Swainsonine production in fed-batch fermentations of Metarhizium anisopliae

Patrick, Manjula S.; Adlard, Maxwell W.; Keshavarz, Tajalli

doi:10.1007/bf00130803

Cited by 14 publications

(2 citation statements)

References 11 publications

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“…A regulatory influence of pH shift on the production of both primary [13] and secondary metabolites [14] has been demonstrated. A pH range of 3 -7 was maintained in the optimized media obtained up to this step.…”

Section: Effect Of Phmentioning

confidence: 99%

Optimization of different process variables for the production of an indolizidine alkaloid, swainsonine from Metarhizium anisopliae

Singh

Kaur

2011

J. Basic Microbiol.

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Swainsonine is a polyhydroxylated indolizidine alkaloid having anticancer, antimetastatic, antiproliferative and immunomodulatory activities and also potential therapeutic applications against AIDS. In the present study, ten isolates of M. anisopliae were screened and enzyme assayed for the production of swainsonine in different media (Complex oatmeal, Czapekdox media with and without lysine (8% w/v) and Sabouraud dextrose broth (SDB)). Among these strains, ARSEF 1724 (UM8) was found to produce highest amount of swainsonine (1.34 μg/l) after 72 h of incubation under shake flask conditions at 180 rpm and 28 °C in complex oatmeal media. In order to maximize the yield of swainsonine the media composition including macro and micronutrients were optimized. The process variables including the chemical factors like carbon sources, nitrogen sources of both organic and inorganic nature and pH with constant inoculum size (1 × 10(8) spores/ml) were screened using classical one-factor-at-a-time (OFAT) approach to find their optimum levels. The present study shows that the nutrient requirement is specific for each strain of Metarhizium. Oatmeal extract (6%) was found to be the best supporting media along with nitrogen source, glucose (2%) as best carbon source and pH (~5) as the best for swainsonine production.

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Section: Effect Of Phmentioning

confidence: 99%

Optimization of different process variables for the production of an indolizidine alkaloid, swainsonine from Metarhizium anisopliae

Singh

Kaur

2011

J. Basic Microbiol.

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“…Lipase production by microorganisms is mostly performed by batch cultivation processes with careful media formulation 9. 10 Fed‐batch fermentation has proved successful especially for over‐production of secondary metabolites in submerged cultures 11–13. Feeding strategies have been used not only to enhance titres and extend the duration of production directly, but also to improve culture homogeneity through reduction of viscosity and improved oxygen transfer.…”

Section: Introductionmentioning

confidence: 99%

Lipolytic enzyme production in batch and fed-batch cultures ofOphiostoma piceae andFusarium oxysporum

Tamerler

Keshavarz

2000

J. Chem. Technol. Biotechnol.

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Lipase and esterase production by Ophiostoma piceae and Fusarium oxysporum were enhanced and extended by developing a fed-batch process in stirred tank reactors. Fed-batch strategy improved lipolytic enzyme production from Ophiostoma piceae in both 2 and 20 dm 3 stirred tank reactors. However, fed-batch fermentation of Fusarium oxysporum in the 2 dm 3 reactor was more effective than both batch and fed-batch fermentations in the 20 dm 3 reactor. When a medium composed of only carbon and nitrogen source was intermittently fed to the cultures, the maximum speci®c lipase activity was improved by more than 80% and 35% in Ophiostoma piceae and Fusarium oxysporum cultures respectively. The maximum speci®c esterase activity was improved by 20% and 15% in Ophiostoma piceae and Fusarium oxysporum cultures respectively. The duration of production for both fungi extended from 144 to 216 h compared with a batch culture under the same condition.

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(‐)‐Swainsonine

2005

Synthesis of Naturally Occurring Nitrogen Heterocycles From Carbohydrates

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Swainsonine production in fed-batch fermentations of Metarhizium anisopliae

Cited by 14 publications

References 11 publications

Optimization of different process variables for the production of an indolizidine alkaloid, swainsonine from Metarhizium anisopliae

Optimization of different process variables for the production of an indolizidine alkaloid, swainsonine from Metarhizium anisopliae

Lipolytic enzyme production in batch and fed-batch cultures ofOphiostoma piceae andFusarium oxysporum

(‐)‐Swainsonine

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