Swinepox Virus as a Vector for the Delivery of Immunogens

Foley, Patricia L.; Paul, P. S.; Levings, Randall L.; Hanson, S.K.; Middle, L.A.

doi:10.1111/j.1749-6632.1991.tb18581.x

Cited by 10 publications

(2 citation statements)

References 2 publications

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“…Recombinant poxviruses, including swinepox virus, are strong candidates as live viral vectors for use in humans and animals [34][35][36][37]. SWPV has several competitive advantages as a viral vector for pig immunization.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Swinepox Virus from Outbreak in Russia

Koltsov

Сухер

Kholod

et al. 2023

Animals

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Swinepox virus (SWPV) is the only member of the Suipoxvirus genus of the Poxviridae family and is an etiologic agent of a worldwide disease specific for domestic and wild pigs. SWPV outbreaks are sporadically recorded in different regions of Russia. In 2013, an outbreak of the disease causing skin lesions was registered on a pig farm in Russia. The presence of SWPV in the scab samples was assessed by in-house real-time PCR, reference PCR amplification, and nucleotide sequencing of the viral late transcription factor-3 (VLTF-3) gene and was then confirmed by virus isolation. Thus, the in-house real-time PCR proposed in this study could serve as a useful tool for the rapid specific detection of the swinepox virus. In the study, it has been demonstrated for the first time that nasal and oral swabs can be used for PCR diagnosis of the disease and for swinepox virus isolation. Phylogenetic analysis revealed that the isolated virus was closely related to SWPV isolates registered in Germany, USA, and Brazil, and slightly differed from the Indian isolates. During experimental infection of pigs, a low pathogenicity of the Russian isolate was observed. Our data provides the first report on the isolation and characterization of swinepox virus in Russia.

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Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Swinepox Virus from Outbreak in Russia

Koltsov

Сухер

Kholod

et al. 2023

Animals

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“…Swinepox virus (SPV) is the sole member of the genus Suipoxvirus , belonging to the family Poxviridae . Its biological characteristics, and particularly its narrow host range, make it a promising vector for recombinant vaccines for swine (Foley et al ., 1991 ; Tuboly et al ., 1993 ; van der Leek et al ., 1994 ).…”

Section: Introductionmentioning

confidence: 99%

Sequence and analysis of a swinepox virus homologue of the vaccinia virus major envelope protein P37 (F13L)

Bárcena

Lorenzo

Sánchez-Puig

et al. 2000

Journal of General Virology

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P37 (F13L gene product), the most abundant protein in the envelope of the extracellular virus form of the prototype poxvirus, vaccinia virus (VV), is a crucial player in the process leading to acquisition of the envelope, virus egress and transmission. We have cloned and sequenced a swinepox virus (SPV) gene homologous to VV F13L. The SPV gene product, termed P42, was 54 % identical to P37, the VV F13L gene product, and, among the poxviruses, was most similar (73 % identity) to the myxoma virus homologue. The SPV P42 gene contained late transcription signals and was expressed only at late times during infection. The protein was palmitylated, and showed an intracellular distribution similar to that of VV P37, both by immunofluorescence and by subcellular fractionation. As with VV P37, SPV P42 was incorporated in extracellular enveloped SPV particles, but was absent from the intracellular mature virus form. To check the ability of SPV P42 to function in the context of VV infection, we inserted the SPV gene into a VV deficient in P37, which is severely blocked in virus envelopment and cell-to-cell transmission. Despite correct expression of SPV P42, the resulting recombinant VV showed no rescue of extracellular virus formation or cell-to-cell virus spread. The lack of function of SPV P42 in the VV genetic background suggests that specific interactions between SPV P42 or VV P37 and other viral proteins is required to drive the envelopment process.

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Swinepox Virus

Freitas¹

2019

Diseases of Swine

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Swinepox Virus as a Vector for the Delivery of Immunogens

Cited by 10 publications

References 2 publications

Isolation and Characterization of Swinepox Virus from Outbreak in Russia

Isolation and Characterization of Swinepox Virus from Outbreak in Russia

Sequence and analysis of a swinepox virus homologue of the vaccinia virus major envelope protein P37 (F13L)

Swinepox Virus

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