Sequence and analysis of a swinepox virus homologue of the vaccinia virus major envelope protein P37 (F13L)

Bárcena, Juan; Lorenzo, Marı́a M.; Sánchez-Puig, Juana M.; Blasco, Rafael

doi:10.1099/0022-1317-81-4-1073

Cited by 15 publications

(9 citation statements)

References 44 publications

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“…pMVA-Gus was constructed by replacing the rsGFP cassette with a fragment containing a polylinker, the sequence of the -glucuronidase gene, and a synthetic vaccinia virus early/late promoter from plasmid pRB-Gus (20). This was achieved by cloning an Xho I/ Bam HI fragment from plasmid pRB-Gus into the Xho I/ Bam HI sites of plasmid pMVA-rsGFP.…”

Section: Methodsmentioning

confidence: 99%

Isolation of Vaccinia MVA Recombinants Using the Viral F13L Gene as the Selective Marker

Sánchez-Puig¹,

Blasco²

2005

BioTechniques

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Modified vaccinia Ankara (MVA) is a highly attenuated vaccine vector that has an excellent vaccine safety record. Also, as a eukaryotic gene expression vector, MVA can be used in a biosafety level 1 setup, in contrast to more virulent vaccinia virus strains. Isolation of recombinant MVA involves repeated plaquing of the virus and is burdensome because virus plaques are slow to develop and difficult to recognize. To facilitate the generation of MVA recombinants, we have developed a cloning system for MVA based on the selection of the viral F13L gene. Deletion of F13L in MVA produced a small plaque phenotype and a reduction in extracellular virus formation, indicating a severe block in cell-to-cell spread. When using the F13L knockout virus as the parental virus, reintroduction of the F13L gene in the original locus was used as an efficient selection for the isolation of virus recombinants. The selection procedure can be done entirely in the permissive baby hamster kidney (BHK)-21 cell line, does not require plaque isolation, and rendered close to 100% recombinant virus.

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Section: Methodsmentioning

confidence: 99%

Isolation of Vaccinia MVA Recombinants Using the Viral F13L Gene as the Selective Marker

Sánchez-Puig¹,

Blasco²

2005

BioTechniques

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“…This region is a homologue of the major envelope antigen of vaccinia virus and one of a limited number of ORFV proteins to which sheep mount a strong antibody response and which stimulate lymphocytes (Sullivan et al , 1994 ). The region also encodes an envelope protein in other viruses belonging to the family Poxviridae , such as molluscum contagiosum virus (Blake et al , 1991 ), fowlpox virus (Calvert et al , 1992 ), swinepox virus (Bárcena et al , 2000 ) and sheeppox virus (H. G. Heine, M. F. Rudd, A. J. Foord and D. B. Boyle, unpublished data). Generally, the virus envelope antigen is exposed to immunological pressure from the host and tends to be variable.…”

Section: Full Textmentioning

confidence: 99%

Genetic heterogeneity among parapoxviruses isolated from sheep, cattle and Japanese serows (Capricornis crispus)

Inoshima

Murakami

Yokoyama

et al. 2001

Journal of General Virology

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Standard strains of four parapoxviruses and seven unclassified Japanese strains isolated from sheep, cattle and wild Japanese serows (Capricornis crispus) were compared molecularly. Restriction fragment length polymorphism (RFLP) analysis of viral DNA, indirect immunofluorescence assays using monoclonal antibodies, partial nucleotide sequencing of the envelope gene, phylogenetic analysis and PCR-RFLP were carried out. These analyses revealed that the parapoxviruses were divided into four groups and the region sequenced in this study was highly conserved within each group. Each of the Japanese isolates was classified into one of these groups. These findings also indicated that parapoxvirus infections among wild Japanese serows seem to be caused by at least two different parapoxviruses, bovine papular stomatitis virus and orf virus. The methods presented here are useful for genetic characterization and classification of parapoxviruses.The genus Parapoxvirus in the family Poxviridae has four members, orf virus (ORFV), bovine papular stomatitis virus (BPSV), pseudocowpox virus (PCPV) and parapoxvirus of red deer in New Zealand (PVNZ). Parapoxvirus infections are widespread in ruminants worldwide. Parapoxviruses generally induce mild papular dermatitis around the mouth, teats and skin of affected animals such as sheep, goats, cattle and some wild species (Esposito et al

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“…For this, plasmid pRB21-βGus [32] was transfected into cells infected with virus vRB12 [33], and recombinant viruses were subsequently isolated by plaque selection [34]. Vaccinia virus expressing Sp6 DNA polymerase vSIMBE/L [35] was kindly made available by B. Moss.…”

Section: Methodsmentioning

confidence: 99%

A Vaccinia Virus Recombinant Transcribing an Alphavirus Replicon and Expressing Alphavirus Structural Proteins Leads to Packaging of Alphavirus Infectious Single Cycle Particles

2013

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Poxviruses and Alphaviruses constitute two promising viral vectors that have been used extensively as expression systems, or as vehicles for vaccine purposes. Poxviruses, like vaccinia virus (VV) are well-established vaccine vectors having large insertion capacity, excellent stability, and ease of administration. In turn, replicons derived from Alphaviruses like Semliki Forest virus (SFV) are potent protein expression and immunization vectors but stocks are difficult to produce and maintain. In an attempt to demonstrate the use of a Poxvirus as a means for the delivery of small vaccine vectors, we have constructed and characterized VV/SFV hybrid vectors. A SFV replicon cDNA was inserted in the VV genome and placed under the control of a VV early promoter. The replicon, transcribed from the VV genome as an early transcript, was functional, and thus capable of initiating its own replication and transcription. Further, we constructed a VV recombinant additionally expressing the SFV structural proteins under the control of a vaccinia synthetic early/late promoter. Infection with this recombinant produced concurrent transcription of the replicon and expression of SFV structural proteins, and led to the generation of replicon-containing SFV particles that were released to the medium and were able to infect additional cells. This combined VV/SFV system in a single virus allows the use of VV as a SFV delivery vehicle in vivo. The combination of two vectors, and the possibility of generating in vivo single-cycle, replicon containing alphavirus particles, may open new strategies in vaccine development or in the design of oncolytic viruses.

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Sequence and analysis of a swinepox virus homologue of the vaccinia virus major envelope protein P37 (F13L)

Cited by 15 publications

References 44 publications

Isolation of Vaccinia MVA Recombinants Using the Viral F13L Gene as the Selective Marker

Isolation of Vaccinia MVA Recombinants Using the Viral F13L Gene as the Selective Marker

Genetic heterogeneity among parapoxviruses isolated from sheep, cattle and Japanese serows (Capricornis crispus)

A Vaccinia Virus Recombinant Transcribing an Alphavirus Replicon and Expressing Alphavirus Structural Proteins Leads to Packaging of Alphavirus Infectious Single Cycle Particles

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