicore–Shell PNIPAm-co-PEGMa Microcapsules for Cell Encapsulation

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